| Project/Area Number |
25293055
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental physiology(including physical medicine and nutritional physiology)
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
UETA Yoichi 産業医科大学, 医学部, 教授 (10232745)
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| Co-Investigator(Kenkyū-buntansha) |
HASHIMOTO Hirofumi 産業医科大学, 医学部, 准教授 (10454935)
MARUYAMA Takashi 産業医科大学, 医学部, 助教 (20533194)
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| Co-Investigator(Renkei-kenkyūsha) |
MIZUMURA Kazue 中部大学, 生命健康科学部, 教授 (00109349)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Keywords | 浸透圧 / メカノセンサー / バゾプレッシン / 蛍光タンパク / 遺伝子改変動物 / 飲水 |
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| Outline of Final Research Achievements |
The purpose of the study was to investigate novel osmo-/mechano-sensing molecules and osmosensitive mechanisms in the central nervous system, including the hypothalamo-neurohypophysial system. We visualized the central osmosensitive areas after acute osmotic challenge, using c-fos-eGFP transgenic rats that express c-fos and eGFP fusion gene after adequate physiological stimuli. We found that osomotic and non-osmotic stimuli such as mechanical and hypovolemic stimuli caused activation of vasopressin-eGFP magnocellular neurosecretory cells in vasopressin-eGFP transgenic rats. We also found that TRPV1 and TRPV4 knockout mice showed different drinking behaviors in comparison with wild type.
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