Development of a next generation-diagnostic system for allergic disorders, a component-resolved diagnosis, to create personalized vaccines for allergen-specific immunotherapy

• Project/Area Number: 25292218
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Hiroshima University
• Principal Investigator: ONO KAZUHISA 広島大学, 先端物質科学研究科, 名誉教授 (10144883)
• Co-Investigator(Kenkyū-buntansha): KAWAMOTO SEIJI 広島大学, 大学院先端物質科学研究科, 教授 (90294537) ; NAKANO MIYAKO 広島大学, 大学院先端物質科学研究科, 准教授 (40397724)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2013: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
• Keywords: ダニアレルギー / 分子診断 / 免疫療法 / 感作アレルゲン / 喘息 / アレルギー / 分子診断法

Outline of Final Research Achievements

Allergen-specific immunotherapy (AIT) is the only curative treatment for allergic disorders, but current AIT using crude allergen extract has problems such as risk for side effects and poor clinical outcomes. In this study, we aimed to develop a molecular diagnostic system of allergy for effective AIT. Here, we showed a successful and comprehensive production of 26 derivatives from 23 house dust allergens as recombinant proteins. We established the molecular diagnostic system using the recombinant allergen-molecules to elucidate individual IgE-binding allergen profiles enable us to develop a personalized AIT vaccine for individuals and predict progression of house dust mite allergy.

Research Products

• [Presentation] 組換えダニアレルゲンを用いた分子種診断によるダニ喘息の病態鑑別の試み (2016)
  • Author(s): 藤村孝志、住田絃之介、村上亮太 田中明彦、林鷹治、秋庸裕、小埜和久、河本正次
  • Organizer: 第65回日本アレルギー学会学術大会
  • Place of Presentation: 東京国際フォーラム、東京
  • Year and Date: 2016-06-17
• [Presentation] The challenge towards discrimination of clinical symptoms by component-resolved diagnosis using a repertoire of recombinant house dust mite allergens (2016)
  • Author(s): Kareem Gamal ElRamlawy, Takashi Fujimura, Gennosuke Sumida, Ryota Murakami , Akihiko Tanaka, Takaharu Hayashi, Tsunehiro Aki, Kazuhisa Ono, Seiji Kawamoto
  • Organizer: EAACI Congress ２０１６
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2016-06-11
  • Int'l Joint Research
• [Presentation] ダニアレルギーの次世代型分子診断実現に向けた基盤技術の開発 (2015)
  • Author(s): 住田絃之介、小田泰裕、Kareem Gamal El－Ramrawy、田中明彦、林鷹治、秋庸裕、小埜和久、河本正次
  • Organizer: 第67回日本生物工学会大会
  • Place of Presentation: 城山観光ホテル、鹿児島市
  • Year and Date: 2015-10-26
• [Presentation] Decreased IgE-binding capacity of fungal and pet allergens upon treatment with positive and negative cluster ions (2014)
  • Author(s): Yasuhiro Ota, Takuya Abe, Tomoaki Takado, Yoshinori Sekoguchi, Kazuo Nishikawa, Kazuhisa Ono, and Seiji Kawamoto
  • Organizer: The European Academy of Allergy and Clinical Immunology Congress 2014
  • Place of Presentation: ベラ・センター、コペンハーゲン市、デンマーク
  • Year and Date: 2014-06-07 – 2014-06-11
• [Presentation] A serine protease allergen from Japanese cedar pollen induces thymic stromal lymphopoietin (TSLP) secretion from human epithelial cells (2014)
  • Author(s): Katsuma Koyamatsu, Ahmed Ragaa Nour Ibrahim, Keisuke Mizuno, Genta Kumagai, Daisuke Nishioka, Tsunehiro Aki, and Kazuhisa Ono
  • Organizer: The European Academy of Allergy and Clinical Immunology Congress 2014
  • Place of Presentation: ベラ・センター、コペンハーゲン市、デンマーク
  • Year and Date: 2014-06-07 – 2014-06-11
• [Presentation] Component-resolved molecular diagnosis of house dust mite allergy using a comprehensive repertoire of recombinant mite allergens (2013)
  • Author(s): Kishikawa Y., Kawamoto S., Nakahara H., Watakabe Y., Ota Y., Aki T., Asaoku Y., Hayashi T., Tanaka A., Ono K.
  • Organizer: EAACI-WAO Congress 2013
  • Place of Presentation: Milan, Italy