Analyses of the electrochemical properties in the unique extracellular solution of the inner ear and their possible involvement in pathological processes of deafness
| Project/Area Number |
25293058
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
General pharmacology
|
|---|
| Research Institution | Niigata University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NAKAJIMA Masato 新潟大学, 医歯学総合病院, 特任助教 (60588250)
NIN Fumiaki 新潟大学, 医歯学系, 准教授 (80644905)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
YAMAUCHI Takeshi 新潟大学, 自然科学系, 教授 (90262477)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
|
|---|
| Keywords | 内耳 / イオン / 水 / 電気生理 / 数理モデル |
|---|
| Outline of Final Research Achievements |
Deafness provides a strong impact for human society. To overcome this disease in future, in this study we focused the cochlea of the inner ear. This organ is filled with an unusual extracellular fluid that exhibits a highly positive potential and a high [K+]. We examined the mechanism underlying formation of these unique properties and their possible relevance to water movement. The endolymph is maintained by an epithelial tissue, stria vascularis. By using in vivo electrophysiological approaches, biochemical techniques and computational modeling, we showed functionality of several ion transport molecules in the stria and their roles in the maintenance of the endolymphatic properties. Moreover, we performed comprehensive analysis of membrane proteins of the stria and identified numerous ion transport molecules whose expression had not been reported in the tissue. These results may contribute to elucidation of pathological processes of deafness and development of the medical therapies.
|
Report
(4 results)
Research Products
(75 results)
-
[Journal Article] Heterozygous mutation of Ush1g/Sans in mice causes early-onset progressive hearing loss, which is recovered by reconstituting the strain-specific mutation in Cdh232016
Author(s)
Miyasaka Y, Shitara H, Suzuki S, Yoshimoto S, Seki Y, Ohshiba Y, Okumura K, Taya C, Tokano H, Kitamura K, Takada T, Hibino H, Shiroishi T, Kominami R, Yonekawa H, Kikkawa Y
-
Journal Title
Hum. Mol. Genet.
Volume: 印刷中
Issue: 10
Pages: 2045-2059
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
-
-
-
[Journal Article] Molecular architecture of the stria vascularis membrane transport system, which is essential for physiological functions of the mammalian cochlea.2015
Author(s)
Uetsuka S, Ogata G, Nagamori S, Isozumi N, Nin F, Yoshida T, Komune S, Kitahara T, Kikkawa Y, Inohara H, Kanai Y, Hibino H.
-
Journal Title
Eur J Neurosci.
Volume: 42
Issue: 3
Pages: 1984-2002
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Transient induction of deafness by optogenesis targeting the endocochlear potential in the inner ear.2016
Author(s)
Mitsuo P. Sato, Fumiaki Nin, Taiga Higuchi, Satoru Uetsuka, Takamasa Yoshida, Masatsugu Masuda, Takahisa Watabe, Sho Kanzaki,Kaoru Ogawa, Katsumi Doi, Hirohide Takebayashi, Kenji Tanaka, Hiroshi Hibino
Organizer
Association for Research in Otolaryngology 39th MidWinter Meeting
Place of Presentation
San Diego（USA）
Year and Date
2016-02-20
Related Report
Int'l Joint Research
-
-
-
-
-
-
[Presentation] Transient induction of deafness by optogenesis targeting the endocochlear potential in the inner ear.2015
Author(s)
Mitsuo P Sato, Fumiaki Nin, Taiga Higuchi, Satoru Uetsuka, Takamasa Yoshida, Sizuo Komune, Hidenori Inohara, Katsumi Doi, Hirohide Takebayashi, Kenji Tanaka, Hiroshi Hibino
Organizer
Inner ear biology 2015
Place of Presentation
Universita' Cattolica del Sacro Cuore（Rome）
Year and Date
2015-09-12
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
-
[Presentation] The mechanism underlying the potassium transport of the lateral wall in the cochlea.2015
Author(s)
Takamasa Yoshida, Fumiaki Nin, Naoko Adachi, Genki Ogata, Satoru Uetsuka, Mitsuo Sato, Hidenori Inohara, Yasuo Hisa, Shizuo Komune, Yoshihisa Kurachi, Hiroshi Hibino
Organizer
30th Politzer Society Meeting
Place of Presentation
朱鷺メッセ（新潟市中央区）
Year and Date
2015-07-01
Related Report
Int'l Joint Research
-
[Presentation] Proteomic analysis of membrane transport systems of the epithelial tissue in the mammalian cochlea.2015
Author(s)
Satoru Uetsuka, Genki Ogata, Shushi Nagamori, Noriyoshi Isozumi, Fumiaki Nin, Takamasa Yoshida, Tadashi Kitahara, Yoshiaki Kikkawa, Hidenori Inohara, Yoshikatsu Kanai, Hiroshi Hibino
Organizer
30th Politzer Society Meeting
Place of Presentation
朱鷺メッセ（新潟市中央区）
Year and Date
2015-07-01
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] 聴覚の不思議2014
Author(s)
日比野 浩
Organizer
大阪人工内耳フォーラム2014
Place of Presentation
大阪
Year and Date
2014-08-23
Related Report
-
-
-
-
-
-
-
-
-
-
-
