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Studies on topical application of BONAC nucleic with liposomal formulation for advanced clinical treatment

Research Project

Project/Area Number 25293008
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Physical pharmacy
Research InstitutionGifu Pharmaceutical University

Principal Investigator

Takeuchi Hirofumi  岐阜薬科大学, 薬学部, 教授 (50171616)

Co-Investigator(Kenkyū-buntansha) Hara Hideaki  岐阜薬科大学, 薬学部, 教授 (20381717)
Tahara Kohei  岐阜薬科大学, 薬学部, 講師 (30454325)
Onodera Risako  岐阜薬科大学, 薬学部, 助教 (60720399)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Keywordsリポソーム / 核酸医薬 / 点眼 / 網膜 / 肺疾患 / 前臨床試験 / ボナック核酸 / 経肺 / DDS
Outline of Final Research Achievements

For the purpose of design of the topical formulations of BONAC nucleic, the optimal liposomal formulation with the fluorescent substance, selected as its model, was established. By using these liposomal systems, their behavior was analyzed after pulmonary or ophthalmic administration. With the same technique, it was confirmed that surface property control with suitable polymers lead to controlling the behavior of carriers after administration. At the same time, the BONAC nucleic acid encapsulating liposomes were prepared to take essential data for connecting to the pre-clinical studies aimed at practical use, including stability data. From all these results, we have demonstrated possibilities for BONAC nucleic to be used as an effective drug both in the treatment of the posterior segment related disease in the eye with eye drops and treatment of special lung disease with the prolonged residence type liposomal formulation.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (30 results)

All 2016 2015 2014 2013

All Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (20 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results) Book (1 results)

  • [Journal Article] Topical Use of Angiopoietin-like Protein 2 RNAi-loaded Lipid Nanoparticles Suppresses Corneal Neovascularization.2016

    • Author(s)
      Taketani Y, Usui T, Toyono T, Shima N, Yokoo S, Kimakura M, Yamagami S, Ohno S, Onodera R, Tahara K, Takeuchi H, Kuroda M.
    • Journal Title

      Mol Ther Nucleic Acids.

      Volume: 5 Pages: e292-e292

    • DOI

      10.1038/mtna.2016.1

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Pulmonary liposomal formulations encapsulated procaterol hydrochloride by a remote loading method achieve sustained release and extended pharmacological effects.2016

    • Author(s)
      Tahara K, Tomida H, Ito Y, Tachikawa S, Onodera R, Tanaka H, Tozuka Y, Takeuchi H
    • Journal Title

      Int J Pharm.

      Volume: 505 Issue: 1-2 Pages: 139-146

    • DOI

      10.1016/j.ijpharm.2016.03.031

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Inhalation Properties and Stability of Nebulized Naked siRNA Solution for Pulmonary Therapy2016

    • Author(s)
      Tahara K, Hashimoto W, Takeuchi H
    • Journal Title

      Chemical and Pharmaceutical Bulletin

      Volume: 64 Issue: 1 Pages: 63-67

    • DOI

      10.1248/cpb.c15-00615

    • NAID

      130005115801

    • ISSN
      0009-2363, 1347-5223
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 非侵襲的な投与方法を可能とする微粒子DDS製剤の開発.2015

    • Author(s)
      田原耕平, 竹内洋文
    • Journal Title

      月刊ファインケミカル

      Volume: 1 Pages: 15-21

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Advanced particle design of liposomes for drug delivery in oral, pulmonary and ocular administration2015

    • Author(s)
      小野寺理沙子, 田原耕平, 竹内洋文
    • Journal Title

      Drug Delivery System

      Volume: 30 Issue: 2 Pages: 121-128

    • DOI

      10.2745/dds.30.121

    • NAID

      130005083738

    • ISSN
      0913-5006, 1881-2732
    • Related Report
      2015 Annual Research Report
    • Open Access
  • [Journal Article] Retinal drug delivery using eyedrop preparations of poly-l-lysine-modified liposomes2013

    • Author(s)
      Hitoshi Sasaki, Keiichi Karasawa, Kohei Hironaka, Kohei Tahara, Yuichi Tozuka, Hirofumi Takeuchi
    • Journal Title

      European Journal of Pharmaceutics and Biopharmaceutics

      Volume: 83 Issue: 3 Pages: 364-369

    • DOI

      10.1016/j.ejpb.2012.10.014

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Surface modification of liposomes using polymer–wheat germ agglutinin conjugates to improve the absorption of peptide drugs by pulmonary administration2013

    • Author(s)
      Mitsutaka Murata, Takashi Yonamine, Shota Tanaka, Kohei Tahara, Yuichi Tozuka, Hirofumi Takeuchi
    • Journal Title

      Journal of pharmaceutical sciences

      Volume: 102 Issue: 4 Pages: 1281-1289

    • DOI

      10.1002/jps.23463

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preparation of bromfenac-loaded liposomes modified with chitosan for ophthalmic drug delivery and evaluation of physicochemical properties and drug release profile2013

    • Author(s)
      Toshimasa Tsukamoto, Kohei Hironaka, Takuya Fujisawa, Daiki Yamaguchi, Kohei Tahara, Yuichi Tozuka, Hirofumi Takeuchi
    • Journal Title

      Asian journal of pharmaceutical sciences

      Volume: 8 Issue: 2 Pages: 104-109

    • DOI

      10.1016/j.ajps.2013.07.013

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Drug delivery to the ocular posterior segment using lipid emulsion via eye drop administration: Effect of emulsion formulations and surface modification2013

    • Author(s)
      Lin Ying, Kohei Tahara and Hirofumi Takeuchi
    • Journal Title

      International Journal Pharmaceutics

      Volume: 453 Issue: 2 Pages: 329-335

    • DOI

      10.1016/j.ijpharm.2013.06.024

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] Pulmonary Delivery of Peptide Drug Using Surface-modified Liposomes to Improve Systemic Absorption2016

    • Author(s)
      Tahara K., Murata M., Onodera R., Takeuchi H.
    • Organizer
      4th Asian Symposium on Pharmaceutical Science and Technology (ASPST)
    • Place of Presentation
      Shenyang, China
    • Year and Date
      2016-04-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 新規RNA型核酸の肺局所投与を目的とした製剤化2016

    • Author(s)
      中熊佑喜、小野寺理沙子、田原耕平、豊福秀一、竹内洋文
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      横浜市
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] リポソーム吸入製剤化を目的とした吸入特性評価に関する研究2015

    • Author(s)
      宇仁田 真秀、伊藤 洋祐、村田 光隆、小野寺 理沙子、 田原 耕平、竹内 洋文
    • Organizer
      第7回粉末吸入剤シンポジウム
    • Place of Presentation
      富山県富山市
    • Year and Date
      2015-11-30
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Inhalation properties of water-soluble drug loaded liposomes atomized by nebulizer2015

    • Author(s)
      Unida, S., Ito, Y., Onodera, R., Tahara, K., Takeuchi, H
    • Organizer
      Asian Federation for Pharmaceutical Sciences (AFPS) Conference
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2015-11-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Novel approaches for posterior segment ocular drug delivery with folate-modified liposomal formulation2015

    • Author(s)
      Hayashi, T., Onodera, R., Tahara, K., Takeuchi, H.
    • Organizer
      Asian Federation for Pharmaceutical Sciences (AFPS) Conference
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2015-11-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 網膜への薬物送達を目的としたオクタアルギニン修飾リポソーム点眼製剤の設計2015

    • Author(s)
      松浦由佳、小野寺理沙子、 田原耕平、竹内洋文
    • Organizer
      第32回製剤と粒子設計シンポジウム
    • Place of Presentation
      愛知県豊橋市
    • Year and Date
      2015-10-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] 肺疾患治療を目的とした機能性核酸封入 pH 応答性リポソームの設計2015

    • Author(s)
      藤本 真、伊藤洋佑、小野寺理沙子、田原耕平、竹内洋文
    • Organizer
      日本薬剤学会第30年会
    • Place of Presentation
      長崎ブリックホール
    • Year and Date
      2015-05-21 – 2015-05-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] 水溶性高分子封入リポソームの吸入製剤としての特性評価に関する研究2015

    • Author(s)
      宇仁田真秀、伊藤洋佑、小野寺理沙子、田原耕平、竹内洋文
    • Organizer
      日本薬学会第135年会
    • Place of Presentation
      神戸学院大学
    • Year and Date
      2015-03-25 – 2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] 薬物封入リポソームのネブライザーによる噴霧・吸入特性の評価2014

    • Author(s)
      伊藤洋佑、小野寺理沙子、田原耕平、竹内洋文
    • Organizer
      第 31 回 製剤と粒子設計シンポジウム
    • Place of Presentation
      ホテル日航福岡
    • Year and Date
      2014-10-23 – 2014-10-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] 後眼部への薬物送達を目的とした葉酸修飾リポソーム点眼製剤の設計2014

    • Author(s)
      林 寿樹、小野寺理沙子、田原耕平、竹内洋文
    • Organizer
      第52回粉体に関する討論会
    • Place of Presentation
      じばさんびる
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] ネブライザーを用いた薬物封入リポソーム吸入製剤に関する研究2014

    • Author(s)
      伊藤洋佑、小野寺理沙子、田原耕平、竹内洋文
    • Organizer
      第30回日本DDS学会学術集会
    • Place of Presentation
      慶応義塾大学 芝共立キャンパス
    • Year and Date
      2014-07-30 – 2014-07-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] 後眼部への薬物送達を目的とした葉酸修飾リポソーム点眼製剤の設計2014

    • Author(s)
      林 寿樹、小野寺理沙子、田原耕平、竹内洋文
    • Organizer
      第30回日本DDS学会学術集会
    • Place of Presentation
      慶応義塾大学 芝共立キャンパス
    • Year and Date
      2014-07-30 – 2014-07-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] 非侵襲的な投与方法を可能とする微粒子DDS 製剤の開発2014

    • Author(s)
      田原耕平
    • Organizer
      日本薬剤学会第29年会
    • Place of Presentation
      大宮ソニックシティビル
    • Year and Date
      2014-05-20 – 2014-05-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] TPGS/DSPE-PEG 混合ミセルを用いた点眼製剤の設計に関する研究2014

    • Author(s)
      英林、小野寺理沙子、田原耕平、竹内洋文
    • Organizer
      日本薬剤学会第29年会
    • Place of Presentation
      大宮ソニックシティビル
    • Year and Date
      2014-05-20 – 2014-05-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] TPGS/DSPE-PEG混合ミセルを用いた 点眼製剤の設計に関する研究2014

    • Author(s)
      Lin Ying, Kohei Tahara, Hirofumi Takeuchi
    • Organizer
      第29回日本薬剤学会年会
    • Place of Presentation
      大宮ソニックプラザ
    • Related Report
      2013 Annual Research Report
  • [Presentation] 後眼部への薬物送達を目的とした微粒子点眼製剤の設計と評価2013

    • Author(s)
      唐澤圭一、小野寺理沙子、田原耕平、竹内洋文
    • Organizer
      第30回 製剤と粒子設計シンポジウム
    • Place of Presentation
      岐阜都ホテル
    • Related Report
      2013 Annual Research Report
  • [Presentation] ネブライザーを用いたリポソーム吸入製剤に関する研究2013

    • Author(s)
      伊藤洋祐、小野寺理沙子、田原耕平、竹内洋文
    • Organizer
      日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術大会2013
    • Place of Presentation
      鈴鹿医療科学大学 白子キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] Liposomal Systems for Pulmonary and Ocular Drug Delivery2013

    • Author(s)
      Hirofumi Takeuchi
    • Organizer
      The 40th Annual Meeting & Exposition of the Controlled Release Society
    • Place of Presentation
      Hawaii Convention Center in Honolulu
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] キャリアーを活用する吸入・点眼投与製剤の設計研究2013

    • Author(s)
      田原耕平、竹内洋文
    • Organizer
      第31回物性物理化学研究会
    • Place of Presentation
      京都大学薬学部講堂
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Lipid emulsion as a new carrier for drug delivery to ocular posterior segment2013

    • Author(s)
      Lin Ying, Kohei Tahara, Hirofumi Takeuchi
    • Organizer
      The 5th Asian Arden Conference
    • Place of Presentation
      愛知学院大学薬学部
    • Related Report
      2013 Annual Research Report
  • [Book] 非経口投与製剤の開発と応用(リポソームによる薬物の後眼部送達)2013

    • Author(s)
      田原耕平, 廣中耕平, 竹内洋文
    • Total Pages
      7
    • Publisher
      シーエムシー出版
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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