A thin polymer membrane, NanoSuit, enhancing survival across the continuum between air and high vacuum
Project/Area Number |
25292198
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Insect science
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Research Institution | Hamamatsu University School of Medicine |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
HARIYAMA Takahiko 浜松医科大学, 医学部, 教授 (30165039)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2013: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
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Keywords | 電子顕微鏡 / 高真空 / 生きたまま / NanoSuit / FE-SEM / 形態学 |
Outline of Final Research Achievements |
Although field-emission scanning electron microscopy (FE-SEMs) has proven very useful in biomedical research, the high vacuum required precludes direct observations of living organisms at high resolution and often produces unwanted structural changes. In this application, we improved a method that allows the investigator to keep a variety of specimens alive in the high vacuum environment of the electron microscope by encasing the organisms in a thin, vacuum-proof suit, the “NanoSuit". However, it was impossible to protect wet tissues freshly excised from intact organisms or cultured cells. We further improved the technique to overcome this limitation. We protect the specimens with a Surface Shield Enhancer (SSE) solution and find that the fine structure of the SSE treated specimens is superior to that of conventionally prepared specimens. The SSE based NanoSuit affords a much stronger barrier to gas and/or liquid loss than the previous NanoSuit did.
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Report
(4 results)
Research Products
(25 results)
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[Presentation] Dressing living organisms by a thin polymer membrane, NanoSuit, for the FE-SEM observation2014
Author(s)
Hariyama T, Ohta I, Hirakawa S, Kawasaki H, Suzuki A, Ishii D, Muranaka Y, Shimomura M, Takaku Y
Organizer
The 70th Annual Meeting of the Japanese Society of Microscopy
Place of Presentation
千葉 幕張メッセ
Year and Date
2014-05-11 – 2014-05-13
Related Report
Invited
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