| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2013: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Outline of Final Research Achievements |
Fungi living in plants or arthropods are attractive resources for our natural product exploration using chemical epigenetic method. After the isolation of various fungi from medicinal plants and arthropods, mainly insects and spiders, the chemical epigenetic method was applied to the fungi and found Graphiopsis chlorocephala (Paeonia lactiflora) and Mycosphaerella sp. (Aloe arborescens) were found to enhance the accumulation of their secondary metabolites, from which structurally diverse novel benzophenones and short branched fatty acid dimers were isolated.
Structurally diverse chaetophenols were previously isolated from Chaetomium fungi as novel natural products by using chemical epigenetic method, and simple aromatic compound was biosynthesized by a NR-PKS gene as a common intermediate of chaetophenols. Overexpression of the gene in Aspergillus oryzae and artificial chemical transformation of the aromatic compound generated various novel pseudo-natural products.
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