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Signal transduction during erythrocyte invasion by malaria parasites

Research Project

Project/Area Number 25293102
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Parasitology (including sanitary zoology)
Research InstitutionNagasaki University

Principal Investigator

KANEKO Osamu  長崎大学, 熱帯医学研究所, 教授 (50325370)

Co-Investigator(Renkei-kenkyūsha) YAHATA Kazuhide  長崎大学, 熱帯医学研究所, 助教 (40467965)
Research Collaborator ASADA Masahito  長崎大学, 熱帯医学研究所, 助教
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Keywords原虫 / マラリア / 侵入 / シグナル伝達 / 赤血球
Outline of Final Research Achievements

Malaria parasites invade into erythrocytes using molecules sequentially released from their apical microorganelles. However, the details of the signal transduction during erythrocyte invasion by the parasites is largely unknown. In this study, we tried to adapt the conditional knock-down/knock-out systems to the rodent malaria parasite Plasmodium yoelii aiming to apply the developed systems to answer above question. We were able to inducibly delete the gene loci for an exogenous mCherry or an non-essential protein in the transgenic P. yoelii, however, the gene locus of the essential molecule was not deleted by the induction. Thus we continued to optimize this systems in P. yoelii. In addition to this effort, we established a platform to examine parasite phenotypes during erythrocyte invasion by developing a method to isolate erythrocyte-invasive P. yoelii merozoites and a robust method to monitor the intracellular Ca2+ level of the malaria parasites.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (28 results)

All 2016 2015 2014 2013 Other

All Int'l Joint Research (4 results) Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (20 results) (of which Int'l Joint Research: 6 results,  Invited: 3 results)

  • [Int'l Joint Research] Nepal Academy of Science and Technology(ネパール)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] University of Minho(ポルトガル)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Nanyang Technological University(シンガポール)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] University of Geneva(スイス)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Ca2+ monitoring in Plasmodium falciparumusing the yellow cameleon-Nano biosensor.2016

    • Author(s)
      Pandey K, Ferreira PE, Ishikawa T, Nagai T, Kaneko O, Yahata K
    • Journal Title

      Sci Rep

      Volume: 6 Pages: 23454-23454

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Isolation of invasive Plasmodium yoelii merozoites with a long half-life to evaluate invasion dynamics and potential invasion inhibitors.2015

    • Author(s)
      Mutungi JK, Yahata K, Sakaguchi M, Kaneko O.
    • Journal Title

      Molecular and Biochemical Parasitology

      Volume: 204 Issue: 1 Pages: 26-33

    • DOI

      10.1016/j.molbiopara.2015.12.003

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The cytoplasmic region of Plasmodium falciparum SURFIN4.2 is required for transport from Maurer's clefts to the red blood cell surface2015

    • Author(s)
      Kagaya W, Miyazaki S, Yahata K, Ohta N, Kaneko O
    • Journal Title

      Tropical Medicine and Health

      Volume: 43(4) Issue: 4 Pages: 265-272

    • DOI

      10.2149/tmh.2015-38

    • NAID

      130005130397

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Expression and localization of rhoptry neck protein 5 in merozoites and sporozoites of Plasmodium yoelii.2014

    • Author(s)
      Mutungi JK, Yahata K, Sakaguchi M, *Kaneko O.
    • Journal Title

      Parasitol Int

      Volume: 63 Issue: 6 Pages: 794-801

    • DOI

      10.1016/j.parint.2014.07.013

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] ネズミマラリア原虫におけるTet-Onによる遺伝子コンディショナルノックアウト法の開発2016

    • Author(s)
      麻田正仁、外川裕人、矢幡一英、金子修
    • Organizer
      第85回日本寄生虫学会大会
    • Place of Presentation
      宮崎市民プラザ(宮崎県宮崎市)
    • Year and Date
      2016-03-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] Plasmodium yoelii遺伝子を標的としたテトラサイクリン発現誘導システムの確立2016

    • Author(s)
      外川裕人、麻田正仁、矢幡一英、金子修
    • Organizer
      第85回日本寄生虫学会大会
    • Place of Presentation
      宮崎市民プラザ(宮崎県宮崎市)
    • Year and Date
      2016-03-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] 熱帯熱マラリア原虫SURFIN4.2の細胞内領域はマウレル裂から赤血球表面への輸送に必要である2016

    • Author(s)
      加賀谷渉、宮崎真也、矢幡一英、太田伸生、金子修
    • Organizer
      第85回日本寄生虫学会大会
    • Place of Presentation
      宮崎市民プラザ(宮崎県宮崎市)
    • Year and Date
      2016-03-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] ネズミマラリア原虫赤血球侵入リガンドEBL領域4 の機能解析2016

    • Author(s)
      大槻均,菅井千明,近藤陽子,入子英幸,石野智子,金子修,坪井敬文,鳥居本美
    • Organizer
      第85回日本寄生虫学会大会
    • Place of Presentation
      宮崎市民プラザ(宮崎県宮崎市)
    • Year and Date
      2016-03-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] Isolation method of invasive Plasmodium yoelii merozoites with a long half-life to evaluate erythrocyte invasion dynamics and potential invasion inhibitors.2016

    • Author(s)
      Mutungi JK, Yahata K, Sakaguchi M, Kaneko O
    • Organizer
      Molecular Approach to Malaria 2016
    • Place of Presentation
      Lorne (Austraria)
    • Year and Date
      2016-02-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor.2016

    • Author(s)
      Yahata K, Pandey K, Ferreira PE, Ishikawa T, Kaneko O
    • Organizer
      Molecular Approach to Malaria 2016
    • Place of Presentation
      Lorne (Austraria)
    • Year and Date
      2016-02-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor2016

    • Author(s)
      Pandey K, Ferreira PE, Ishikawa T, Kaneko O, Yahata K
    • Organizer
      U.S.-Japan Cooperative Medical Sciences Program (USJCMSP)
    • Place of Presentation
      Bethesda (USA)
    • Year and Date
      2016-01-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 熱帯熱マラリア原虫SURFIN4.2のトリプトファン豊富領域はマウレル裂から赤血球表面への輸送に必要である2015

    • Author(s)
      加賀谷渉、宮崎真也、矢幡一英、太田伸生、金子修
    • Organizer
      第56回日本熱帯医学会大会
    • Place of Presentation
      大阪大学コンベンションセンター(大阪府吹田市)
    • Year and Date
      2015-12-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] Isolation method of invasive Plasmodium yoelii merozoites with a long half-life to evaluate erythrocyte invasion dynamics and potential invasion inhibitors.2015

    • Author(s)
      Mutungi JK, Yahata K, Sakaguchi M, Kaneko O
    • Organizer
      2nd International Symposium & 4th Annual Researche Meeting on Matryoshka-Type Evolution of Eukaryotic Cells
    • Place of Presentation
      Tsukuba University (Tsukuba, Ibaraki)
    • Year and Date
      2015-09-30
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ca2+ monitoring in Plasmodium falciparum cytosol through yellow cameleon-Nano biosensors2015

    • Author(s)
      Yahata K, Pandey K, Ferreira PE, Ishikawa T, Kaneko O
    • Organizer
      Protein island Matsuyama International Symposium
    • Place of Presentation
      松山市男女共同参画推進センター(愛媛県松山市)
    • Year and Date
      2015-09-24
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Calcium monitoring in Plasmodium falciparum through Yellow Cameleon-Nano Biosensors2015

    • Author(s)
      Yahata K, Pandey K, Ferreira PE, Ishikawa T, Kaneko O
    • Organizer
      The 14th Awaji International Forum on Infection and Immunity
    • Place of Presentation
      Awaji Yumebutai International Conference Center (Awaji, Hyogo)
    • Year and Date
      2015-09-08
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of Tet-On-based conditional gene knockout system for rodent malaria parasite Plasmodium yoelii2015

    • Author(s)
      Asada M, Kegawa Y, Yahata K, Kaneko O
    • Organizer
      第4回感染症若手フォーラム
    • Place of Presentation
      アテーナ海月(兵庫県淡路市)
    • Year and Date
      2015-09-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] Plasmodium yoelii 赤血球内侵入型原虫を標的としたテトラサイクリン発現誘導システムの確立2015

    • Author(s)
      外川裕人, 麻田正仁, 矢幡一英, 金子修
    • Organizer
      第23回分子寄生虫学ワークショップ/第13回分子寄生虫・マラリア研究フォーラム
    • Place of Presentation
      帯広畜産大学(北海道帯広市)
    • Year and Date
      2015-08-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] Evaluation of Ca2+ Oscillation in Plasmodium falciparum through Yellow Cameleon-Nano Biosensors2015

    • Author(s)
      Kaneko O
    • Organizer
      The 12th NUS-Nagasaki Joint Symposium
    • Place of Presentation
      Singapore (Singapore)
    • Year and Date
      2015-06-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ネズミマラリア原虫赤血球侵入リガンドEBL 領域3-5の機能解析2015

    • Author(s)
      大槻均、菅井千明、入子英幸、石野 智子、金子修、福本宗嗣、坪井敬文、鳥居本美
    • Organizer
      第84回日本寄生虫学会大会
    • Place of Presentation
      杏林大学三鷹キャンパス、三鷹市
    • Year and Date
      2015-03-21 – 2015-03-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] Isolation of viable and invasive Plasmodium yoelii merozoite2014

    • Author(s)
      Mutungi JK, Yahata K, Sakaguchi M, Kaneko O.
    • Organizer
      The 13rd Awaji International Forum on Infection and Immunity.
    • Place of Presentation
      Nara Prefectural New Public Hall, Nara, Japan
    • Year and Date
      2014-09-23 – 2014-09-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] Characterization of PyRON5, a Plasmodium yoelii rhoptry neck protein.2014

    • Author(s)
      Mutungi JK, Yahata K, Sakaguchi M, Kaneko O.
    • Organizer
      25th Annual Molecular Parasitology Meeting
    • Place of Presentation
      Woods Hole, USA
    • Year and Date
      2014-09-14 – 2014-09-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] ネズミマラリア原虫P. yoelii でのテトラサイクリン発現誘導システムの確立2014

    • Author(s)
      外川裕人, 麻田正仁, 矢幡一英, 金子修
    • Organizer
      第22回分子寄生虫学ワークショップ/第12回分子寄生虫・マラリア研究フォーラム合同大会
    • Place of Presentation
      帯広畜産大学、帯広市
    • Year and Date
      2014-08-31 – 2014-09-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] ネズミマラリア原虫 Plasmodium yoelii のAMA1 はメロゾイトの形態変化にともない放出される2014

    • Author(s)
      矢幡一英, Mutungi JK, 坪井敬文, 金子修
    • Organizer
      第22回分子寄生虫学ワークショップ/第12回分子寄生虫・マラリア研究フォーラム合同大会
    • Place of Presentation
      帯広畜産大学、帯広市
    • Year and Date
      2014-08-31 – 2014-09-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] Plasmodium yoelii赤血球侵入機構の解析に向けたテトラサイクリン発現調節システムの開発2013

    • Author(s)
      麻田正仁,Xangsayarath P, 矢幡一英,金子 修
    • Organizer
      第21回分子寄生虫学ワークショップ
    • Place of Presentation
      神戸市立神戸セミナーハウス、神戸市
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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