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3D analysis of functional neural network of sensory input and output in the spinal cord by use of transgenic mice expressing fluorescent proteins

Research Project

Project/Area Number 25293137
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Pain science
Research InstitutionKansai Medical University

Principal Investigator

ITO Seiji  関西医科大学, 医学部, 教授 (80201325)

Co-Investigator(Kenkyū-buntansha) MATSUMURA Shinji  関西医科大学, 医学部, 講師 (70276393)
NISHIDA Kazuhiko  関西医科大学, 医学部, 助教 (80448026)
YAO Ikuko  関西医科大学, 医学部, 准教授 (60399681)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2013: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Keywords多光子励起顕微鏡 / 脊髄後角 / 神経回路網 / 子宮内遺伝子導入 / トランスジェニックマウス / 皮膚刺激 / Ca2+応答 / 形態変化 / 三叉神経脊髄路核 / in vivo / かゆみ刺激
Outline of Final Research Achievements

Peripheral sensory impulses produced by stimuli to the skin are transmitted to the spinal dorsal horn and recognized as touch and pain in the brain. In this study, we established a 3-dimensional system of neural network in the spinal cord by two-photon fluorescence microscope and functionally and morphologically analyzed the network in transgenic mice expressing fluorescent proteins.
We could observe dozens of neurons in the spinal dorsal horn in response to a stimulus to the skin and made stimulus-response maps. While neurons in the superficial layer responded to heat and mechanical stimuli, ones in the deeper layer responded to touch.
We also revealed that synapses were newly formed on neurites in the dorsal horn in inflammatory animal model and that the increase in synapses was blocked by glutamate antagonists. Thus we think that the research plans proposed by this grant have been achieved sufficiently.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report

Research Products

(40 results)

All 2016 2015 2014 2013 Other

All Journal Article Presentation Book Remarks

  • [Journal Article] Role of c-Jun N-terminal kinase in late nerve regeneration monitored by in vivo imaging of thy1-yellow fluorescent protein transgenic mice.2016

    • Author(s)
      Nguyen H. T., Katano, T., Matsumura, S., Pham M. V., Muratani, T., Minami, T. and Seiji Ito.
    • Journal Title

      European Journaol of Neuroscience

      Volume: 43 Pages: 548-560

    • DOI
      10.1111/ejn.13139
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] In vivo two-photon imaging of structural dynamics in the spinal dorsal horn in an inflammatory pain model2015

    • Author(s)
      Matsumura S, Taniguchi W, Nishida K, Nakatsuka T, Ito S
    • Journal Title

      Eur J Neurosci.

      Volume: 41 Pages: 987-995

    • DOI
      10.1111/ejn.12837
    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Comparison of mechanisms of allodynia induced by acromelic acid A between early and late phases.2015

    • Author(s)
      Omoto H, Matsumura S, Kitano M, Miyazaki S, Minami T, Ito S.
    • Journal Title

      Eur. J. Pharmacol.

      Volume: In Press Pages: 42-48

    • DOI
      10.1016/j.ejphar.2015.03.075
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Pituitary adenylate cyclase-activating polypeptide is regulated by alternative splicing of transcriptional repressor REST/NRSF in nerve injury.2015

    • Author(s)
      Shudo, Y., Shimojo, M., Fukunaga, M. and Ito, S.
    • Journal Title

      Life Sciences

      Volume: 143 Pages: 174-181

    • DOI
      10.1016/j.lfs.2015.10.033
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential regulation of RNA-ending enzymes, ADAR1 and ADAR2, expression by 5-aza-2’-deoxycytidine and trichostatin A in human neuronal SH-SY5Y cells.2015

    • Author(s)
      Uchida, H. and Ito, S.
    • Journal Title

      Neuroreport

      Volume: 26 Pages: 1089-1094

    • DOI
      10.1097/wnr.0000000000000474
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nocistatin: milestone of one decade of research.2015

    • Author(s)
      Okuda-Ashitaka, E., Ito, S.
    • Journal Title

      Current pharmaceutical design

      Volume: 21 Pages: 868-884

    • DOI
      10.2174/1381612820666141027112451
    • Related Report
      2015 Annual Research Report
  • [Journal Article] Involvement of endothelin B receptor in peripheral nerve regeneration using sciatic nerve transection-regeneration model2015

    • Author(s)
      Nguyen H. T., Katano, T., Matsumura, S. and Ito, S.
    • Journal Title

      PAIN RESEARCH

      Volume: 30 Issue: 3 Pages: 167-172

    • DOI
      10.11154/pain.30.167
    • NAID
      130005093937
    • Related Report
      2015 Annual Research Report
    • Open Access
  • [Journal Article] 脊髄に起因する痛み~脊髄の構造と慢性化をおこす中枢性感作~Chronic pain is induced by central sensitization in spinal dorsal horn.2015

    • Author(s)
      片野泰代、伊藤誠二
    • Journal Title

      Practice of Pain Management

      Volume: 6 Pages: 22-25

    • Related Report
      2014 Annual Research Report
  • [Journal Article] 神経障害性痛の臨床2015

    • Author(s)
      伊藤誠二
    • Journal Title

      痛みのScience & Practice

      Volume: 8

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Transcriptional mechanisms link epithelial plasticity to adhesion and differentiation of epidermal progenitor cells.2014

    • Author(s)
      Lee, B., Villarreal-Ponce, A., Fallahi, M., Ovadia, J., Sun, P., Yu, Q.C., Ito, S., Sinha, S., Nie, Q. and Dai, X.
    • Journal Title

      Developmental cell.

      Volume: 29 Pages: 47-58

    • DOI
      10.1016/j.devcel.2014.03.005
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Three-dimensional distribution of sensory stimulation-evoked neuronal activity of spinal dorsal horn neurons analyzed by in vivo calcium imaging.2014

    • Author(s)
      Nishida K, Matsumura S, Taniguchi W, Uta D, Furue H, Ito S.
    • Journal Title

      PLoS ONE

      Volume: 9(8)

    • DOI
      10.1371/journal.pone.0103321
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Involvement of Nax sodium channel in peripheral nerve regeneration via lactate signaling.2014

    • Author(s)
      Unezaki, S., Katano, T., Hiyama, T.Y., Nguyen, H. T., Yoshii, S., Noda, M., and Ito, S.
    • Journal Title

      Eur. J. Neurosci.

      Volume: 39 Pages: 720-729

    • DOI
      10.1111/ejn.12436
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification of nitrated tyrosine residues of protein kinase G-Iα by mass spectrometry.2014

    • Author(s)
      Lu J, Yao I, Shimojo M, Katano T, Uchida H, Setou M, Ito S
    • Journal Title

      Analytical and bioanalytical chemistry.

      Volume: 406 Pages: 1387-1396

    • DOI
      10.1007/s00216-013-7535-4
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 脊髄後角における新規慢性疼痛関連分子の同定2015

    • Author(s)
      片野泰代、福田正史、山崎真耶、阿部学、渡辺雅彦、古江秀昌、矢尾育子、西田和彦、奥村宣明、中澤敬信、山本雅、崎村健司、高尾敏文、伊藤誠二
    • Organizer
      2015年度包括型脳科学研究推進支援ネットワーク 冬のシンポジウム
    • Place of Presentation
      一橋大学 一橋講堂(東京都千代田区)
    • Year and Date
      2015-12-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] New mechanisms for pain control and clinical potential.2015

    • Author(s)
      Nguyen, H. T., Katano, T., Matsumura, S. and Ito, S.
    • Organizer
      International Conference on Pain-Anesthesiology and pain control-
    • Place of Presentation
      ハノイ市(ベトナム)
    • Year and Date
      2015-11-20
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Involvement of c-Jun N-terminal kinase in neurite extension of cultured DRG neurons.2015

    • Author(s)
      Nguyen, H. T., Matsumura, S. Katano, T., and Ito, S.
    • Organizer
      The 45th annual meeting of the Society for Neuroscience
    • Place of Presentation
      シカゴ(アメリカ)
    • Year and Date
      2015-10-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Localization of neuropathic pain-related protein, BEGAIN in the spinal dorsal horn.2015

    • Author(s)
      Katano, T., Watanabe, M., Yamazaki, M., Abe, M., Yao, I., Sakimura, K. and Ito, S.
    • Organizer
      The 45th annual meeting of the Society for Neuroscience
    • Place of Presentation
      シカゴ(アメリカ)
    • Year and Date
      2015-10-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Chasing morphological changes of neuronal processes in the spinal dorsal horn in an inflammatory pain model by using two-photon microscopy.2015

    • Author(s)
      Matsumura, S., Taniguchi, W., Nishida, K., Nakatsuka, T. and Ito S.
    • Organizer
      The 45th annual meeting of the Society for Neuroscience
    • Place of Presentation
      シカゴ(アメリカ)
    • Year and Date
      2015-10-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Selective siRNA-mediated suppression of nSR100(SRRM4) induces the cell death of small cell lung cancer.2015

    • Author(s)
      Shimojo, M., Shudo, Y., Ito, S.
    • Organizer
      The 45th annual meeting of the Society for Neuroscience
    • Place of Presentation
      シカゴ(アメリカ)
    • Year and Date
      2015-10-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Two feasible mouse models for neuropathy.2015

    • Author(s)
      Pham, M.V., Nguyen, H.T., Katano, T., Matsumura, S. and Ito, S.
    • Organizer
      The 2nd Gene & Immunotherapy Conference in Vietnam 2015
    • Place of Presentation
      ホーチミン市(ベトナム)
    • Year and Date
      2015-09-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 質量顕微鏡によるテストステロンのマウス精巣における可視化 Visualization of testosterone on mouse testis with imaging mass spectrometry.2015

    • Author(s)
      熊田アンリオバディア、谷口久哲、矢尾育子、古田享史、松田公志、伊藤誠二
    • Organizer
      第40回日本医用マススペクトル学会年会
    • Place of Presentation
      アクトシティ浜松コングレスセンター(静岡県浜松市)
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] Involvement of endothelin in peripheral nerve regeneration.2014

    • Author(s)
      Ito, S., Nguyen, H. T., Matsumura, S. and Katano, T.
    • Organizer
      The 44th annual meeting of the Society for Neuroscience
    • Place of Presentation
      Washington D.C. (U.S.A.)
    • Year and Date
      2014-11-15 – 2014-11-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Involvement of c-Jun N-terminal kinase in peripheral nerve regeneration.2014

    • Author(s)
      Nguyen, H. T., Katano, T., Matsumura, S. and Ito, S.
    • Organizer
      The 44th annual meeting of the Society for Neuroscience
    • Place of Presentation
      Washington D.C. (U.S.A.)
    • Year and Date
      2014-11-15 – 2014-11-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] CaMKIIキナーゼ不活型KIマウスにおける慢性疼痛モデル脊髄後角のCaMKIIのシナプス局在2014

    • Author(s)
      矢尾育子、松村伸治、片野泰代、山肩葉子、井本敬二、伊藤誠二
    • Organizer
      第37回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] In vivo カルシウムイメージングを用いた皮膚の異なる点への感覚刺激に応答する脊髄後角ニューロンの解析2014

    • Author(s)
      西田和彦、松村伸治、伊藤誠二
    • Organizer
      第37回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 成熟した疼痛研究の新しい展開 New departure of mature pain research.2014

    • Author(s)
      伊藤誠二、片野泰代、松村伸治、西田和彦
    • Organizer
      日本ペインクリニック学会第48回大会
    • Place of Presentation
      京王プラザホテル(東京都・新宿区)
    • Year and Date
      2014-07-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 末梢神経再生モデルを用いた神経再生におけるエンドセリンの関与 Involvement of endothelin in peripheral nerve regeneration using tubing and osmotic pump model.2014

    • Author(s)
      Nguyen Huu Tu、片野泰代、松村伸治、伊藤誠二
    • Organizer
      第36回日本疼痛学会
    • Place of Presentation
      KKRホテルオーサカ(大阪府・大阪市)
    • Year and Date
      2014-06-20 – 2014-06-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Three-dimensional distribution of sensory stimulation-evoked neuronal activity of spinal dorsal horn neurons analyzed by in vivo calcium imaging. In vivo カルシウムイメージングによる脊髄後角ニューロンの神経活動の三次元分布の解析2013

    • Author(s)
      西田和彦、松村伸治、伊藤誠二
    • Organizer
      第36回日本神経科学大会・第56回日本神経化学会大会・第23回日本神経回路学会大会合同大会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Epigenetic gene regulation of RNA editing enzyme in neuron. 神経細胞におけるRNA編集酵素のエピゲノム制御2013

    • Author(s)
      内田仁司、伊藤誠二
    • Organizer
      第15回日本RNA学会年会
    • Place of Presentation
      ひめぎんホール(松山市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 神経因性疼痛におけるPituitary adenylate-cyclase activating polypeptide (PACAP)発現に関与する神経特異的転写抑制因子RE1-silencing transcription factor(REST/NRSF)アイソフォームに関する研究2013

    • Author(s)
      首藤由江、下條正仁、福永幹彦、伊藤誠二
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      国際展示場(神戸市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Involvement of neuropathic pain-related protein-B(NPRP-B), a novel functional molecule, in inflammatory and neuropathic pain in vivo.2013

    • Author(s)
      Katano, T., Yao, I., Yamazaki, M., Abe, M., Fukuda, M., Okumura, N., Takao, T., Sakimura, K. and Ito, S.
    • Organizer
      The 43rd annual meeting of the Society for Neuroscience
    • Place of Presentation
      San Diego, U.S.A.
    • Related Report
      2013 Annual Research Report
  • [Presentation] Proteomic approach of nitrated tyrosine residues of protein kinase G-Ia2013

    • Author(s)
      Ito, S., Lu, J., Shimojo, M., Katano, T., Uchida, H. and Yao, I.
    • Organizer
      The 43rd annual meeting of the Society for Neuroscience
    • Place of Presentation
      San Diego, U.S.A.
    • Related Report
      2013 Annual Research Report
  • [Presentation] Bifurcate roles of nitric oxide (NO) in neuropathic pain.2013

    • Author(s)
      Ito, S.
    • Organizer
      The 44th NIPS International Symposium and The 5th Asian Pain Symposium
    • Place of Presentation
      岡崎コンファレンスセンター(岡崎市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Book] 「分子脳科学」DOJIN BIOSCIENCE SERIES, 8章:体性感覚の受容と伝達の分子機構2015

    • Author(s)
      西田和彦、伊藤誠二
    • Total Pages
      312
    • Publisher
      化学同人
    • Related Report
      2015 Annual Research Report
  • [Book] PACAPと神経障害性痛 「痛みのScience & Practice 第8巻 臨床に役立つ神経障害性痛の理解」2015

    • Author(s)
      伊藤誠二
    • Total Pages
      285
    • Publisher
      文光堂
    • Related Report
      2015 Annual Research Report
  • [Book] Studies on Pediatric Disorders (Oxidative Stress in Applied Basic Research and Clinical Practice)2014

    • Author(s)
      Katano, T. Ito, S.
    • Total Pages
      494
    • Publisher
      Human Press
    • Related Report
      2013 Annual Research Report
  • [Book] Nociceptin Opioid2014

    • Author(s)
      Okuda-Ashitaka, E., Ito, S.
    • Publisher
      Academic Press
    • Related Report
      2013 Annual Research Report
  • [Book] DOJIN BIOSCIENCE SERIES 分子脳科学2014

    • Author(s)
      西田和彦、伊藤誠二
    • Publisher
      化学同人
    • Related Report
      2013 Annual Research Report
  • [Remarks] 関西医科大学医化学講座ホームページ

    • URL
      http://www3.kmu.ac.jp/medchem/index.html
    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
  • [Remarks] 関西医科大学 医化学講座ホームページ

    • URL
      http://www3.kmu.ac.jp/medchem/index.html
    • Related Report
      2013 Annual Research Report

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

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