Development of diagnostic system with high specificity and sensitivity for exosome derived from gynecologic cancer
| Project/Area Number |
25293133
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Toyo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
加藤 聖子 九州大学, 医学(系)研究科(研究院), 教授 (10253527)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2013: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Keywords | 腫瘍マーカー / 卵巣がん / EphA2 / 乳がん / エクソソーム / 抗体 / ELISA / 卵巣癌 / EpCAM / ナノファイバー / フローサイトメトリー / CD147 |
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| Outline of Final Research Achievements |
EphA2 is a cell surface glycoprotein expresses in various tumor tissues including lung, ovary and prostate carcinoma. EphA2 fragment is also released in a soluble form by the proteolytic cleavage from tumor cell surface. We tried to detect the soluble form of EphA2 (sEphA2) in the serum of various cancer patients. By using antibodies to EphA2, we developed a new sandwich ELISA system detecting human sEphA2 with high sensitivity and specificity. With this system, we measured the concentration of sEphA2 in the serum of normal healthy donors and various carcinoma patients. The concentration of sEphA2 was significantly higher in sera of lung, prostate, ovarian cancer patients than healthy control donors. The levels of sEphA2 in serum of lung cancer are correlated with CYFRA and CEA. In contrast, sEphA2 levels versus PSA or CA125 are not correlated. These data indicate that the diagnosis of lung, prostate and ovary cancer patients enabled by measuring the concentration of sEphA2.
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Report
(4 results)
Research Products
(19 results)
