| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2015: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2013: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
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| Outline of Final Research Achievements |
A caged NO molecule, Flu-DNB-DB, was developed and applied for cultured cells. When the cells loaded with Flu-DNB-DB was irradiated with near infrared femto-second pulse laser, NO release within the irradiated cells was observed. Another caged NO molecule, Rol-DNB, was developed. Rol-DNB was found to release NO upon photo irradiation at the range of 530-590 nm, yellow-green light, with spatiotemporal resolution. Rol-DNB was also found to be localized to mitochondria within the cells. It was demonstrated that NO release from Rol-DNB upon yellow-green light resulted in the enhancement of mitochondrial fragmentation depending on Drp-1 activation.
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