| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
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| Outline of Final Research Achievements |
In this study, the researcher has applied a method of phosphate affinity gel electrophoresis, Phos-tag SDS-PAGE that was originally developed for the separation analysis of phosphoprotein species, to a three-dimensional electrophoresis (3-DE) procedure. The use of the procedure of Phos-tag SDS-PAGE as three-dimensional electrophoresis permitted us to analyze the post-translational modification of proteins including protein phosphorylation and ubiquitination. By coupling with fluorescence difference gel electrophoresis, furthermore, the novel 3-DE procedure permitted highly sensitive detections of phosphorylated species of multiple intracellular proteins.
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