Development of saline and alkaline tolerant novel crops with symbiotic N fixing ability using adaptation genes from wild Vigna genetic resources

• Project/Area Number: 25292209
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Environmental agriculture(including landscape science)
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: Tomooka Norihiko 国立研究開発法人農業生物資源研究所, 多様性活用研究ユニット, ユニット長 (40373253)
• Co-Investigator(Kenkyū-buntansha): YOKOYAMA Tadashi 東京農工大学, (連合)農学研究科(研究院), 教授 (70313286)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: 野生種 / 耐塩性 / 耐アルカリ性 / 根粒菌 / 共生 / 窒素固定 / 野生マメ科植物 / 共生窒素固定 / アルカリ耐性 / 野生植物

Outline of Final Research Achievements

To develop new crop which can tolerate high level of salt and alkaline salt stress, we investigated salt stress responses of beach cowpea (Vigna marina) and symbiotic bacteria (Sinohizobium sp.) under salt conditions.
Beach cowpea which adapted to the coral beach habitat of Yaeyama islands, Okinawa, Japan, was revealed to have ability to grow under 400mM NaCl and pH9.5 conditions. Nitrogen fixing symbiotic bacteria isolated from roots of beach cowpea could tolerate up to 5% NaCl and pH11 conditions. However, nodulation process was severely damaged probably because of inhibition of root hair curling under 80mM NaCl condition. In contrast, once nodules were successfully formed, N fixing ability of beach cowpea was maintained at 60-70% of that of control (no NaCl condition) even at 7 days after 400mM NaCl treatment. On the contrary, soybean (cv. ‘Enrei’) nodulated with B. japonicum (USDA110) died within 7 days under 400mM NaCl condition.

Research Products

• [Journal Article] Growth rate and gene expression of a mutant of a TetR family transcriptional regulator gene (blr7984) in Bradyrhizobium diazoefficiens USDA110 (2016)
  • Author(s): Naoko Ohkama-Ohtsu, Haruna Honma, Mariko Nakagome, Maki Nagata, Hiroko Yamaya-Ito, Yoshiaki Sano, Norina Hiraoka, Takaaki Ikemi, Akihiro Suzuki, Shin Okazaki, Kiwamu Minamisawa and Tadashi Yokoyama
  • Journal Title: Microbes Environ Volume: 0 Pages: 0-0
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Genotypic difference in (137) Cs accumulation and transfer from the contaminated field in Fukushima to azuki bean (Vigna angularis). (2016)
  • Author(s): Win K.T., Oo A.Z., Kojima K., Salem D., Yamaya H., Bellingrath-Kimura S.D., Tomooka N., Kaga A., Ohkama-Ohtsu N., Yokoyama T.
  • Journal Title: J Environ Radioact Volume: 159 Pages: 138-147
  • DOI: 10.1016/j.jenvrad.2016.04.011
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Genetic Diversity and Symbiotic Phenotype of Hairy Vetch Rhizobia in Japan (2016)
  • Author(s): KunYuan, HiroKi Miwa, MaKi IizuKa, Tadashi Yokoyama, Yoshiharu Fujii, and Shin Okazaki
  • Journal Title: Microbes and Environments Volume: 31 Issue: 2 Pages: 121-126
  • DOI: 10.1264/jsme2.ME15184
  • NAID: 130005158497
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Varietal differences in growth and Cs allocation of blackgram (Vigna mungo) under water stress (2015)
  • Author(s): Win K.T., Oo A.Z., Aung H.P., Terasaki A., Yokoyama T., Bellingrath-Kimura S.D.
  • Journal Title: Environ. Exp. Botany Volume: 109 Pages: 244-253
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Peribacteroid solution of soybean root nodules partly induces genomic loci for differentiation into bacteroids of free-living Bradyrhizobium japonicum cells (2015)
  • Author(s): Ohkama-Ohtsu N., Ichida S., Yamaya H., Ohwada T., Itakura M., Hara Y., Mitsui H., Kaneko T., Tabata S., Tejima K., Saeki K., Omori H., Hayashi M., Maekawa M., Murooka Y., Tajima S., Simomura S., Nomura M., Uchiumi T., Suzuki A., Shimoda Y., Abe M., Minamisawa K., Arima Y., Yokoyama T.
  • Journal Title: Soil Sci. Plant Nutr., Volume: 61, Pages: 461-470
  • NAID: 110010031454
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Novel genetic resources in the genus Vigna unveiled from gene bank accessions (2015)
  • Author(s): Takahashi Y, Somta P, Muto C, Iseki K, Naito K, Pandiyan M, Natesan S, Tomooka N
  • Journal Title: Plos ONE Volume: 0 Pages: 0-0
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The Vigna Genome Server, 'VigGS': A genomic knowledge base of the genus Vigna based on high-quality, annotated genome sequence of the azuki bean, Vigna angularis (Willd.) Ohwi & Ohashi. (2015)
  • Author(s): Sakai H, Naito K, Takahashi Y, Sato T, Yamamoto T, Muto I, Itoh T, Tomooka N
  • Journal Title: Plant and Cell Physiology. Volume: 0 Issue: 1 Pages: 0-0
  • DOI: 10.1093/pcp/pcv189
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A Homoploid Hybrid Between Wild Vigna Species Found in a Limestone Karst (2015)
  • Author(s): Takahashi Y, Iseki K, Kitazawa K, Muto C, Somta P, Irie K, Naito K, Tomooka N
  • Journal Title: Front Plant Sci Volume: 6 Pages: 1050-1050
  • DOI: 10.3389/fpls.2015.01050
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Construction of an SSR and RAD-Marker Based Molecular Linkage Map of Vigna vexillata (L.) A. Rich (2015)
  • Author(s): Marubodee R, Ogiso-Tanaka E, Isemura T, Chankaew S, Kaga A, Naito K, Ehara H, Tomooka N
  • Journal Title: Plos ONE Volume: 0 Issue: 9 Pages: 0-0
  • DOI: 10.1371/journal.pone.0138942
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The power of single molecule real-time sequencing technology in the de novo assembly of a eukaryotic genome (2015)
  • Author(s): Sakai H, Naito K, Ogiso-Tanaka E, Takahashi Y, Iseki K, Muto C, Satou K, Teruya K, Shiroma A, Shimoji M, Hirano T, Itoh T, Kaga A, Tomooka N
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 0-0
  • DOI: 10.1038/srep16780
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Salt tolerance in wild relatives of adzuki bean, Vigna angularis (Willd.) Ohwi & Ohashi (2015)
  • Author(s): Yoshida Y., Marubodee R., Ogiso-Tanaka E., Iseki K., Isemura T., Takahashi Y., Muto C., Naito K., Kaga A., Okuno K., Ehara H., Tomooka N
  • Journal Title: Genetic Resources and Crop Evolution Volume: 0 Issue: 4 Pages: 0-0
  • DOI: 10.1007/s10722-015-0272-0
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Reciprocal translocation identified in Vigna angularis dominates the wild population in East Japan (2015)
  • Author(s): Wang L, Kikuchi S, Muto C, Naito K, Isemura T, Ishimoto M, Cheng X, Kaga A, Tomooka N
  • Journal Title: Journal of Plant Research Volume: 0 Issue: 4 Pages: 0-0
  • DOI: 10.1007/s10265-015-0720-0
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Peribacteroid solution of soybean root nodules partly induces genomic loci for differentiation into bacteroids of free-living Bradyrhizobium japonicum cells (2015)
  • Author(s): Ohkama-Ohtsu N., Ichida S., Yamaya H., Ohwada T., Itakura M., Hara Y., Mitsui H., Kaneko T., Tabata S., Tejima K., Saeki K., Omori H., Hayashi M., Maekawa M., Murooka Y., Tajima S., Simomura S., Nomura M., Uchiumi T., Suzuki A., Shimoda Y., Abe M., Minamisawa K., Arima Y., Yokoyama T.（責任著者）
  • Journal Title: Soil Sci. Plant Nutr. Volume: 60 Issue: 3 Pages: 461-470
  • DOI: 10.1080/00380768.2014.994470
  • NAID: 110010031454
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] QTL mapping for salt tolerance and domestication-related traits in Vigna marina subsp. oblonga, a halophytic species (2014)
  • Author(s): Chankaew S, Somta P, Isemura T, Tomooka N, Kaga A, Vaughan DA, Srinives P
  • Journal Title: Theoretical and Applied Genetics Volume: 127 Issue: 3 Pages: 691-702
  • DOI: 10.1007/s00122-013-2251-1
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Vigna属野生植物の環境耐性型新作物の開発に向けた根粒菌のイオンビーム照射による改良 (2015)
  • Author(s): 丸山雄大¹・武田喜代子¹・友岡憲彦²・横山正³ ・佐藤勝也⁴・大野豊
  • Organizer: 第10回高崎量子応用研究シンポジウム
  • Place of Presentation: 国立研究開発法人日本原子力研究開発機構 高崎量子応用研究所
  • Year and Date: 2015-10-08
• [Presentation] 南西諸島の海浜に分布するハマアズキと耐塩性Sinorhizobium属根粒菌の共生成立を許容するNaCl濃度について (2015)
  • Author(s): 丸山雄大、友岡憲彦、内藤 健、大津直子、横山 正
  • Organizer: 日本土壌微生物学会
  • Place of Presentation: つくば市
  • Year and Date: 2015-05-22