| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
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| Outline of Final Research Achievements |
A temperature-responsive solid-phase extraction (SPE) system with poly(N-isopropylacrylamide) (PNIPAAm)-based polymer modified silica beads was developed. Utilizing this system, lysozyme, and monoclonal antibodies can be purified from typical contaminants maintaining their physiological activities. An important feature of our system is that the interaction between targeted proteins and PNIPAAm-based polymer modified silica beads can be controlled by temperature switching with a mild aqueous eluent; purification was achieved without the loss of activity. In addition, this system is highly versatile, and a good alternative to limit purification costs. We believe that these purification methods with simple, versatile, inexpensive materials are of important value for biological research; furthermore, these methods provide an alternative to increase the purification efficiency of protocols for bio-pharmaceutical drugs, while reducing the total cost of production.
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