| Budget Amount *help |
¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Outline of Final Research Achievements |
In porcine non-surgical embryo transfer (ET), the frequency of embryonic mortality increased significantly at 2 or 3 day after ET, which is from 6 days after ovulation. However, since there are no significant differences in mRNA levels of EGF like growth factor and ErbB receptor families between ET embryos and artificially inseminated (AI) embryos, it was suggested that early pregnancy loss was not associated with difference in their gene expression levels. Proportion of apoptotic cells in ET embryos was significantly higher than that in AI embryos. Thus, high incidence of apoptosis may cause embryonic mortality after ET. When 40 blastocysts were transferred non-surgically into recipients, their pregnancy rate was significantly higher compared to when 25 blastocysts were transferred. It may suggest that increased numbers of ET embryos maintain the number of alive embryos required for pregnancy establishment in recipients.
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