| Budget Amount *help |
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2013: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
|
|---|
| Outline of Final Research Achievements |
Although field-emission scanning electron microscopy (FE-SEMs) has proven very useful in biomedical research, the high vacuum required precludes direct observations of living organisms at high resolution and often produces unwanted structural changes. In this application, we improved a method that allows the investigator to keep a variety of specimens alive in the high vacuum environment of the electron microscope by encasing the organisms in a thin, vacuum-proof suit, the “NanoSuit". However, it was impossible to protect wet tissues freshly excised from intact organisms or cultured cells. We further improved the technique to overcome this limitation. We protect the specimens with a Surface Shield Enhancer (SSE) solution and find that the fine structure of the SSE treated specimens is superior to that of conventionally prepared specimens. The SSE based NanoSuit affords a much stronger barrier to gas and/or liquid loss than the previous NanoSuit did.
|
