| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Outline of Final Research Achievements |
Biochemical and in silico analysis was applied to Rf-like PPR proteins those contain various amino acid polymorphism, to identify amino acids that connecting the functionality of Rf proteins. Further, a Rf protein recognizes RNA sequence and the subsequent structural change results in suppression of CMS gene expression of orf125 gene in ogura-CMS radish.
Custom PPR protein was designed forCMS gene suppression The custom, artificial PPR protein displayed expected RNA sequence recognition. This custom PPR technology was applied to develop a molecular tool for suppressing a CMS gene for a CMS rice.
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