| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Outline of Final Research Achievements |
Bovine and porcine oocyte-cumulus-granulosa cell complexes collected from early antral follicles were cultured for 14 days in a medium with appropriate concentrations of polyvinylpyrrolidone (PVP) and steroidal hormones. Complexes from both species formed dome-like (antral follicle-like) structures, in which oocytes grew to the final sizes and acquired the maturational competence. FSH and PVP promoted proliferation of porcine cumulus cells to make dome-like structures. In bovine oocyte-cumulus-granulosa cell complexes, steroidal hormones maintained connections between oocytes and cumulus cells via transzonal projections, which extended from cumulus cells through the zona pellucida to the oolemma. High concentrations of PVP down-regulated gene expression of extracellular matrices in both bovine and porcine granulosa cells surrounding oocytes. In vitro-grown bovine oocytes were similar to in vivo grown oocytes in fertilization ability and developed to blastocysts.
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