| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Outline of Final Research Achievements |
Genome editing technology is important for research of gene function. TALEN and CRISPR/ Cas9 system have been developed rapidly, and genome-modified animals have been reported in many species. Although genome-editing tool microinjections are mainly performed at one-cell stage using fertilized eggs, these procedures and in vitro fertilization are extremely difficult to perform in chickens. Therefore, the investigations of genetically modified chickens have been focused on the genetic modifications of germ cells, and the methods of gene transfer using ESCs and PGCs have been especially studied. Then, we try to create cHEMGN-knock out chickens that can keep germ line competency and genome editing tools for example, TALEN or CRISPR/Cas9. As a result, some cHEMGN knock-out ESC and PGC clones were obtained by using both TALEN- and CRISPR/Cas9-mediated methods, and fifteen germline chimeric chickens were created from transplantation experiments of cHEMGN-knock out PGC clones.
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