Identification and design of PPR-based Rf protein
| Project/Area Number |
25292219
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Kyushu University |
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Principal Investigator |
Nakamura Takahiro 九州大学, (連合)農学研究科(研究院), 准教授 (10464398)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Keywords | PPR / ゲノム編集 / CMS / 細胞質雄性不稔 / ミトコンドリア / 植物 |
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| Outline of Final Research Achievements |
Biochemical and in silico analysis was applied to Rf-like PPR proteins those contain various amino acid polymorphism, to identify amino acids that connecting the functionality of Rf proteins. Further, a Rf protein recognizes RNA sequence and the subsequent structural change results in suppression of CMS gene expression of orf125 gene in ogura-CMS radish.
Custom PPR protein was designed forCMS gene suppression The custom, artificial PPR protein displayed expected RNA sequence recognition. This custom PPR technology was applied to develop a molecular tool for suppressing a CMS gene for a CMS rice.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Homologous recombination-independent large gene cassette knock-in in CHO cells using TALEN and MMEJ-directed donor plasmids.2015
Author(s)
Sakuma, T., Takenaga, M., Kawabe, Y., Nakamura, T., Kamihira, M. & Yamamoto, T.
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Journal Title
Int. J. Mol. Sci.
Volume: 16
Issue: 10
Pages: 23849-23866
DOI
Related Report
Peer Reviewed / Open Access
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