| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Outline of Final Research Achievements |
Our novel cryo-microscopy technique enabled cryo-observation at higher resolutions than ever. We used this technique to observe the dynamics of the endoplasmic reticulum (ER) under freezing in 4 species of woody plants and 7 species of herbaceous plants. In all plants, it was observed that the extracellular freezing induced the cessation of the streaming of ER. In Arabidopsis thaliana, furthermore, we observed the cryo-dynamics of the vacuolar membrane, the Golgi apparatus, the chloroplast and the actin filament, and all of their streamings were stopped by freezing. It was also observed that the intracellular calcium concentration was maintained high during freezing. Taken together, it is possible that plant cells which survive under freezing forcibly reduce their activity using calcium as a signal of extracelluar freezing.
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