Development of Functional Protein Separation and Purification System for Biomarker Discovery
| Project/Area Number |
25293009
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Keio University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
AYANO ERI 慶應義塾大学, 薬学部, 研究員 (10424102)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
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| Keywords | 機能性高分子 / タンパク分離 / バイオマーカー / RNA / 抗体医薬 |
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| Outline of Final Research Achievements |
A temperature-responsive solid-phase extraction (SPE) system with poly(N-isopropylacrylamide) (PNIPAAm)-based polymer modified silica beads was developed. Utilizing this system, lysozyme, and monoclonal antibodies can be purified from typical contaminants maintaining their physiological activities. An important feature of our system is that the interaction between targeted proteins and PNIPAAm-based polymer modified silica beads can be controlled by temperature switching with a mild aqueous eluent; purification was achieved without the loss of activity. In addition, this system is highly versatile, and a good alternative to limit purification costs. We believe that these purification methods with simple, versatile, inexpensive materials are of important value for biological research; furthermore, these methods provide an alternative to increase the purification efficiency of protocols for bio-pharmaceutical drugs, while reducing the total cost of production.
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Report
(4 results)
Research Products
(65 results)
