Clarification of molecular and physiological properties of the acid-preventing barrier in gastric parietal cells
| Project/Area Number |
25293047
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | University of Toyama |
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Principal Investigator |
Sakai Hideki 富山大学, 大学院医学薬学研究部(薬学), 教授 (60242509)
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| Co-Investigator(Kenkyū-buntansha) |
SAWAGUCHI Akira 宮崎大学, 医学部, 教授 (30336292)
IKARI Akira 岐阜薬科大学, 薬学部, 教授 (50315850)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2013: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Keywords | 生理学 / 細胞・組織 / 胃酸分泌 / 細胞防御 / イオン輸送 / プロトンポンプ / イオンチャネル |
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| Outline of Final Research Achievements |
Though the apical membrane of the gastric parietal cell is exposed to the severe pH fluid (pH 0.8), the cell is not usually damaged by acid, but its mechanism has not been elucidated. We previously found that the unidentified Cl- channel in the basolateral membrane of gastric parietal cells participates in the cytoprotective mechanism. In this study, we clarified the following points: 1) Gastric tubulovesicles have variety in their characteristics, and the expression pattern of ion transporting proteins is different among the vesicles. 2) A Cl- channel function of SLC26A7 in the basolateral membrane is essential to the cytoprotective mechanism of gastric parietal cells. 3) The sialic acid at the end of the carbohydrate chain in gastric proton pump beta-subunit may be important for regulation of the pump activity and the cytoprotective mechanism.
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Report
(4 results)
Research Products
(61 results)
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[Journal Article] Clathrin-dependent endocytosis of claudin-2 by DFYSP peptide causes lysosomal damage in lung adenocarcinoma A549 cells.2015
Author(s)
Ikari A, Taga S, Watanabe R, Sato T, Shimobaba S, Sonoki H, Endo S, Matsunaga T, Sakai H, Yamaguchi M, Yamazaki Y, Sugatani J.
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Journal Title
Biochim Biophys Acta.
Volume: 1848
Issue: 10
Pages: 2326-2336
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Tight junctional localization of claudin-16 is regulated by syntaxin 8 in renal tubular epithelial cells2014
Author(s)
Akira Ikari, Chie Tonegawa, Ayumi Sanada, Toru Kimura, Hideki Sakai, Hisayoshi Hayashi, Hajime Hasegawa, Masahiko Yamaguchi, Yasuhiro Yamazaki, Satoshi Endo, Toshiyuki Matsunaga, Junko Sugatani
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Journal Title
J. Biol. Chem.
Volume: 289
Issue: 19
Pages: 13112-13123
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Functional coupling of chloride-proton exchanger C1C-5 to gastric H +, K <+->ATPase.2014
Author(s)
Takahashi Y, Fujii T, Fujita K, Shimizu T, Higuchi T, Tabuchi Y, Sakamoto H, Naito I, Manabe K, Uchida S, Sasaki S, Ikari A, Tsukada K, Sakai H.
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Journal Title
Biol. Open
Volume: 3巻
Issue: 1
Pages: 12-21
DOI
Related Report
Peer Reviewed
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[Presentation] 胃細胞の分泌膜界面の構成と機能2015
Author(s)
酒井秀紀，藤井拓人，清水貴浩
Organizer
生理研研究会「第3回生体界面研究会」
Place of Presentation
自然科学研究機構 生理学研究所（愛知県岡崎市）
Year and Date
2015-07-16
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