| Project/Area Number |
25293044
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
Takei Shiro 浜松医科大学, 医学部, 特任助教 (60398576)
Nakashima Yuko 浜松医科大学, 医学部, 特任研究員 (20634760)
Kimura Yoshishige 浜松医科大学, 医学部, 助教 (90274703)
Shinriki Satoru 浜松医科大学, 医学部, 助教 (00583048)
Hayasaka Hayasaka 浜松医科大学, 医学部, 助教 (90415927)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
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| Keywords | 血液 / 質量顕微鏡 / 一細胞 / 分子イメージング / 代謝物 / 血液細胞 / 一細胞解析 / 脂質 |
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| Outline of Final Research Achievements |
Using matrix-assisted laser desorption/ionization imaging mass spectrometry and time-of-flight secondary ion mass spectrometry, we succeeded in single-cell imaging mass spectrometry of lipid by optimizing slide preparation for FACS-isolated blood cells and sample fixation. By single-cell imaging mass spectrometry we revealed different lipid composition in lymphocyte subsets and lipid metabolic change in breast cancer cell line, breast cancer stem cells and multiple myeloma (MM) cells. In addition we showed that palmitic acid, which was deceased in MM cells, is a potential multiple myeloma-specific drug.
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