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Molecular dissection of organization and dynamics of membrane lipid domains

Research Project

Project/Area Number 25293015
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Biological pharmacy
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

KOBAYASHI Toshihide  国立研究開発法人理化学研究所, 佐甲細胞情報研究室, 客員主管研究員 (60162004)

Co-Investigator(Kenkyū-buntansha) 村手 源英  国立研究開発法人理化学研究所, その他部局等, 研究員 (30311369)
石塚 玲子  国立研究開発法人理化学研究所, その他部局等, 研究員 (60342747)
阿部 充宏  国立研究開発法人理化学研究所, その他部局等, 研究員 (90415068)
岸本 拓磨  国立研究開発法人理化学研究所, 小林脂質生物学研究室, 基礎科学特別研究員 (70585158)
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Keywords脂質ドメイン / 脂質ラフト / 脂質の可視化 / 脂質結合毒素 / コレステロール / スフィンゴ脂質 / 脂質の非対称性 / スフィンゴミエリン / 脂質プローブ / インフルエンザ / ラマン分光 / カベオレ / ホスホリパーゼC / 脂肪滴 / PLIN2 / Rab18 / フリーズフラクチャー / 脂質結合性毒素 / 上皮細胞 / 細胞分裂 / 形質膜
Outline of Final Research Achievements

In the biomembranes, lipids are not randomly distributed. Rather, lipids are asymmetrically distributed between outer and inner membrane bilayer. Even in one layer, lipids form domains. However, the detailed distribution of lipids in biomembranes are not yet elucidated. We have developed various protein probes that bind specific lipids or lipid domains. Using these probes in combination with state-of-the-art microscopy techniques, we clarified the detailed distribution of lipid domains, including lipid rafts, in biomembranes. Application of our strategy to various pathophysiological events such as cell division, cell adhesion and genetic defects in cholesterol and sphingolipid metabolism provided us new aspects of lipid domains.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (40 results)

All 2017 2016 2015 2014 2013 Other

All Int'l Joint Research (10 results) Journal Article (14 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 14 results,  Open Access: 6 results,  Acknowledgement Compliant: 10 results) Presentation (14 results) (of which Int'l Joint Research: 10 results,  Invited: 7 results) Remarks (2 results)

  • [Int'l Joint Research] 国立科学研究センター(CNRS)/国立衛生医学研究所(INSERM)(フランス)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] 国立化学研究所(スロベニア)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] クイーンズ大学(オーストラリア)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] 国立科学研究センター(CNRS)/国立衛生医学研究所(INSERM)(フランス)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] クイーンズランド大学(オーストラリア)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] 国立化学研究所(スロベニア)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] マギル大学(カナダ)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] 国立化学研究所/リュブリャナ大学(スロベニア)

    • Related Report
      2014 Annual Research Report
  • [Int'l Joint Research] クイーンズランド大学(オーストラリア)

    • Related Report
      2014 Annual Research Report
  • [Int'l Joint Research] ストラスブール大学/国立衛生医学研究所/国立科学センター(フランス)

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Photoswitchable phospholipid FRET acceptor: Detergent free intermembrane transfer assay of fluorescent lipid analogs.2017

    • Author(s)
      Sumi M, Makino A, Inaba T, Sako Y, Fujimori F, Greimel P, Kobayashi T
    • Journal Title

      Sci Rep

      Volume: in press

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A novel spingomyeling/cholesterol specific probe reveals the dynamics of the membrane domain during virus and in Nieman-Pick type C2016

    • Author(s)
      Makino, A., Sako, Y. et al.
    • Journal Title

      FASEB J.

      Volume: 31 Issue: 4 Pages: 1301-1322

    • DOI

      10.1096/fj.201500075r

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Detection of sphingomyelin clusters by Raman spectroscopy.2016

    • Author(s)
      Shirota K, Yagi K, Inaba T, Li PC, Murata M, Sugita Y, Kobayashi T.
    • Journal Title

      Biophys J

      Volume: 111 Issue: 5 Pages: 999-1007

    • DOI

      10.1016/j.bpj.2016.07.035

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes.2016

    • Author(s)
      Makino A, Hullin-Matsuda F, Murate M, Abe M, Tomishige N, Fukuda M, Yamashita S, Fujimoto T, Vidal H, Lagarde M, Delton I, Kobayashi T.
    • Journal Title

      Mol Biol Cell

      Volume: 27 Issue: 21 Pages: 3293-3304

    • DOI

      10.1091/mbc.e15-10-0730

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Phospholipase Cβ1 induces membrane tubulation and is involved in caveolae formation.2016

    • Author(s)
      Inaba T, Kishimoto T, Murate M, Tajima T, Sakai S, Abe M, Makino A, Tomishige N, Ishitsuka R, Ikeda Y, Takeoka S, Kobayashi T.
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 113 Issue: 28 Pages: 7834-7839

    • DOI

      10.1073/pnas.1603513113

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Detectors for evaluating the cellular landscape of sphingomyelin- and cholesterol-rich membrane domains2016

    • Author(s)
      Kishimoto T*, Ishitsuka R*, Kobayashia T. * equally contributed
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids

      Volume: in press Issue: 8 Pages: 812-829

    • DOI

      10.1016/j.bbalip.2016.03.013

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Pore-forming toxins: Properties, diversity, and uses as tools to image sphingomyelin and ceramide phosphoethanolamine.2016

    • Author(s)
      Yamaji-Hasegawa A, Hullin-Matsuda F, Greimel P, Kobayashi T.
    • Journal Title

      Biochim. Biophys Acta

      Volume: 1858 Issue: 3 Pages: 576-592

    • DOI

      10.1016/j.bbamem.2015.10.012

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Visualization of lipid membrane reorganization induced by a pore-forming toxin using high-speed atomic force microscopy2015

    • Author(s)
      Yilmaz N, Kobayashi T
    • Journal Title

      ACS Nano

      Volume: 9 Issue: 8 Pages: 7960-7967

    • DOI

      10.1021/acsnano.5b01041

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Asymmetric diacylglycerol dynamics on the cytoplasmic and luminal sides of a single endomembrane in living cells2015

    • Author(s)
      Ueda Y, Ogiso H, Sato M, Umezawa Y, Okazaki T, Kobayashi T
    • Journal Title

      Sci Rep

      Volume: 5 Issue: 1 Pages: 12960-12960

    • DOI

      10.1038/srep12960

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates2015

    • Author(s)
      Bhat HB, Ishitsuka R, Inaba T, Murate M, Abe M, Makino A, Kohyama-Koganeya A, Nagao K, Kurahashi A, Kishimoto T, Tahara M, Yamano A, Nagamune K, Hirabayashi Y, Juni N, Umeda M, Fujimori F, Nishibori K, Yamaji-Hasegawa A, Greimel P, Kobayashi T.
    • Journal Title

      FASEB J

      Volume: 29(9) Issue: 9 Pages: 3920-34

    • DOI

      10.1096/fj.15-272112

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transbilayer distribution of lipids at nano scale.2015

    • Author(s)
      Murate M, Abe M, Kasahara K, Iwabuchi K, Umeda M, Kobayashi T
    • Journal Title

      J Cell Sci

      Volume: 128 Pages: 1627-1638

    • DOI

      10.1242/jcs.163105

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Visualization of the heterogeneous membrane distribution of sphingomyelin associated with cytokinesis, cell polarity, and sphingolipidosis2015

    • Author(s)
      Makino A, Abe M, Murate M, Inaba T, Yilmaz N, Hullin-Matsuda F, Kishimoto T, Schieber NL, Taguchi T, Arai H, Anderluh G, Parton RG, Kobayashi T.
    • Journal Title

      FASEB J

      Volume: 29 Issue: 2 Pages: 477

    • DOI

      10.1096/fj.13-247585

    • URL

      https://pure.teikyo.jp/en/publications/d87dd0ab-850e-4f16-b10a-1b9c26322ec8

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Lipid compartmentalization in the endosome system2014

    • Author(s)
      Hullin-Matsuda F, Taguchi T, Greimel P, Kobayashi T.
    • Journal Title

      Sem Cell Dev Biol

      Volume: (in press) Pages: 48-56

    • DOI

      10.1016/j.semcdb.2014.04.010

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Real-Time Visualization of Assembling of a Sphingomyelin-Specific Toxin.2013

    • Author(s)
      N. Yilmaz, T. Yamada, P. Greimel, T. Uchihashi, T. Ando, T. Kobayashi
    • Journal Title

      Biophys. J.

      Volume: 105 Issue: 6 Pages: 1397-1405

    • DOI

      10.1016/j.bpj.2013.07.052

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release2016

    • Author(s)
      Makino A, Abe M, Ishitsuka R, Murate M, Kishimoto T, Sakai S, Hullin-Matsuda F, Inaba T, Greimel P, Kobayashi T
    • Organizer
      12th Gerli international lipidomics meeting
    • Place of Presentation
      Toulouse, France
    • Year and Date
      2016-10-23
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Phosphatidylglycerol analog regulates the synthesis of the endosome-specific and fusion enhancing lipid bis(monoacylglycero)phosphate2016

    • Author(s)
      Tan HH, Hulin-Matsuda F, Lim SR, Sudesh K, Gam LH, Greimel P, Kobayashi T
    • Organizer
      12th Gerli international lipidomics meeting
    • Place of Presentation
      Toulouse, France
    • Year and Date
      2016-10-23
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Transbilayer distribution of lipids in the plasma membrane revealed by immuno-electron microscopy2016

    • Author(s)
      Murate M
    • Organizer
      57th International Conference on the Bioscience of Lipids
    • Place of Presentation
      Chamonix Mont-Blanc, France
    • Year and Date
      2016-09-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Elucidation of the natural regioisomer of the endosome-specific lipid bis(monoacylglycero)phosphate2016

    • Author(s)
      Lim SR, Tan HH, Hullin-Matsuda F, Sudesh K, Gram LH, Greimel P, Kobayashi T
    • Organizer
      57th International Conference on the Bioscience of Lipids
    • Place of Presentation
      Chamonix Mont-Blanc, France
    • Year and Date
      2016-09-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Phosphatidylglycerol analog regulates the synthesis of the endosome-specific lipid bis(monoacylglycero)phosphate2016

    • Author(s)
      Tan HH, Hullin-Matsuda F, Lim SR, Sudesh K, Gram LH, Greimel P, Kobayashi T
    • Organizer
      57th International Conference on the Bioscience of Lipids
    • Place of Presentation
      Chamonix Mont-Blanc, France
    • Year and Date
      2016-09-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Phospholipase C beta 1 induces membrane tubulation and is involved in caveolae formation2016

    • Author(s)
      Inaba T, Kishimoto T, Murate M, Tajima T, Sakai S, Abe M, Makino A, Tomishige N, Ishitsuka R, Ikeda Y, Takeoka S, Kobayashi T
    • Organizer
      57th International Conference on the Bioscience of Lipids
    • Place of Presentation
      Chamonix Mont-Blanc, France
    • Year and Date
      2016-09-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Imaging lipids and lipid domains2016

    • Author(s)
      Kobayashi T
    • Organizer
      Joint meeting of the membrane sections of the French and German Biophysical Societies
    • Place of Presentation
      Bad Herrenalb, Germany
    • Year and Date
      2016-04-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cellular localization of sphingomyelin clusters2016

    • Author(s)
      Kobayashi T
    • Organizer
      Gordon Research Conference on Glycolipid and Sphingolipid Biology
    • Place of Presentation
      Lucca, Italy
    • Year and Date
      2016-03-06
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Imaging lipids and lipid domains2016

    • Author(s)
      Kobayashi T
    • Organizer
      Sphingonet’s International Colloquium
    • Place of Presentation
      Amsterdam, Netherland
    • Year and Date
      2016-01-27
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Imaging lipids and lipid domains2015

    • Author(s)
      Kobayashi T
    • Organizer
      11th GERLI Lipidomics Meeting
    • Place of Presentation
      Strasbourg France
    • Year and Date
      2015-10-25
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 膜を横切る脂質の運動、フリップフロップについて2013

    • Author(s)
      小林俊秀
    • Organizer
      第10回「生体膜・コロイド研究の最前線」
    • Place of Presentation
      東海村
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Free cholesterol loading induces the degradation of perilipin 2 and rab18-de pendent abnormal lipidation of apolipoprotein B in cultured hepatocytes2013

    • Author(s)
      Makino A, Hullin-Matsuda F, Murate M, Abe M, Fukuda M, Yamashita S, Fujimoto T, Vidal H, Lagarde M, Delton-Vandenbroucke I, Kobayashi T
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Imaging lipids and lipid domains

    • Author(s)
      Kobayashi T
    • Organizer
      2nd Mini Symposium “Protein-lipid interactions”
    • Place of Presentation
      Piran, Slovenia
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 脂質のイメージング

    • Author(s)
      小林俊秀
    • Organizer
      第1回JFAS講演会
    • Place of Presentation
      秋葉原
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Remarks]

    • URL

      http://lipidology.riken.jp/

    • Related Report
      2016 Annual Research Report
  • [Remarks]

    • URL

      http://lipidology.riken.jp/

    • Related Report
      2015 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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