| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2014: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
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| Outline of Final Research Achievements |
Protein arginine methylation is a post-translational modification which contributes to a wide range of biological processes such as transcriptional control and mRNA splicing. Mice lacking PRMT5 specifically in osteoclasts showed an decreased bone mass due to an increased bone resorption. In addition, T cell-specific deletion of the Prmt5 gene led to a marked reduction in gamma chain family cytokine signaling and a severe loss of thymic iNKT cells and a decreased number of peripheral CD4+ and CD8+ T cells. PRMT5 induces the symmetric dimethylation of Sm proteins which promote the splicing of the Il2rg pre-mRNA, critically contributing to the expression of gamma chain. These findings demonstrate that arginine methylation plays a key role in the regulation of gamma chain family cytokine signaling strength by facilitating the expression of signal-transducing components.
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