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Development of saline and alkaline tolerant novel crops with symbiotic N fixing ability using adaptation genes from wild Vigna genetic resources

Research Project

Project/Area Number 25292209
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Environmental agriculture(including landscape science)
Research InstitutionNational Institute of Agrobiological Sciences

Principal Investigator

Tomooka Norihiko  国立研究開発法人農業生物資源研究所, 多様性活用研究ユニット, ユニット長 (40373253)

Co-Investigator(Kenkyū-buntansha) YOKOYAMA Tadashi  東京農工大学, (連合)農学研究科(研究院), 教授 (70313286)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywords野生種 / 耐塩性 / 耐アルカリ性 / 根粒菌 / 共生 / 窒素固定 / 野生マメ科植物 / 共生窒素固定 / アルカリ耐性 / 野生植物
Outline of Final Research Achievements

To develop new crop which can tolerate high level of salt and alkaline salt stress, we investigated salt stress responses of beach cowpea (Vigna marina) and symbiotic bacteria (Sinohizobium sp.) under salt conditions.
Beach cowpea which adapted to the coral beach habitat of Yaeyama islands, Okinawa, Japan, was revealed to have ability to grow under 400mM NaCl and pH9.5 conditions. Nitrogen fixing symbiotic bacteria isolated from roots of beach cowpea could tolerate up to 5% NaCl and pH11 conditions. However, nodulation process was severely damaged probably because of inhibition of root hair curling under 80mM NaCl condition. In contrast, once nodules were successfully formed, N fixing ability of beach cowpea was maintained at 60-70% of that of control (no NaCl condition) even at 7 days after 400mM NaCl treatment. On the contrary, soybean (cv. ‘Enrei’) nodulated with B. japonicum (USDA110) died within 7 days under 400mM NaCl condition.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (16 results)

All 2016 2015 2014

All Journal Article (14 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 14 results,  Open Access: 13 results,  Acknowledgement Compliant: 14 results) Presentation (2 results)

  • [Journal Article] Growth rate and gene expression of a mutant of a TetR family transcriptional regulator gene (blr7984) in Bradyrhizobium diazoefficiens USDA1102016

    • Author(s)
      Naoko Ohkama-Ohtsu, Haruna Honma, Mariko Nakagome, Maki Nagata, Hiroko Yamaya-Ito, Yoshiaki Sano, Norina Hiraoka, Takaaki Ikemi, Akihiro Suzuki, Shin Okazaki, Kiwamu Minamisawa and Tadashi Yokoyama
    • Journal Title

      Microbes Environ

      Volume: 0 Pages: 0-0

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Genotypic difference in (137) Cs accumulation and transfer from the contaminated field in Fukushima to azuki bean (Vigna angularis).2016

    • Author(s)
      Win K.T., Oo A.Z., Kojima K., Salem D., Yamaya H., Bellingrath-Kimura S.D., Tomooka N., Kaga A., Ohkama-Ohtsu N., Yokoyama T.
    • Journal Title

      J Environ Radioact

      Volume: 159 Pages: 138-147

    • DOI

      10.1016/j.jenvrad.2016.04.011

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Genetic Diversity and Symbiotic Phenotype of Hairy Vetch Rhizobia in Japan2016

    • Author(s)
      KunYuan, HiroKi Miwa, MaKi IizuKa, Tadashi Yokoyama, Yoshiharu Fujii, and Shin Okazaki
    • Journal Title

      Microbes and Environments

      Volume: 31 Issue: 2 Pages: 121-126

    • DOI

      10.1264/jsme2.ME15184

    • NAID

      130005158497

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Varietal differences in growth and Cs allocation of blackgram (Vigna mungo) under water stress2015

    • Author(s)
      Win K.T., Oo A.Z., Aung H.P., Terasaki A., Yokoyama T., Bellingrath-Kimura S.D.
    • Journal Title

      Environ. Exp. Botany

      Volume: 109 Pages: 244-253

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Peribacteroid solution of soybean root nodules partly induces genomic loci for differentiation into bacteroids of free-living Bradyrhizobium japonicum cells2015

    • Author(s)
      Ohkama-Ohtsu N., Ichida S., Yamaya H., Ohwada T., Itakura M., Hara Y., Mitsui H., Kaneko T., Tabata S., Tejima K., Saeki K., Omori H., Hayashi M., Maekawa M., Murooka Y., Tajima S., Simomura S., Nomura M., Uchiumi T., Suzuki A., Shimoda Y., Abe M., Minamisawa K., Arima Y., Yokoyama T.
    • Journal Title

      Soil Sci. Plant Nutr.,

      Volume: 61, Pages: 461-470

    • NAID

      110010031454

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Novel genetic resources in the genus Vigna unveiled from gene bank accessions2015

    • Author(s)
      Takahashi Y, Somta P, Muto C, Iseki K, Naito K, Pandiyan M, Natesan S, Tomooka N
    • Journal Title

      Plos ONE

      Volume: 0 Pages: 0-0

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The Vigna Genome Server, 'VigGS': A genomic knowledge base of the genus Vigna based on high-quality, annotated genome sequence of the azuki bean, Vigna angularis (Willd.) Ohwi & Ohashi.2015

    • Author(s)
      Sakai H, Naito K, Takahashi Y, Sato T, Yamamoto T, Muto I, Itoh T, Tomooka N
    • Journal Title

      Plant and Cell Physiology.

      Volume: 0 Issue: 1 Pages: 0-0

    • DOI

      10.1093/pcp/pcv189

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A Homoploid Hybrid Between Wild Vigna Species Found in a Limestone Karst2015

    • Author(s)
      Takahashi Y, Iseki K, Kitazawa K, Muto C, Somta P, Irie K, Naito K, Tomooka N
    • Journal Title

      Front Plant Sci

      Volume: 6 Pages: 1050-1050

    • DOI

      10.3389/fpls.2015.01050

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Construction of an SSR and RAD-Marker Based Molecular Linkage Map of Vigna vexillata (L.) A. Rich2015

    • Author(s)
      Marubodee R, Ogiso-Tanaka E, Isemura T, Chankaew S, Kaga A, Naito K, Ehara H, Tomooka N
    • Journal Title

      Plos ONE

      Volume: 0 Issue: 9 Pages: 0-0

    • DOI

      10.1371/journal.pone.0138942

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The power of single molecule real-time sequencing technology in the de novo assembly of a eukaryotic genome2015

    • Author(s)
      Sakai H, Naito K, Ogiso-Tanaka E, Takahashi Y, Iseki K, Muto C, Satou K, Teruya K, Shiroma A, Shimoji M, Hirano T, Itoh T, Kaga A, Tomooka N
    • Journal Title

      Scientific Reports

      Volume: 5 Issue: 1 Pages: 0-0

    • DOI

      10.1038/srep16780

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Salt tolerance in wild relatives of adzuki bean, Vigna angularis (Willd.) Ohwi & Ohashi2015

    • Author(s)
      Yoshida Y., Marubodee R., Ogiso-Tanaka E., Iseki K., Isemura T., Takahashi Y., Muto C., Naito K., Kaga A., Okuno K., Ehara H., Tomooka N
    • Journal Title

      Genetic Resources and Crop Evolution

      Volume: 0 Issue: 4 Pages: 0-0

    • DOI

      10.1007/s10722-015-0272-0

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Reciprocal translocation identified in Vigna angularis dominates the wild population in East Japan2015

    • Author(s)
      Wang L, Kikuchi S, Muto C, Naito K, Isemura T, Ishimoto M, Cheng X, Kaga A, Tomooka N
    • Journal Title

      Journal of Plant Research

      Volume: 0 Issue: 4 Pages: 0-0

    • DOI

      10.1007/s10265-015-0720-0

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Peribacteroid solution of soybean root nodules partly induces genomic loci for differentiation into bacteroids of free-living Bradyrhizobium japonicum cells2015

    • Author(s)
      Ohkama-Ohtsu N., Ichida S., Yamaya H., Ohwada T., Itakura M., Hara Y., Mitsui H., Kaneko T., Tabata S., Tejima K., Saeki K., Omori H., Hayashi M., Maekawa M., Murooka Y., Tajima S., Simomura S., Nomura M., Uchiumi T., Suzuki A., Shimoda Y., Abe M., Minamisawa K., Arima Y., Yokoyama T.(責任著者)
    • Journal Title

      Soil Sci. Plant Nutr.

      Volume: 60 Issue: 3 Pages: 461-470

    • DOI

      10.1080/00380768.2014.994470

    • NAID

      110010031454

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] QTL mapping for salt tolerance and domestication-related traits in Vigna marina subsp. oblonga, a halophytic species2014

    • Author(s)
      Chankaew S, Somta P, Isemura T, Tomooka N, Kaga A, Vaughan DA, Srinives P
    • Journal Title

      Theoretical and Applied Genetics

      Volume: 127 Issue: 3 Pages: 691-702

    • DOI

      10.1007/s00122-013-2251-1

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Vigna属野生植物の環境耐性型新作物の開発に向けた根粒菌のイオンビーム照射による改良2015

    • Author(s)
      丸山雄大¹・武田喜代子¹・友岡憲彦²・横山正³ ・佐藤勝也⁴・大野豊
    • Organizer
      第10回高崎量子応用研究シンポジウム
    • Place of Presentation
      国立研究開発法人日本原子力研究開発機構 高崎量子応用研究所
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] 南西諸島の海浜に分布するハマアズキと耐塩性Sinorhizobium属根粒菌の共生成立を許容するNaCl濃度について2015

    • Author(s)
      丸山雄大、友岡憲彦、内藤 健、大津直子、横山 正
    • Organizer
      日本土壌微生物学会
    • Place of Presentation
      つくば市
    • Year and Date
      2015-05-22
    • Related Report
      2014 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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