Molecular and genetic approach for the drug or microbial resistance of the diamondback moth
Project/Area Number |
25292203
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Insect science
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Research Institution | National Institute of Agrobiological Sciences |
Principal Investigator |
Yamamoto Kimiko 国立研究開発法人農業生物資源研究所, 昆虫ゲノム研究ユニット, ユニット長 (40370689)
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Co-Investigator(Kenkyū-buntansha) |
MIYAMOTO KAZUHISA 農業生物資源研究所, 昆虫微生物機能研究ユニット, 上級研究員 (10370686)
WADA SANAE 農業生物資源研究所, 昆虫微生物機能研究ユニット, 主任研究員 (50414959)
JOURAKU AKIYA 農業生物資源研究所, 昆虫ゲノム研究ユニット, 主任研究員 (60542115)
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Co-Investigator(Renkei-kenkyūsha) |
NODA HIROAKI 農業生物資源研究所, 昆虫科学研究領域, 特任上級研究員 (40343991)
SATO RYOICHI 東京農工大学, 農学研究科, 教授 (30235428)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2014: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2013: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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Keywords | コナガ / ゲノム解析 / 殺虫剤抵抗性 / 連鎖地図 / 害虫防除 / ゲノム / 遺伝学 / 遺伝子 |
Outline of Final Research Achievements |
In this program, we made genome and expression gene analyses of the diamondback moth (Plutella xylostella). Finally we got a lot of fruitful results as below about drug-resistance genes of the moth. Drug-resistant strains of the diamondback moth were established.The KONAGAbase, a genomic database of the moth, was improved using the additional genomic data of the moth creating by the next-generation sequencing. The linkage analyses of the drug-resistant strains by RAD-Seq analyses strongly suggested that the ABCC2 gene of the moth had a critical role for the Cry1Ac toxin resistance. A point mutation G4946E in the ryanodine receptor gene was concluded the trigger of the flubendiamide resistance in the moth both from Japan and Thailand. As a result of the trial experiments to create fungus-resistant strains of the moth, the sensitivity differences for the entomopathogenic fungus B. bassiana were found between several diamondback moth strains.
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Report
(4 results)
Research Products
(5 results)
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[Presentation] High degree of resistance to Cry1Ab toxin caused by a single amino acid insertion in an ABC transporter in the silkworm.2013
Author(s)
Atsumi, S., Miyamoto, K., Yamamoto, K., Sezutsu, H., Wada, S., Marian R. Goldsmith, M. R., Sato, R. and Noda, H.
Organizer
Entomology 2013, 61st Annual Meeting of the Entomological Society of America
Place of Presentation
Austin, Texas, USA
Related Report
Invited
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