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Analysis of the origin of newly formed cardiomyocytes and their growth and mitotic factor by cardiac progenitor cell scaffold transplantation model.

Research Project

Project/Area Number 25293180
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Cardiovascular medicine
Research InstitutionChiba University

Principal Investigator

Nagai Toshio  千葉大学, 医学(系)研究科(研究院), 准教授 (00334194)

Co-Investigator(Kenkyū-buntansha) 小室 一成  東京大学, 医学部附属病院, 教授 (30260483)
Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Keywords心筋前駆細胞 / DNA傷害 / 細胞移植床 / 心筋再生 / パラクライン効果 / side population細胞 / 細胞移植
Outline of Final Research Achievements

Activation and subsequent induction into cardiomyocytes differentiation of endogenous cardiac stem or progenitor cells is a new strategy for cardiac regeneration. We examined the mechanisms of endogenous cardiac regeneration after epicardial engraftment of cardiac progenitor cell (CPC) scaffold. Transplantation of CPC scaffold reduced the DNA damage of cardiac side population cells. CPC scaffold secreted the several factors that promote angiogenesis, inhibit apoptotic cell death, or reduce emigration of neutrophils. Transplantation of CPC scaffold preserved the expression of aldehyde dehydrogenase 2 in the injured heart. We established a multi colored mouse heart model for lineage tracing. By using this model we quantified the dynamics of cardiomyocyte division after myocardial injury.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (12 results)

All 2016 2015 2014 2013

All Journal Article (7 results) (of which Peer Reviewed: 6 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results)

  • [Journal Article] Leukemia Inhibitory Factor Enhances Endogenous Cardiomyocyte Regeneration after Myocardial Infarction.2016

    • Author(s)
      Kanda M, Nagai T, Takahashi T, Liu ML, Kondou N, Naito AT, Akazawa H, Sashida G, Iwama A, Komuro I, Kobayashi Y.
    • Journal Title

      Plos One

      Volume: 11 Issue: 5 Pages: e0156562-e0156562

    • DOI

      10.1371/journal.pone.0156562

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Regeneration of the Cardiac Conduction System by Adipose Tissue-Derived Stem Cells2015

    • Author(s)
      Takahashi T, Nagai T, Kanda M, Liu ML, Kondo N, Naito AT, Ogura T, Nakaya H, Lee JK, Komuro I, Kobayashi Y.
    • Journal Title

      Circulation Journal

      Volume: 79 Issue: 12 Pages: 2703-2712

    • DOI

      10.1253/circj.CJ-15-0400

    • NAID

      130005110933

    • ISSN
      1346-9843, 1347-4820
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Complement C1q-induced activation of β-catenin signalling causes hypertensive arterial remodelling.2015

    • Author(s)
      Sumida T, Naito AT, Nomura S, Nakagawa A, Higo T, Hashimoto A, Okada K, Sakai T, Ito M, Yamaguchi T, Oka T, Akazawa H, Lee JK, Minamino T, Offermanns S, Noda T, Botto M, Kobayashi Y, Morita H, Manabe I, Nagai T, Shiojima I, Komuro I.
    • Journal Title

      Nat Commun

      Volume: 6 Issue: 1 Pages: 6241-6241

    • DOI

      10.1038/ncomms7241

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mitochondrial aldehyde dehydrogenase 2 plays protective roles in heart failure after myocardial infarction via suppression of the cytosolic JNK/p53 pathway in mice.2014

    • Author(s)
      Sun A, Zou Y, Wang P, Xu D, Gong H, Wang S, Qin Y, Zhang P, Chen Y, Harada M, Isse T, Kawamoto T, Fan H, Yang P, Akazawa H, Nagai T, Ping P, Komuro I, Ge J.
    • Journal Title

      J Am Heart Assoc

      Volume: 3 Issue: 5

    • DOI

      10.1161/jaha.113.000779

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Anti-inflammatory peptides from cardiac progenitors ameliorate dysfunction after myocardial infarction.2014

    • Author(s)
      Liu ML, Nagai T, Tokunaga M, Iwanaga K, Matsuura K, Takahashi T, Kanda M, Kondo N, Naito AT, Komuro I, Kobayashi Y.
    • Journal Title

      J Am Heart Assoc

      Volume: 3 Issue: 6

    • DOI

      10.1161/jaha.114.001101

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cardiac side population cells and Sca-1-positive cells2013

    • Author(s)
      Nagai T, Matsuura K, Komuro I.
    • Journal Title

      Methods Mol Biol

      Volume: 1036 Pages: 63-74

    • DOI

      10.1007/978-1-62703-511-8_5

    • ISBN
      9781627035101, 9781627035118
    • Related Report
      2013 Annual Research Report
  • [Journal Article] Notch activation mediates angiotensin II-induced vascular remodeling by promoting the proliferation and migration of vascular smooth muscle cells2013

    • Author(s)
      Ozasa Y, Akazawa H, Qin Y, Tateno K, Ito K, Kudo-Sakamoto Y, Yano M, Yabumoto C, Naito AT, Oka T, Lee JK, Minamino T, Nagai T, Kobayashi Y, Komuro I
    • Journal Title

      Hypertens Res

      Volume: 36(10) Issue: 10 Pages: 859-65

    • DOI

      10.1038/hr.2013.52

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] Novel Multicolor Lineage Tracing Model Can Detect Cardiomyocyte-Derived Cardiac Regeneration after Injury.2016

    • Author(s)
      Kanda M, Nagai T, Takahashi T, Liu M, Kondou N, Naito A, Akazawa H, Komuro I, Kobayashi Y.
    • Organizer
      第80回日本循環器学会総会・学術集会
    • Place of Presentation
      仙台国際センター, 宮城県, 仙台市
    • Year and Date
      2016-03-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] A Novel Multicolor Lineage Tracing Model Can Detect Cardiomyocyte-Derived Cardiac Regeneration After Myocardial Infarction.2015

    • Author(s)
      Kanda M, Nagai T, Takahashi T, Liu M, Kondou N, Naito A, Akazawa H, Komuro I, Kobayashi Y.
    • Organizer
      American Heart Association Scientific Sessions 2015
    • Place of Presentation
      Orlando, Florida, USA
    • Year and Date
      2015-11-07
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Leukemia Inhibitory Factor Enhances Cardiomyocyte Regeneration after Myocardial Infarction from Endogenous stem Cells and not from Circulating Bone Marrow-derived Cells.2015

    • Author(s)
      Kanda M, Nagai T, Takahashi T, Liu M, Kondou N, Naito A, Akazawa H, Komuro I, Kobayashi Y.
    • Organizer
      European Society of Cardiology Congress 2015
    • Place of Presentation
      London, UK
    • Year and Date
      2015-09-03
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 細胞動員の制御による心臓修復治療2014

    • Author(s)
      李 鍾國
    • Organizer
      第13回日本再生医療学会
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 心筋再生治療の可能性2013

    • Author(s)
      永井敏雄
    • Organizer
      第17回日本心不全学会
    • Place of Presentation
      大宮
    • Related Report
      2013 Annual Research Report
    • Invited

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Published: 2013-05-21   Modified: 2019-07-29  

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