3D analysis of functional neural network of sensory input and output in the spinal cord by use of transgenic mice expressing fluorescent proteins
| Project/Area Number |
25293137
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pain science
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| Research Institution | Kansai Medical University |
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Principal Investigator |
ITO Seiji 関西医科大学, 医学部, 教授 (80201325)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMURA Shinji 関西医科大学, 医学部, 講師 (70276393)
NISHIDA Kazuhiko 関西医科大学, 医学部, 助教 (80448026)
YAO Ikuko 関西医科大学, 医学部, 准教授 (60399681)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2013: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Keywords | 多光子励起顕微鏡 / 脊髄後角 / 神経回路網 / 子宮内遺伝子導入 / トランスジェニックマウス / 皮膚刺激 / Ca2+応答 / 形態変化 / 三叉神経脊髄路核 / in vivo / かゆみ刺激 |
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| Outline of Final Research Achievements |
Peripheral sensory impulses produced by stimuli to the skin are transmitted to the spinal dorsal horn and recognized as touch and pain in the brain. In this study, we established a 3-dimensional system of neural network in the spinal cord by two-photon fluorescence microscope and functionally and morphologically analyzed the network in transgenic mice expressing fluorescent proteins.
We could observe dozens of neurons in the spinal dorsal horn in response to a stimulus to the skin and made stimulus-response maps. While neurons in the superficial layer responded to heat and mechanical stimuli, ones in the deeper layer responded to touch.
We also revealed that synapses were newly formed on neurites in the dorsal horn in inflammatory animal model and that the increase in synapses was blocked by glutamate antagonists. Thus we think that the research plans proposed by this grant have been achieved sufficiently.
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Report
(4 results)
Research Products
(40 results)
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[Journal Article] Transcriptional mechanisms link epithelial plasticity to adhesion and differentiation of epidermal progenitor cells.2014
Author(s)
Lee, B., Villarreal-Ponce, A., Fallahi, M., Ovadia, J., Sun, P., Yu, Q.C., Ito, S., Sinha, S., Nie, Q. and Dai, X.
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Journal Title
Developmental cell.
Volume: 29
Pages: 47-58
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Involvement of Nax sodium channel in peripheral nerve regeneration via lactate signaling.2014
Author(s)
Unezaki, S., Katano, T., Hiyama, T.Y., Nguyen, H. T., Yoshii, S., Noda, M., and Ito, S.
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Journal Title
Eur. J. Neurosci.
Volume: 39
Pages: 720-729
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Localization of neuropathic pain-related protein, BEGAIN in the spinal dorsal horn.2015
Author(s)
Katano, T., Watanabe, M., Yamazaki, M., Abe, M., Yao, I., Sakimura, K. and Ito, S.
Organizer
The 45th annual meeting of the Society for Neuroscience
Place of Presentation
シカゴ（アメリカ）
Year and Date
2015-10-17
Related Report
Int'l Joint Research
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[Presentation] Involvement of neuropathic pain-related protein-B(NPRP-B), a novel functional molecule, in inflammatory and neuropathic pain in vivo.2013
Author(s)
Katano, T., Yao, I., Yamazaki, M., Abe, M., Fukuda, M., Okumura, N., Takao, T., Sakimura, K. and Ito, S.
Organizer
The 43rd annual meeting of the Society for Neuroscience
Place of Presentation
San Diego, U.S.A.
Related Report
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[Presentation] Proteomic approach of nitrated tyrosine residues of protein kinase G-Ia2013
Author(s)
Ito, S., Lu, J., Shimojo, M., Katano, T., Uchida, H. and Yao, I.
Organizer
The 43rd annual meeting of the Society for Neuroscience
Place of Presentation
San Diego, U.S.A.
Related Report
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