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Development of a gene expression control system using genome editing and its application to insect control

Research Project

Project/Area Number 25292202
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Insect science
Research InstitutionNational Institute of Agrobiological Sciences

Principal Investigator

SEZUTSU HIDEKI  国立研究開発法人農業生物資源研究所, 遺伝子組換えカイコ研究開発ユニット, ユニット長 (70342805)

Co-Investigator(Kenkyū-buntansha) KOMOTO NATUO  国立研究開発法人農業生物資源研究所, 遺伝子組換えカイコ研究開発ユニット, 主任研究員 (30355747)
TOMITA SYUICHIRO  国立研究開発法人農業生物資源研究所, 遺伝子組換えカイコ研究開発ユニット, 主任研究員 (30360457)
HATAKEYAMA MASATSUGU  国立研究開発法人農業生物資源研究所, 昆虫成長制御研究ユニット, 主任研究員 (50281142)
NIIMI TERUYUKI  名古屋大学, 大学院生命農学研究科, 助教 (00293712)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywordsトランスジェニック昆虫 / ゲノム編集昆虫 / 遺伝子組換え昆虫 / ゲノムエディティング / 昆虫ゲノム編集 / 昆虫制御 / 昆虫遺伝子機能解析 / ゲノム編集
Outline of Final Research Achievements

To develop a gene expression control system using genome editing for the insect control, we had tried to establish the genome editing system in the various species of insect order Lepidoptera, Diptera, Hymenoptera, and Coleoptera. Using silkworm, Bombyx mori, we have successfully established many gene-knockout strains and studied the gene functions. In addition, we tried to develop a conditional genome editing methods using GAL4/UAS binary system or Tet-On/Off system with a specific promoter. We have also succeeded in gene knockout of a transgene by transcription activator-like effector nucleases (TALENs) in the sawfly, Athalia rosae (Hymenoptera) and the ladybird beetle, Harmonia axyridis (Coleoptera). The results suggested that the genome editing method is available among the various non-model insects.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (38 results)

All 2016 2015 2014 2013

All Journal Article (14 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 12 results,  Acknowledgement Compliant: 4 results,  Open Access: 6 results) Presentation (22 results) (of which Invited: 5 results) Book (2 results)

  • [Journal Article] Knockout of a transgene by transcription activator-like effector nucleases (TALENs) in the sawfly, Athalia rosae (Hymenoptera) and the ladybird beetle, Harmonia axyridis (Coleoptera)2016

    • Author(s)
      Hatakeyama M, Yatomi J, Sumitani M, Takasu Y, Sekiné K, Niimi T, Sezutsu H
    • Journal Title

      Insect Molecular Biology

      Volume: 25(1) Issue: 1 Pages: 24-31

    • DOI

      10.1111/imb.12195

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Positional cloning of a Bombyx pink eyed white egg locus reveals the major role of cardinal in ommochrome synthesis2016

    • Author(s)
      Osanai-Futahashi M, Tatematsu KI, Futahashi R, Narukawa J, Takasu Y, Kayukawa T, Shinoda T, Ishige T, Yajima S, Tamura T, Yamamoto K, Sezutsu H
    • Journal Title

      Heredity

      Volume: 116(2) Issue: 2 Pages: 135-145

    • DOI

      10.1038/hdy.2015.74

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Disruption of diapause induction by TALEN-based gene mutagenesis in relation to a unique neuropeptide signaling pathway in Bombyx.2015

    • Author(s)
      Shiomi, K., Takasu, Y., Kunii, M., Tsuchiya, R., Mukaida, M., Kobayashi, M., Sezutsu, H., Ichida, M., Mizoguchi, A.
    • Journal Title

      Scientific Reports

      Volume: 5 Issue: 1 Pages: 15566-15566

    • DOI

      10.1038/srep15566

    • NAID

      120007101413

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Knockout silkworms reveal a dispensable role for juvenile hormones in holometabolous life cycle2015

    • Author(s)
      Daimon T, Uchibori M, Nakao H, Sezutsu H, Shinoda T
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 112(31) Issue: 31

    • DOI

      10.1073/pnas.1506645112

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Targeted disruption of a single sex pheromone receptor gene completely abolishes in vivo pheromone response in the silkmoth2015

    • Author(s)
      Sakurai T, Mitsuno H, Mikami A, Uchino K, Tabuchi M, Zhang F, Sezutsu H, Kanzaki R
    • Journal Title

      Sci Rep

      Volume: 5 Issue: 1 Pages: 11001-11001

    • DOI

      10.1038/srep11001

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 新しい昆虫産業を創る!- カイコにおける次世代ゲノム改変技術の開発と異分野融合-2015

    • Author(s)
      瀬筒秀樹
    • Journal Title

      日本農学会編 シリーズ21世紀の農学「ここまで進んだ!飛躍する農学」

      Volume: 2015 Pages: 19-38

    • Related Report
      2015 Annual Research Report
  • [Journal Article] The boule gene is essential for spermatogenesis of haploid insect male.2015

    • Author(s)
      Sekine K, Furusawa T, Hatakeyama M
    • Journal Title

      Dev. Biol.

      Volume: 339(1) Issue: 1 Pages: 154-163

    • DOI

      10.1016/j.ydbio.2014.12.027

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Establishment of tools for neurogenetic analysis of sexual behavior in the silkmoth, Bombyx mori.2014

    • Author(s)
      Taketoshi Kiya, Koudai Morishita, Keiro Uchino, Masafumi Iwami, and Hideki Sezutsu
    • Journal Title

      PLoS One

      Volume: 9 (11) Issue: 11 Pages: e113156-e113156

    • DOI

      10.1371/journal.pone.0113156

    • NAID

      120005537889

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas92014

    • Author(s)
      S. Nakade, T. Tsubota, Y. Sakane, S. Kume, N. Sakamoto, M. Obara, T. Daimon, H. Sezutsu, T. Yamamoto, T. Sakuma and K. T. Suzuki
    • Journal Title

      Nat Commun

      Volume: 5 Issue: 1 Pages: 5560-5560

    • DOI

      10.1038/ncomms6560

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification of a novel strong and ubiquitous promoter/enhancer in the silkworm Bombyx mori2014

    • Author(s)
      Tsubota T, Uchino K, Suzuki T.K, Tanaka H, Kayukawa T, Shinoda T, Sezutsu H
    • Journal Title

      G3

      Volume: 4 Issue: 7 Pages: 1347-1357

    • DOI

      10.1534/g3.114.011643

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Establishment of transgenic silkworms expressing GAL4 specifically in the haemocyte oenocytoid cells2014

    • Author(s)
      Tsubota T, Uchino K, Kamimura M, Ishikawa M, Hamamoto H, Sekimizu K, Sezutsu H.
    • Journal Title

      Insect Mol Biol.

      Volume: 23 Issue: 2 Pages: 165

    • DOI

      10.1111/imb.12071

    • URL

      https://pure.teikyo.jp/en/publications/a5381ee8-4f74-4b6a-9219-ddd4d727c7b6

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient TALEN construction for Bombyx gene targeting.2013

    • Author(s)
      Takasu Y, Sajwan S, Daimon T, Osanai-Futahashi M, et al(他4名)
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 9 Pages: e73458-e73458

    • DOI

      10.1371/journal.pone.0073458

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PhiC31 integrase-mediated, site-specific integration of transgenes in the silkworm, Bombyx mori (Lepidoptera: Bombycidae)2013

    • Author(s)
      Yonemura N, Tamura T, Uchino K, Kobayashi I, Tatematsu KI, Iizuka T, Tsubota T, Sezutsu H
    • Journal Title

      Applied Entomology and Zoology

      Volume: 48(3) Issue: 3 Pages: 265-273

    • DOI

      10.1007/s13355-013-0182-6

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 遺伝子組換えカイコについて2013

    • Author(s)
      瀬筒秀樹
    • Journal Title

      日本絹の里紀要

      Volume: 15 Pages: 56-63

    • NAID

      40019744566

    • Related Report
      2013 Annual Research Report
  • [Presentation] 昆虫病原糸状菌Beauveria brongniartiiに対するカイコの抵抗性遺伝子の同定2016

    • Author(s)
      和田早苗、宮本和久、山本公子、高須陽子、飯塚哲也、内野恵郎、瀬筒秀樹、田中博光、国見裕久
    • Organizer
      日本昆虫学会第76回大会・第60回日本応用動物昆虫学会大会合同大会
    • Place of Presentation
      大阪府立大学
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 最新の分子遺伝学的手法を使った殺虫剤抵抗性機構解明の試み2016

    • Author(s)
      神村学、高須陽子、和田早苗、瀬筒秀樹、黒川竜紀、森泰生
    • Organizer
      日本昆虫学会第76回大会・第60回日本応用動物昆虫学会大会合同大会
    • Place of Presentation
      大阪府立大学
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] カイコの幼虫・蛹・成虫形質発現を支配する鍵遺伝子のモザイク解析2016

    • Author(s)
      大門高明、浅野(内堀)美和、瀬筒秀樹、篠田徹郎
    • Organizer
      平成28年度蚕糸・昆虫機能利用学術講演会
    • Place of Presentation
      京都工芸繊維大学
    • Year and Date
      2016-03-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] カイコにおけるコラゾニンおよびその受容体遺伝子のノックアウトが休眠性に及ぼす影響の調査2015

    • Author(s)
      渡井拓人、田中まみ、田中佑磨、土屋良磨、高須陽子、瀬筒秀樹、塩見邦博
    • Organizer
      日本蚕糸学会中部支部第71回・東海支部第67回研究発表会
    • Place of Presentation
      信州大学
    • Year and Date
      2015-12-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] 新たなカイコ産業の創出に向けて2015

    • Author(s)
      瀬筒秀樹
    • Organizer
      アグリビジネス創出フェア
    • Place of Presentation
      東京ビッグサイト
    • Year and Date
      2015-11-20
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 遺伝子組換えカイコについて - 昆虫利用の新展開 -2015

    • Author(s)
      瀬筒秀樹
    • Organizer
      NIASオープンカレッジ
    • Place of Presentation
      つくばサイエンス・インフォメーションセンター
    • Year and Date
      2015-10-28
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 一本鎖DNAドナーを用いたカイコ遺伝子の改変2015

    • Author(s)
      高須陽子、田村俊樹、小林功、張薔、内野恵郎、瀬筒秀樹
    • Organizer
      日本蚕糸学会第85回大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] PITCh(ピッチ)法を利用したカイコにおける簡便かつ効率的な遺伝子ノックイン2015

    • Author(s)
      坪田拓也、内野恵郎、高須陽子、中出翔太、坂根祐人、久米悟士、坂本尚昭、小原政信、大門高明、山本卓、佐久間哲史、鈴木賢一、瀬筒秀樹
    • Organizer
      日本蚕糸学会第85回大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] ノックアウトカイコを用いた幼若ホルモンの機能解析2015

    • Author(s)
      篠田徹郎、内堀美和、瀬筒秀樹、大門高明
    • Organizer
      昆虫ポストゲノム研究会
    • Place of Presentation
      昆虫ポストゲノム研究会
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] “新蚕業”創出に向けた基盤技術の開発と今後の展開方向2015

    • Author(s)
      瀬筒秀樹
    • Organizer
      第7回公開シンポジウム「カイコ産業の未来」, 東京
    • Place of Presentation
      国立科学博物館、東京
    • Year and Date
      2015-01-16
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] MMEJ-mediated integration of donor DNA in cells and animals using TALENs and CRISPR/Cas92015

    • Author(s)
      Nakade S, Tsubota T, Sakane Y, Kume S, Sakamoto N, Obara M, Daimon T, Sezutsu H, Yamamoto T, Sakuma T, Suzuki KT
    • Organizer
      Keystone symposia
    • Place of Presentation
      Montana, USA
    • Year and Date
      2015-01-11 – 2015-01-16
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] TALENを用いたゲノム編集によるオモクローム系色素合成経路の解明2014

    • Author(s)
      二橋美瑞子、立松謙一郎、二橋亮、高須陽子、粥川琢巳、石毛太一郎、矢嶋俊介、田村俊樹、山本公子、瀬筒秀樹
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜、横浜市
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] イコの強力なプロモーターの同定2014

    • Author(s)
      坪田拓也、内野恵郎、鈴木誉保、田中博光、粥川琢巳、篠田徹郎、瀬筒秀樹
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜、横浜市
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 新しい昆虫産業を創る!―カイコにおける次世代ゲノム改変技術の開発と異分野融合 ―2014

    • Author(s)
      瀬筒秀樹
    • Organizer
      H26年度日本農学会シンポジウム「ここまで進んだ!飛躍する農学」
    • Place of Presentation
      東京大学弥生講堂、東京
    • Year and Date
      2014-10-04
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 遺伝子組換えカイコガを用いた性フェロモン受容体遺伝子のin vivoプロモーター解析2014

    • Author(s)
      櫻井健志、田渕理史、水野依利子、瀬筒秀樹、神崎亮平
    • Organizer
      日本味と匂い学会第48回大会
    • Place of Presentation
      静岡市清水文化会館、静岡市
    • Year and Date
      2014-10-02 – 2014-10-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] Knockout studies on JH receptors and JH biosynthetic genes in the silkworm, Bombyx mori2014

    • Author(s)
      Daimon T, Uchibori M,Nakao H,Sezutsu H, Shinoda T
    • Organizer
      10th International Conference on Juvenile Hormones
    • Place of Presentation
      Tsukuba, Japan
    • Year and Date
      2014-06-09 – 2014-06-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 性フェロモン受容体遺伝子ノックアウトカイコガの生理・行動学的解析2014

    • Author(s)
      櫻井健志, 光野秀文, 内野恵郎, 三上晃久, 田渕理史, Zhang Feng, 瀬筒秀樹, 神崎亮平
    • Organizer
      第58回日本応用動物昆虫学会大会
    • Place of Presentation
      高知市文化プラザ
    • Related Report
      2013 Annual Research Report
  • [Presentation] PTTHノックアウトカイコにおける発育異常2014

    • Author(s)
      内堀美和, 大門高明, 瀬筒秀樹, 篠田徹郎
    • Organizer
      第58回日本応用動物昆虫学会大会
    • Place of Presentation
      高知市文化プラザ
    • Related Report
      2013 Annual Research Report
  • [Presentation] カイコの強力なプロモーターの同定2014

    • Author(s)
      坪田拓也, 内野恵郎, 鈴木誉保, 田中博光, 粥川琢巳, 篠田徹郎, 瀬筒秀樹
    • Organizer
      平成26年度蚕糸・昆虫機能利用学術講演会-日本蚕糸学会第84回大会
    • Place of Presentation
      日本大学生物資源科学部
    • Related Report
      2013 Annual Research Report
  • [Presentation] 血球で特異的にGAL4を発現する遺伝子組換えカイコ系統の作出2013

    • Author(s)
      坪田拓也, 内野恵郎, 神村学, 石川繭子, 浜本洋, 関水和久, 瀬筒秀樹
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2013 Annual Research Report
  • [Presentation] Transgenic Silkworm as a New BioResource2013

    • Author(s)
      Sezutsu H, Uchino K
    • Organizer
      The 5th ANRRC International Meeting
    • Place of Presentation
      Shonan Village Center, Hayama, Japan
    • Related Report
      2013 Annual Research Report
  • [Presentation] カブラハバチの遺伝子ノックアウト2013

    • Author(s)
      畠山正統, 笠嶋(炭谷)めぐみ
    • Organizer
      第34回菅平動物学セミナー
    • Place of Presentation
      筑波大菅平高原実験センター
    • Related Report
      2013 Annual Research Report
  • [Book] "Silkworm transgenesis and applications" in "Tansgenic insects"2014

    • Author(s)
      Sezutsu H, Tamura T
    • Total Pages
      398
    • Publisher
      CABI, Oxfordshire
    • Related Report
      2014 Annual Research Report
  • [Book] JATAFFジャーナル【特集】昆虫ゲノム研究と新産業への応用2014

    • Author(s)
      瀬筒秀樹
    • Total Pages
      58
    • Publisher
      公益社団法人農林水産・食品産業技術振興協会
    • Related Report
      2014 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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