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Early diagnosis of the defect in amino acid metabolism using chiral amino acid metabolomics

Research Project

Project/Area Number 25293007
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Physical pharmacy
Research InstitutionKyushu University

Principal Investigator

Kenji Hamase  九州大学, 薬学研究科(研究院), 准教授 (10284522)

Co-Investigator(Kenkyū-buntansha) UEDA Tadashi  九州大学, 大学院薬学研究院, 教授 (90184928)
KOYANAGI Satoru  九州大学, 薬学研究科(研究院), 准教授 (60330932)
AISO Sadakazu  慶應義塾大学, 医学部, 教授 (60138013)
Co-Investigator(Renkei-kenkyūsha) ENDO Fumio  熊本大学, 医学部, 教授 (00176801)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Keywords分析化学 / メタボロミクス / アミノ酸 / 光学分割
Outline of Final Research Achievements

By using chiral amino acid analysis discriminating the enantiomers, highly sensitive and selective analytical methods have been designed and applied to various animal models. Chiral amino acid profiles sometimes drastically change in the tissues and physiological fluids under disease conditions. In the present study, various metabolic related amino acids were selected as the analytical targets, and enantioselective multi-dimensional HPLC systems have been developed for the diagnosis of the metabolic disorders of aromatic amino acids, branched aliphatic amino acids, sulfur-containing amino acids and urea cycle. As a result, target amino acid enantiomers could be determined without severe interference in the plasma and urine, and possible biomarkers were found.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (33 results)

All 2016 2015 2014 2013 Other

All Journal Article (9 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 9 results,  Open Access: 3 results,  Acknowledgement Compliant: 5 results) Presentation (23 results) (of which Int'l Joint Research: 2 results,  Invited: 20 results) Remarks (1 results)

  • [Journal Article] Enantioselective Determination of Phenylalanine, Tyrosine and 3,4-Dihydroxyphenylalanine in the Urine of D-Amino Acid Oxidase Deficient Mice Using Two-Dimensional High-Performance Liquid Chromatography2016

    • Author(s)
      Reiko KOGA, Yurika MIYOSHI, Yu SATO, Masashi MITA, Ryuichi KONNO, Wolfgang LINDNER, Kenji HAMASE
    • Journal Title

      CHROMATOGRAPHY

      Volume: 37 Issue: 1 Pages: 15-22

    • DOI

      10.15583/jpchrom.2015.040

    • NAID

      130005133691

    • ISSN
      1342-8284, 1348-3315
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Establishment of a Two-Dimensional HPLC-MS/MS Method Combined with DCl/D<sub>2</sub>O Hydrolysis for the Determination of Trace Amounts of D-Amino Acid Residues in Proteins2015

    • Author(s)
      Shoto ISHIGO, Eiichi NEGISHI, Yurika MIYOSHI, Hirohisa ONIGAHARA, Masashi MITA, Tetsuya MIYAMOTO, Haruhiko MASAKI, Hiroshi HOMMA, Tadashi UEDA, Kenji HAMASE
    • Journal Title

      CHROMATOGRAPHY

      Volume: 36 Issue: 2 Pages: 45-50

    • DOI

      10.15583/jpchrom.2015.017

    • NAID

      130005098795

    • ISSN
      1342-8284, 1348-3315
    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Changes in D-aspartic acid and D-glutamic acid levels in the tissues and physiological fluids of mice with various D-aspartate oxidase activities2015

    • Author(s)
      Hai HAN, Yurika MIYOSHI, Reiko KOGA, Masashi MITA, Ryuichi KONNO, Kenji HAMASE
    • Journal Title

      Journal of Pharmaceutical and Biomedical Analysis

      Volume: 116 Pages: 47-52

    • DOI

      10.1016/j.jpba.2015.05.013

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Design and synthesis of a novel pre-column derivatization reagent with a 6-methoxy-4-quinolone moiety for fluorescence and tandem mass spectrometric detection and its application to chiral amino acid analysis2015

    • Author(s)
      Tsubasa OYAMA, Eiichi NEGISHI, Hirohisa ONIGAHARA, Nao KUSANO, Yurika MIYOSHI, Masashi MITA, Manabu NAKAZONO, Sumio OHTSUKI, Akio OJIDA, Wolfgang LINDNER, Kenji HAMASE
    • Journal Title

      Journal of Pharmaceutical and Biomedical Analysis

      Volume: 116 Pages: 71-79

    • DOI

      10.1016/j.jpba.2015.05.039

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Chiral amino acid analysis of Japanese traditional Kurozu and the developmental changes during earthenware jar fermentation processes2014

    • Author(s)
      Yurika MIYOSHI, Masanobu NAGANO, Shoto ISHIGO, Yusuke ITO, Kazunori HASHIGUCHI, Naoto HISHIDA, Masashi MITA, Wolfgang LINDNER, Kenji HAMASE
    • Journal Title

      Journal of Chromatography B

      Volume: 966 Pages: 187-192

    • DOI

      10.1016/j.jchromb.2014.01.034

    • Related Report
      2014 Annual Research Report 2013 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] キラルアミノ酸のメタボロミクス2014

    • Author(s)
      浜瀬健司
    • Journal Title

      ファルマシア

      Volume: 50 Pages: 315-320

    • NAID

      130005154891

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enantioselective two-dimensional high-performance liquid chromatographic determination of amino acids; analysis and physiological significance of D-amino acids in mammals2014

    • Author(s)
      Yurika MIYOSHI, Tsubasa OYAMA, Yusuke ITOH, Kenji HAMASE
    • Journal Title

      Chromatography

      Volume: 35 Pages: 49-57

    • NAID

      130004684359

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ischemic acute kidney injury perturbs homeostasis of serine enantiomers in the body fluid in mice: early detection of renal dysfunction using the ratio of serine enantiomers2014

    • Author(s)
      Jumpei SASABE, Masataka SUZUKI, Yurika MIYOSHI, Yosuke TOJO, Chieko OKAMURA, Sonomi ITO, Ryuichi KONNO, Masashi MITA, Kenji HAMASE, Sadakazu AISO
    • Journal Title

      PLoS ONE

      Volume: 9 Issue: 1 Pages: e86504-e86504

    • DOI

      10.1371/journal.pone.0086504

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two-dimensional high-performance liquid chromatographic determination of day-night variation of D-alanine in mammals and factors controlling the circadian changes2013

    • Author(s)
      Sachise KARAKAWA, Yurika MIYOSHI, Ryuichi KONNO, Satoru KOYANAGI, Masashi MITA, Shigehiro OHDO, Kenji HAMASE
    • Journal Title

      Analytical and Bioanalytical Chemistry

      Volume: 405 Issue: 25 Pages: 8083-8091

    • DOI

      10.1007/s00216-013-7071-2

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] キラルアミノ酸分析が生み出す新たな世界-医療・食品・宇宙への展開-2016

    • Author(s)
      浜瀬健司
    • Organizer
      日本薬学会近畿支部平成28年新春特別講演会
    • Place of Presentation
      京都
    • Year and Date
      2016-01-08
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] キラルアミノ酸分析技術の開発と応用2015

    • Author(s)
      浜瀬健司
    • Organizer
      第45回機能性食品用ペプチド研究会
    • Place of Presentation
      大阪
    • Year and Date
      2015-09-30
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] キラルアミノ酸メタボロミクスによるバイオマーカー探索の新戦略2015

    • Author(s)
      浜瀬健司
    • Organizer
      第28回バイオメディカル分析科学シンポジウム
    • Place of Presentation
      長崎
    • Year and Date
      2015-08-21
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] キラルアミノ酸メタボロミクス技術の開発2015

    • Author(s)
      浜瀬健司
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] キラルアミノ酸網羅的定量分析システムの開発と応用2015

    • Author(s)
      浜瀬健司
    • Organizer
      日本プロテオーム学会2015年会
    • Place of Presentation
      熊本
    • Year and Date
      2015-07-23
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Chiral amino acid metabolomics for drug discovery, clinical diagnosis and designing functional foods/ beverages2015

    • Author(s)
      Kenji HAMASE, Yurika MIYOSHI, Masashi MITA, Wolfgang LINDNER
    • Organizer
      26th International Symposium on Pharmaceutical and Biomedical Analysis
    • Place of Presentation
      Tbilisi, Georgia
    • Year and Date
      2015-07-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Chiral amino acid targeted metabolomics for clinical diagnosis using enantioselective two-dimensional HPLC tandem-mass spectrometry2015

    • Author(s)
      Kenji HAMASE, Yurika MIYOSHI, Yayoi YONENAGA, Hirohisa ONIGAHARA, Yusuke ITO, Masashi MITA, Jumpei SASABE, Sadakazu AISO, Wolfgang LINDNER
    • Organizer
      42nd International Symposium on High Performance Liquid Phase Separations and Related Techniques
    • Place of Presentation
      Geneva, Switzerland
    • Year and Date
      2015-06-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 黒酢および各種発酵食品・飲料におけるキラルアミノ酸メタボローム解析2015

    • Author(s)
      浜瀬健司,三次百合香,中根舞子,三田真史,長野正信
    • Organizer
      日本黒酢研究会第2回学術研究会
    • Place of Presentation
      東京
    • Year and Date
      2015-03-20
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] タンパク質・アミノ酸分析のニューフロンティア:キラルアミノ酸メタボロミクスを可能とする二次元HPLC法の開発と創薬・診断への展開2014

    • Author(s)
      浜瀬健司
    • Organizer
      第24回WSフォーラム
    • Place of Presentation
      福岡
    • Year and Date
      2014-11-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] アミノ酸分析のニューフロンティア:キラルアミノ酸メタボロミクスを可能とする二次元HPLC法の開発と利用2014

    • Author(s)
      浜瀬健司
    • Organizer
      第56回日本放線菌学会学術講演会
    • Place of Presentation
      東京
    • Year and Date
      2014-11-07
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Chiral amino acid metabolomics for clinical diagnosis2014

    • Author(s)
      Kenji HAMASE
    • Organizer
      Molecular Chirality Asia 2014
    • Place of Presentation
      Beijing
    • Year and Date
      2014-10-29 – 2014-10-31
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Chiral amino acid metabolomics using two-dimensional HPLC for novel biomarker screening2014

    • Author(s)
      Kenji HAMASE, Yurika MIYOSHI, Jumpei SASABE, Tomonori KIMURA, Masashi MITA, Ryohei YAMAMOTO, Yoshitaka ISAKA, Sadakazu AISO, Wolfgang LINDNER
    • Organizer
      30th International Symposium on Chromatography
    • Place of Presentation
      Salzburg
    • Year and Date
      2014-09-14 – 2014-09-18
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] キラルアミノ酸メタボロミクスの展開を可能とする高感度選択的二次元HPLC法2014

    • Author(s)
      浜瀬健司
    • Organizer
      The 2nd International Conference of D-Amino Acid Research併設キラルサイエンスセミナー
    • Place of Presentation
      宇都宮
    • Year and Date
      2014-09-02 – 2014-09-05
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] キラルアミノ酸メタボロミクスを基盤とする創薬・診断と新たな機能性食品の探索2014

    • Author(s)
      浜瀬健司
    • Organizer
      日本食品科学工学会第61回大会
    • Place of Presentation
      福岡
    • Year and Date
      2014-08-28 – 2014-08-30
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] D/L-アミノ酸プロファイルが生み出す新たな美容・機能性食品の可能性2014

    • Author(s)
      浜瀬健司
    • Organizer
      第19回国際食品素材/添加物展・会議,第12回ヘルスフードエキスポ
    • Place of Presentation
      東京
    • Year and Date
      2014-05-21 – 2014-05-23
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] キラルアミノ酸メタボロミクスによる新規疾患バイオマーカーの探索2014

    • Author(s)
      浜瀬健司
    • Organizer
      ビタミンB研究委員会平成25年度シンポジウム
    • Place of Presentation
      大阪
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 哺乳類におけるキラルアミノ酸メタボロミクス -D-アミノ酸の分布と含量制御-2014

    • Author(s)
      浜瀬健司,三次百合香,三田真史,金野柳一
    • Organizer
      第87回日本薬理学会年会
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Chiral amino acid metabolome analysis for biomarker assay in clinical diagnosis using enantioselective 2D-HPLC2013

    • Author(s)
      Kenji HAMASE, Yurika MIYOSHI, Nao KUSANO, Jumpei SASABE, Yosuke TOJO, Masashi MITA, Sadakazu AISO, Wolfgang LINDNER
    • Organizer
      39th International Symposium on High-Performance Liquid Phase Separations and Related Techniques
    • Place of Presentation
      Amsterdam
    • Related Report
      2013 Annual Research Report
  • [Presentation] キラルアミノ酸メタボロミクスを利用する創薬・診断の展開2013

    • Author(s)
      浜瀬健司
    • Organizer
      日本分析化学会第62年会
    • Place of Presentation
      東大阪
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 二次元HPLCによるキラルアミノ酸メタボロミクス:分析法の開発と医療展開2013

    • Author(s)
      浜瀬健司
    • Organizer
      第61回質量分析総合討論会
    • Place of Presentation
      つくば
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] キラルアミノ酸メタボロミクスによる疾患バイオマーカー探索への新しいアプローチ2013

    • Author(s)
      浜瀬健司,三次百合香,笹部潤平,木村友則,山本陵平,三田真史,猪阪善隆,相磯貞和
    • Organizer
      第38回日本医用マススペクトル学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 定量的キラルアミノ酸メタボロミクスの推進を可能とする二次元HPLC法の開発2013

    • Author(s)
      浜瀬健司,三次百合香,三田真史,笹部潤平,相磯貞和
    • Organizer
      第8回メタボロームシンポジウム
    • Place of Presentation
      福岡
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Chiral amino acid metabolomics for the sensitive diagnosis of metabolic disorders2013

    • Author(s)
      Kenji HAMASE, Yusuke ITO, Hirohisa ONIGAHARA, Nao KUSANO, Yurika MIYOSHI, Masashi MITA, Wolfgang LINDNER
    • Organizer
      40th International Symposium on High-Performance Liquid Phase Separations and Related Techniques
    • Place of Presentation
      Hobart
    • Related Report
      2013 Annual Research Report
  • [Remarks] http://soyaku.phar.kyushu-u.ac.jp/

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

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