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Integrated understanding of quality control, membrane insertion, and membrane fusion of SNARE proteins

Research Project

Project/Area Number 25293071
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field General medical chemistry
Research InstitutionKobe University

Principal Investigator

SAKISAKA TOSHIAKI  神戸大学, 医学研究科, 教授 (80359843)

Co-Investigator(Kenkyū-buntansha) 山本 泰憲  神戸大学, 医学研究科, 准教授 (30467659)
Project Period (FY) 2013-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2017: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywords小胞輸送 / SNAREタンパク質 / 品質管理 / 膜挿入 / 膜融合 / 神経科学
Outline of Final Research Achievements

Vesicle traffic plays important roles in various cellular events, such as exocytosis of hormone or digestive enzyme, and endocytosis of growth factor. SNARE proteins are essential for vesicle traffic. The amount of v-SNARE proteins on the vesicle and the amount of t-SNARE proteins on the target membrane regulate the speed of vesicle fusion. We have identified membrane insertion complex for SNARE proteins, CAML complex. To elucidate regulatory mechanism of membrane insertion of SNARE proteins, we identified p47 as a CAML-interacting protein. We also revealed subcellular localization mechanism of a SNARE protein lacking SNARE motif, Sec22c.

Report

(6 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (23 results)

All 2018 2017 2016 2015 2014 2013 Other

All Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Open Access: 5 results,  Acknowledgement Compliant: 4 results) Presentation (8 results) Book (2 results) Remarks (4 results)

  • [Journal Article] The peroxisome biogenesis factors posttranslationally target reticulon homology domain-containing proteins to the endoplasmic reticulum membrane.2018

    • Author(s)
      Yamamoto, Y. and Sakisaka, T.
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 2322-2322

    • DOI

      10.1038/s41598-018-20797-0

    • NAID

      120006728099

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The number of the C-terminal transmembrane domains has the potency to specify subcellular localization of Sec22c.2017

    • Author(s)
      Yamamoto, Y., Yurugi, C., and Sakisaka, T.
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 487 Issue: 2 Pages: 388-395

    • DOI

      10.1016/j.bbrc.2017.04.071

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Kif14 overexpression accelerates murine retinoblastoma development.2016

    • Author(s)
      O'Hare, M., Shadmand, M., Sulaiman, R.S., Sishtla, K., Sakisaka, T., and Corson, T.W.
    • Journal Title

      Int. J. Cancer.

      Volume: 139巻8号 Issue: 8 Pages: 1752-1758

    • DOI

      10.1002/ijc.30221

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The Emerging Role of Calcium-modulating Cyclophilin Ligand (CAML) in Posttranslational Insertion of Tail-anchored Proteins into the Endoplasmic Reticulum Membrane.2015

    • Author(s)
      Yamamoto, Y., and Sakisaka, T.
    • Journal Title

      The Journal of Biochemistry

      Volume: 印刷中 Issue: 6 Pages: 419-429

    • DOI

      10.1093/jb/mvv035

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Crystal structure of afadin PDZ domain-nectin-3 complex shows the structural plasticity of the ligand-binding site.2015

    • Author(s)
      Fujiwara Y, Goda N, Tamashiro T, Narita H, Satomura K, Tenno T, Nakagawa A, Oda M, Suzuki M, Sakisaka T, Takai Y, Hiroaki H.
    • Journal Title

      Protein Sci.

      Volume: 24 Issue: 3 Pages: 376-385

    • DOI

      10.1002/pro.2628

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cytoarchitecture of the olfactory bulb in the laggard mutant mouse.2014

    • Author(s)
      Yunus J, Setsu T, Kikkawa S, Sakisaka T, Terashima T.
    • Journal Title

      Neuroscience

      Volume: 275 Pages: 259-271

    • DOI

      10.1016/j.neuroscience.2014.06.011

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification and Characterization of TMEM33 as a Reticulon-binding Protein.2014

    • Author(s)
      Urade, T., Yamamoto, Y., Zhang, X., Ku, Y., and Sakisaka, T.
    • Journal Title

      Kobe Journal of Medical Sciences

      Volume: 60

    • NAID

      110009842833

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] VAP-B Binds to Rab3GAP1 at the ER: Its Implication in Nuclear Envelope Formation through the ER-Golgi Intermediate Compartment.2014

    • Author(s)
      Hantan, D., Yamamoto, Y., and Sakisaka, T.
    • Journal Title

      Kobe Journal of Medical Sciences

      Volume: 60

    • NAID

      110009831834

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Arl6IP1 has the ability to shape the mammalian ER membrane in a reticulon-like fashion.2014

    • Author(s)
      Yamamoto, Y., Yoshida, A., Miyazaki, N., Iwasaki, K., and Sakisaka, T.
    • Journal Title

      Biochemical Journal

      Volume: 458 Issue: 1 Pages: 69-79

    • DOI

      10.1042/bj20131186

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] ペルオキシソーム形成因子による小胞体膜変形タンパ ク質reticulonの翻訳後膜挿入機構2017

    • Author(s)
      山本泰憲,匂坂敏朗
    • Organizer
      2017年度生命科学系学会合同年次大会(ConBio2017)
    • Related Report
      2017 Annual Research Report
  • [Presentation] ユビキチンリガーゼ活性による小胞体の新しい形態調 節機構2017

    • Author(s)
      梶保博昭,山本泰憲, 匂坂敏朗
    • Organizer
      2017年度生命科学系学会合同年次大会(ConBio2017)
    • Related Report
      2017 Annual Research Report
  • [Presentation] 小胞輸送調節タンパク質Sec22CはC末膜貫通領域の数により細胞内局在を制御する2017

    • Author(s)
      山本泰憲,萬木千聖,匂坂敏朗
    • Organizer
      第64回 日本生化学会 近畿支部例会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 小胞体膜タンパク質による極長鎖脂肪酸合成の制御2017

    • Author(s)
      内田安則,山本泰憲, 出来宏晃, 匂坂敏朗
    • Organizer
      第64回 日本生化学会 近畿支部例会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ユビキチンリガーゼによる小胞体の形態制御機構2017

    • Author(s)
      梶保博昭,姜 山、山本泰憲, 匂坂敏朗
    • Organizer
      第64回 日本生化学会 近畿支部例会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 髄鞘低形成と皮質形成異常を示す新規突然変異マウスlaggard2016

    • Author(s)
      匂坂 敏朗、寺島 俊雄
    • Organizer
      第121回日本解剖学会総会・全国学術集会
    • Place of Presentation
      ビッグパレット福島
    • Year and Date
      2016-03-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] 小胞体の膜形態制御と翻訳後膜挿入の分子機構2014

    • Author(s)
      山本 泰憲、匂坂 敏朗
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2014-10-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] Reticulon結合タンパク質TMEM33の同定と性状解析2013

    • Author(s)
      張 霞, 山本 泰憲, 浦出 剛史, 匂坂 敏朗
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Annual Research Report
  • [Book] DOJIN BIOSCIENCEシリーズ『メンブレントラフィック』 PartIII: 高次生体機能を支えるメンブレントラフィック 10章 神経伝達物質放出を支えるメンブレントラフィック2016

    • Author(s)
      山本 泰憲、匂坂 敏朗
    • Publisher
      化学同人
    • Related Report
      2015 Annual Research Report
  • [Book] Presynaptic Terminals2015

    • Author(s)
      Yamamoto, Y., and Sakisaka, T.
    • Total Pages
      365
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Remarks] 膜動態学研究室ホームページ

    • URL

      http://www.med.kobe-u.ac.jp/membrd/

    • Related Report
      2017 Annual Research Report
  • [Remarks]

    • URL

      http://www.med.kobe-u.ac.jp/membrd/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 神戸大学大学院医学研究科 生理学・細胞生物学講座 膜動態学分野

    • URL

      http://www.med.kobe-u.ac.jp/membrd/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 神戸大学大学院医学研究科 生理学・細胞生物学講座 膜動態学分野

    • URL

      http://www.med.kobe-u.ac.jp/membrd/

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

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