Discovery of Novel Natural Products from Fungi by Epigenetic Expression of Secondary Metabolite Biosynthetic Genes
Project/Area Number |
25293022
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Natural medicines
|
Research Institution | Tohoku University |
Principal Investigator |
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2013: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
|
Keywords | 糸状菌 / 未利用生合成遺伝子 / 天然物創出 / ケミカルエピジェネティクス / 二次代謝物 / エピジェネティクス / 構造多様性 / 天然物 / 未利用遺伝子 / エピジェネシス / 化学変換 / ケミカルスペース |
Outline of Final Research Achievements |
Fungi living in plants or arthropods are attractive resources for our natural product exploration using chemical epigenetic method. After the isolation of various fungi from medicinal plants and arthropods, mainly insects and spiders, the chemical epigenetic method was applied to the fungi and found Graphiopsis chlorocephala (Paeonia lactiflora) and Mycosphaerella sp. (Aloe arborescens) were found to enhance the accumulation of their secondary metabolites, from which structurally diverse novel benzophenones and short branched fatty acid dimers were isolated. Structurally diverse chaetophenols were previously isolated from Chaetomium fungi as novel natural products by using chemical epigenetic method, and simple aromatic compound was biosynthesized by a NR-PKS gene as a common intermediate of chaetophenols. Overexpression of the gene in Aspergillus oryzae and artificial chemical transformation of the aromatic compound generated various novel pseudo-natural products.
|
Report
(4 results)
Research Products
(21 results)
-
[Journal Article] Use of a Biosynthetic Intermediate to Explore the Chemical Diversity of Pseudo-natural Fungal Polyketides2015
Author(s)
T. Asai*, K. Tsukada, S. Ise, N. Shirata, M. Hashimoto, I. Fujii, K. Gomi, K. Nakagawara, E. N. Kodama, Y, Oshima
-
Journal Title
Nature Chem.
Volume: 7
Issue: 9
Pages: 737-743
DOI
Related Report
Peer Reviewed
-
-
-
[Journal Article] An epigenetic modifier enhances the production of anti-diabetic and anti-inflammatory sesquiterpenoids from Aspergillus sydowii2013
Author(s)
Y. M. Chung, C. K. Wei, D. W. Chuang, M. E. Shazly, C. T. Hsieh, T. Asai, Y. Oshima, T. J. Hsieh, T. L. Hwang, Y. C. Wu, F. R. Chang
-
Journal Title
Bioorg. Med. Chem
Volume: 21
Issue: 13
Pages: 3866-3872
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Suberoylanilide Hydroxamic Acid, a Histone Deacetylase Inhibitor, Induces the Production of Anti-inflammatory Cyclodepsipeptides from Beauveria felina2013
Author(s)
Y. M. Chung, M. E. Shazly, D. W. Chuang, T. L. Hwang, T. Asai, Y. Oshima, M. L. Ashour, Y. C. Wu, F. R. Chang
-
Journal Title
J. Nat. Prod.
Volume: 76
Issue: 7
Pages: 1260-1266
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-