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Photocontrol of NO and H2S treatment as signaling molecules and biological applications

Research Project

Project/Area Number 25293028
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Drug development chemistry
Research InstitutionNagoya City University

Principal Investigator

Nakagawa Hidehiko  名古屋市立大学, 薬学研究科(研究院), 教授 (80281674)

Co-Investigator(Renkei-kenkyūsha) Ieda Naoya  名古屋市立大学, 大学院薬学研究科, 助教 (00642026)
Miyata Naoki  名古屋市立大学, 大学院薬学研究科, 特任教授 (50114674)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2015: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2013: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Keywordsケミカルバイオロジー / ケージド化合物 / 薬学 / 有機化学 / 光スイッチ
Outline of Final Research Achievements

A caged NO molecule, Flu-DNB-DB, was developed and applied for cultured cells. When the cells loaded with Flu-DNB-DB was irradiated with near infrared femto-second pulse laser, NO release within the irradiated cells was observed. Another caged NO molecule, Rol-DNB, was developed. Rol-DNB was found to release NO upon photo irradiation at the range of 530-590 nm, yellow-green light, with spatiotemporal resolution. Rol-DNB was also found to be localized to mitochondria within the cells. It was demonstrated that NO release from Rol-DNB upon yellow-green light resulted in the enhancement of mitochondrial fragmentation depending on Drp-1 activation.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (18 results)

All 2015 2014 2013 Other

All Journal Article (8 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 8 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (7 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] A double bond-conjugated dimethylnitrobenzene-type photolabile nitric oxide donor with improved two-photon cross section2015

    • Author(s)
      1.N. Ieda, K. Hishikawa, K. Eto, K. Kitamura, M. Kawaguchi, T. Suzuki, K. Fukuhara, N. Miyata, T. Furuta, J. Nabekura, H. Nakagawa
    • Journal Title

      Bioorg. Med. Chem. Lett.

      Volume: 25 Issue: 16 Pages: 3172-3175

    • DOI

      10.1016/j.bmcl.2015.05.095

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Profiling and relative quantification of multiply nitrated and oxidized fatty acids2015

    • Author(s)
      Milic I, Griesser E, Vemula V, Ieda N, Nakagawa H, Miyata N, Galano J-M, Oger C, Durand T, Fedorova F
    • Journal Title

      Anal. Bioanal. Chem.

      Volume: 407 Issue: 19 Pages: 5587-5602

    • DOI

      10.1007/s00216-015-8766-3

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Development of Photo-controllable Hydrogen Sulfide Donor Applicable in Live Cells2015

    • Author(s)
      Naoki Fukushima, Naoya Ieda, Mitsuyasu Kawaguchi, Kiyoshi Sasakura, Tetsuo Nagano, Kenjiro Hanaoka, Naoki Miyata, Hidehiko Nakagawa
    • Journal Title

      Bioorganic & Medicinal Chemistry Letters

      Volume: 25 Issue: 2 Pages: 175-178

    • DOI

      10.1016/j.bmcl.2014.11.084

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Synthesis of a photocontrollable hydrogen sulfide donor using ketoprofenate photocages2014

    • Author(s)
      Naoki Fukushima, Hidehiko Nakagawa, et al.
    • Journal Title

      Chem. Commun

      Volume: 50 Issue: 5 Pages: 587-589

    • DOI

      10.1039/c3cc47421f

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] hotomanipulation of Vasodilation with a Blue-Light-Controllable Nitric Oxide Releaser2014

    • Author(s)
      Naoya Ieda, Hidehiko Nakagawa, et al.
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 136 Issue: 19 Pages: 7085-7091

    • DOI

      10.1021/ja5020053

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Visible light-induced nitric oxide release from a novel nitrobenzene derivative cross-conjugated with a coumarin fluorophore2014

    • Author(s)
      Kai Kitamura, Hidehiko Nakagawa et al.
    • Journal Title

      Bioorg. Med. Chem. Lett.

      Volume: 24 Issue: 24 Pages: 5660-5662

    • DOI

      10.1016/j.bmcl.2014.10.075

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Fine spatiotemporal control of nitric oxide release by infrared pulse-laser irradiation of a photo-labile donor2013

    • Author(s)
      Hidehiko Nakagawa, Kazuhiro Hishikawa, Kei Eto, et al.
    • Journal Title

      ACS. Chem. Biol.

      Volume: 8 Issue: 11 Pages: 2493-2500

    • DOI

      10.1021/cb400361m

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Reductant-Resistant and Metal-Free Fluorescent Probe for Nitroxyl Applicable to Living Cells2013

    • Author(s)
      Kodai Kawai, Naoya Ieda, Hidehiko Nakagawa, et al.
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 135 Issue: 34 Pages: 12690-12696

    • DOI

      10.1021/ja404757s

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] Two-photon uncaging of photo-NO releasers and vasodilation in living mouse brain2015

    • Author(s)
      Hidehiko Nakagawa
    • Organizer
      Pacifichem2015
    • Place of Presentation
      Honolulu, HI, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Development of Blue-Light-Controllable Nitric Oxide Releaser and its Biological Application2015

    • Author(s)
      Naoya Ieda, Hidehiko Nakagawa, et al.
    • Organizer
      SFRBM2015
    • Place of Presentation
      Boston, MA, USA
    • Year and Date
      2015-11-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 光で制御する一酸化窒素・活性酸素投与法の開発と応用2015

    • Author(s)
      中川秀彦
    • Organizer
      第68回日本酸化ストレス学会学術集会
    • Place of Presentation
      鹿児島
    • Year and Date
      2015-06-11
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Caged compounds for gaseous mediators, nitric oxide and hydrogen sulfide, and their biological applications2015

    • Author(s)
      Hidehiko Nakagawa
    • Organizer
      3rd Bioscience and Biotechnology International Symposium
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      2015-01-14
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Mitochondria-targeting Nitric Oxide Releasers Controllable with Visible Light2014

    • Author(s)
      Kai Kitamura, Hidehiko Nakagawa, et al.
    • Organizer
      ICBS2014
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      2014-11-17 – 2014-11-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Photo-inducible hydrogen sulfide releaser using ketoprofenate photocages2014

    • Author(s)
      Hidehiko Nakagawa, et al.
    • Organizer
      Third International Conference on H2S Biology and Medicine
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2014-06-04 – 2014-06-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] 光解除性保護基を用いた光制御型硫化水素供与化合物の合成と機能評価2014

    • Author(s)
      福島直樹、中川秀彦ほか
    • Organizer
      日本薬学会第134年会
    • Place of Presentation
      熊本
    • Related Report
      2013 Annual Research Report
  • [Remarks] 名古屋市立大学薬学部・大学院薬学研究科薬化学分野

    • URL

      http://www.phar.nagoya-cu.ac.jp/hp/ykg/Yakka/index.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 名古屋市立大学大学院薬学研究科薬化学分野

    • URL

      http://www.phar.nagoya-cu.ac.jp/hp/ykg/Yakka/index.html

    • Related Report
      2014 Annual Research Report
  • [Patent(Industrial Property Rights)] N-ニトロソアニリン誘導体、並びに、それを用いたNO発生剤及びNOの発生方法2013

    • Inventor(s)
      家田直弥、中川秀彦
    • Industrial Property Rights Holder
      名古屋市立大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-234477
    • Filing Date
      2013-11-12
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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