| Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2013: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Outline of Final Research Achievements |
Animal induced pluripotent stem cells (iPSCs) are required for veterinary regenerative medicine. Transformed cells appeared after introducing reprogramming factors into a dog or cat somatic cells, but they did not maintain pluripotency. Comparative metabolome analysis revealed that dog and cat cells were suffered extreme oxidative stress at the initiation of reprogramming. On the other hand, it is successful to make feeder cells from dog and cat somatic cells that can maintain pluripotency of human iPSCs. In addition, the genetic diagnosis of glutaric aciduria type II in cat was established.
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