Early diagnosis of the defect in amino acid metabolism using chiral amino acid metabolomics
| Project/Area Number |
25293007
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Kyushu University |
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Principal Investigator |
Kenji Hamase 九州大学, 薬学研究科(研究院), 准教授 (10284522)
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| Co-Investigator(Kenkyū-buntansha) |
UEDA Tadashi 九州大学, 大学院薬学研究院, 教授 (90184928)
KOYANAGI Satoru 九州大学, 薬学研究科(研究院), 准教授 (60330932)
AISO Sadakazu 慶應義塾大学, 医学部, 教授 (60138013)
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| Co-Investigator(Renkei-kenkyūsha) |
ENDO Fumio 熊本大学, 医学部, 教授 (00176801)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Keywords | 分析化学 / メタボロミクス / アミノ酸 / 光学分割 |
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| Outline of Final Research Achievements |
By using chiral amino acid analysis discriminating the enantiomers, highly sensitive and selective analytical methods have been designed and applied to various animal models. Chiral amino acid profiles sometimes drastically change in the tissues and physiological fluids under disease conditions. In the present study, various metabolic related amino acids were selected as the analytical targets, and enantioselective multi-dimensional HPLC systems have been developed for the diagnosis of the metabolic disorders of aromatic amino acids, branched aliphatic amino acids, sulfur-containing amino acids and urea cycle. As a result, target amino acid enantiomers could be determined without severe interference in the plasma and urine, and possible biomarkers were found.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Establishment of a Two-Dimensional HPLC-MS/MS Method Combined with DCl/D<sub>2</sub>O Hydrolysis for the Determination of Trace Amounts of D-Amino Acid Residues in Proteins2015
Author(s)
Shoto ISHIGO, Eiichi NEGISHI, Yurika MIYOSHI, Hirohisa ONIGAHARA, Masashi MITA, Tetsuya MIYAMOTO, Haruhiko MASAKI, Hiroshi HOMMA, Tadashi UEDA, Kenji HAMASE
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Journal Title
CHROMATOGRAPHY
Volume: 36
Issue: 2
Pages: 45-50
DOI
NAID
ISSN
1342-8284, 1348-3315
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Design and synthesis of a novel pre-column derivatization reagent with a 6-methoxy-4-quinolone moiety for fluorescence and tandem mass spectrometric detection and its application to chiral amino acid analysis2015
Author(s)
Tsubasa OYAMA, Eiichi NEGISHI, Hirohisa ONIGAHARA, Nao KUSANO, Yurika MIYOSHI, Masashi MITA, Manabu NAKAZONO, Sumio OHTSUKI, Akio OJIDA, Wolfgang LINDNER, Kenji HAMASE
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Journal Title
Journal of Pharmaceutical and Biomedical Analysis
Volume: 116
Pages: 71-79
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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