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Symbiotic bacteria-mediated insecticide resistance: ecology of fenitrothion degrading bacteria in agricultural field

Research Project

Project/Area Number 25292208
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Environmental agriculture(including landscape science)
Research InstitutionNational Institute for Agro-Environmental Sciences

Principal Investigator

Hayatsu Masahito  国立研究開発法人 農業環境技術研究所, その他部局等, 研究員 (70283348)

Co-Investigator(Kenkyū-buntansha) TAGO Kanako  国立研究開発法人農業環境技術研究所, 生物生態機能研究領域, 主任研究員 (20432198)
Co-Investigator(Renkei-kenkyūsha) KIKUCHI Yoshitomo  国立研究開発法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (30571864)
HORI Tomoyuki  国立研究開発法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (20509533)
ISHIZAKA Masumi  国立研究開発法人農業環境技術研究所, 有機化学物質研究領域, 主任研究員 (60354007)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2013: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywordsカメムシ / フェニトロチオン / 農薬分解 / 殺虫剤抵抗性 / 農薬抵抗性 / 土壌 / 農薬耐性 / 有機リン系殺虫剤
Outline of Final Research Achievements

Several Burkholderia strains which are capable of degrading an insecticide, fenitrothion, can establish specific and beneficial symbiosis with some genera of stinkbugs; thereby, making their host insects conferring fenitrothion-resistance to the host insects. The objective of this study was to understand the ecology of Burkholderia symbiotic degraders in a free-living environment.
A field research was conducted in farmer’s fields where stinkbugs (Cavelerius sp.) harboring degraders of Burkholderia species have been identified. The research confirmed that both plant and rhizosphere constitute the environmental reservoirs for stinkbug symbiotic degraders.
Repeated applications of fenitrothion to soil can increase the numbers of degraders. The microcosm experiment with mathematical modeling showed the number of dominant species among the degraders declined with its density increase by fenitrothion applications, which can be explained according to the competitive exclusion principle.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (12 results)

All 2015 2014 2013

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 1 results,  Open Access: 3 results) Presentation (7 results)

  • [Journal Article] Insecticide applications to soil contribute to the development of Burkholderia mediating insecticide resistance in stinkbugs2015

    • Author(s)
      Kanako Tago, Yoshitomo Kikuchi, Sinji Nakaoka, Chie Katsuyama, Masahito Hayatsu
    • Journal Title

      Molecular ecology

      Volume: 24 Issue: 14 Pages: 3766-3778

    • DOI

      10.1111/mec.13265

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 害虫に農薬抵抗性を付与する土壌微生物2015

    • Author(s)
      多胡香奈子
    • Journal Title

      日本土壌肥料学雑誌

      Volume: 87 Pages: 140-145

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Insecticide-Degrading <i>Burkholderia</i> Symbionts of the Stinkbug Naturally Occupy Various Environments of Sugarcane Fields in a Southeast Island of Japan2015

    • Author(s)
      Kanako Tago, Takashi Okubo, Hideomi Itoh, Yoshitomo Kikuchi, Tomoyuki Hori, Yuya Sato, Atsushi Nagayama, Kentaro Hayashi, Seishi Ikeda, Masahito Hayatsu
    • Journal Title

      Microbes and Environments

      Volume: 30 Issue: 1 Pages: 29-36

    • DOI

      10.1264/jsme2.ME14124

    • NAID

      130005061723

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A Fine-Scale Phylogenetic Analysis of Free-Living <i>Burkholderia</i> Species in Sugarcane Field Soil2014

    • Author(s)
      Kanako Tago, Hideomi Itoh, Yoshitomo Kikuchi, Tomoyuki Hori, Yuya Sato, Atsushi Nagayama, Takashi Okubo, Ronald Navarro, Tomo Aoyagi, Kentaro Hayashi, Masahito Hayatsu
    • Journal Title

      Microbes and Environments

      Volume: 29 Issue: 4 Pages: 434-437

    • DOI

      10.1264/jsme2.ME14122

    • NAID

      130004703873

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bacterial population succession and adaptation affected by insecticide application and soil spraying history2014

    • Author(s)
      Itoh H, Navarro R, Takeshita K, Tago K, Hayatsu M, Hori T, *Kikuchi Y
    • Journal Title

      Front Microbiol

      Volume: 5 Pages: 457-457

    • DOI

      10.3389/fmicb.2014.00457

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 農地から分離した有機リン系農薬分解細菌の代謝に関する特徴と害虫への感染の可能性2015

    • Author(s)
      多胡香奈子, 菊池義智, 早津雅仁
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      京都大学
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] カメムシに農薬抵抗性を与えるBurkholderia 属分解菌の土壌における生態2014

    • Author(s)
      多胡香奈子、伊藤英臣、大久保卓、菊池義智、堀知行、佐藤裕也、永山敦士、Ronald Navarro、青柳智、早津雅仁
    • Organizer
      環境微生物生態学系学会合同大会2014
    • Place of Presentation
      アクトシティ―浜松
    • Year and Date
      2014-10-21 – 2014-10-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] カメムシに農薬抵抗性を与える有機リン系農薬分解菌Burkholderia 属の多様性と生態2014

    • Author(s)
      多胡香奈子、菊池義智、伊藤英臣、堀知行、佐藤裕也、永山敦士、中岡慎二、勝山千恵、早津雅仁
    • Organizer
      日本土壌肥料学会2014年度東京大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2014-09-08 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] Insecticide applications to soil contribute to development of Burkholderia mediating insecticide resistance in pest stinkbug2014

    • Author(s)
      Tago, K., Y. Kikuchi, S. Nakaoka, C. Katsuyama, M. Hayatsu
    • Organizer
      15th International Symposium on Microbial Ecology
    • Place of Presentation
      韓国、ソウル
    • Year and Date
      2014-08-24 – 2014-08-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] Selective transmission of insecticide-degrading bacteria from ambient soils to the bean bug Riptortus pedestris2014

    • Author(s)
      Itoh, H., T. Aoyagi, R. Navarro, K. Takeshita, Y. Sato, K. Tago, M. Hayatsu, T. Hori, Y. Kikuchi
    • Organizer
      15th International Symposium on Microbial Ecology
    • Place of Presentation
      韓国、ソウル
    • Year and Date
      2014-08-24 – 2014-08-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] 害虫カメムシに殺虫剤抵抗性を与えるBurkholderia属分解菌の農地土壌における生態2013

    • Author(s)
      早津雅仁, 多胡香奈子, 菊池義智, 堀智行, 永山敦士
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      名古屋大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] カメムシに農薬抵抗性を与える有機リン系農薬分解菌の農薬散布土壌における多様性変動プロセスの解明2013

    • Author(s)
      多胡香奈子、菊池義智、勝山千恵、早津雅仁
    • Organizer
      日本微生物生態学会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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