Cellular and molecular studies on expression of sperm functional proteins relating with infertility using gene-modified mouse lines
| Project/Area Number |
25293041
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Chiba University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
Ito Chizuru 千葉大学, 大学院医学研究院, 講師 (80347054)
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| Co-Investigator(Renkei-kenkyūsha) |
Maekawa Mamiko 千葉大学, 大学院医学研究院, 助教 (20181571)
Kamimura Kyouko 千葉大学, 大学院医学研究院, 技術専門職員 (20422264)
Mutoh Tohru 千葉大学, 大学院医学研究院, 技術専門職員 (30422265)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2013: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
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| Keywords | 精子 / 遺伝子改変マウス / 受精 / 不妊 / エクアトリン / equatorin / 不妊症 / Equatorin / 授精 / 卵子 |
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| Outline of Final Research Achievements |
Using the established Eqtn-Tg(EGFP) and Eqtn-KO mouse lines, we analyzed live-images of the acrosome region that is the sperm fusion site for egg and molecular change from the acrosome reaction throughout to egg activation. A whole cell imaging (80-100nm acrosomal membrane complex) of sperm was obtained by new microscopy STED system without sectioning, which suggested a possibility of live cell imaging during fertilization (Microscopy, 2015). We clarified the distribution abnormality of gamete fusion-related spesp1 in the Eqtn-knockout (KO) mouse line, fertility loss of the Eqtn/Spesp1 double KO mouse line and relations with other gamete-fusion related proteins. Based on these analyses, we proposed the novel mechanism of sperm membrane modifications leading to gamete fusion in mammals including humans. Manuscripts showing these events are prepared for publication.
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Report
(4 results)
Research Products
(58 results)
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[Journal Article] A mutation of COX6A1 causes a recessive axonal or mixed form of Charcot-Marie-Tooth disease.2014
Author(s)
Tamiya G, Makino S, Hayashi M, Abe A, Numakura C, Ueki M, Tanaka A, Ito C, Toshimori K, Ogawa N, Terashima T, Maegawa H, Yanagisawa D, Tooyama I, Tada M, Onodera O, Hayasaka K.
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Journal Title
Am J Hum Genet.
Volume: 95
Issue: 3
Pages: 294-300
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A heterozygous mutation of GALNTL5 affects male infertility with impairment of sperm motility2014
Author(s)
Takasaki N, Tachibana K, Ogasawara S, Matsuzaki H, Hagiuda J, Ishikawa H, Mochida K, Inoue K, Ogonuki N, Ogura A, Noce T, Ito C, Toshimori K, Narimatsu H.
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Journal Title
Proc Natl Acad Sci USA
Volume: 111
Issue: 3
Pages: 1120-1125
DOI
Related Report
Peer Reviewed
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[Journal Article] Oligo-astheno-teratozoospermia in mice lacking ORP4, a sterol-binding protein in the OSBP-related protein family.2014
Author(s)
Udagawa O, Ito C, Ogonuki N, Sato H, Lee S, Tripvanuntakul P, Ichi I, Uchida Y, Nishimura T, Murakami M, Ogura A, Inoue T, Toshimori K, Arai H.
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Journal Title
Genes Cells.
Volume: 19
Issue: 1
Pages: 13-27
DOI
Related Report
Peer Reviewed
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[Journal Article] The Arf GAP SMAP2 is necessary for organized vesicle budding from the trans-Golgi network and subsequent acrosome formation in spermiogenesis.2013
Author(s)
Funaki T, Kon S, Tanabe K, Natsume W, Sato S, Shimizu T, Yoshida N, Wong WF, Ogura A, Ogawa T, Inoue K, Ogonuki N, Miki H, Mochida K, Endoh K, Yomogida K, Fukumoto M, Horai R, Iwakura Y, Ito C, Toshimori K, Watanabe T, Satake M.
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Journal Title
Mol. Biol. Cell
Volume: 24
Issue: 17
Pages: 2633-44
DOI
Related Report
Peer Reviewed
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