| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2015: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2014: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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| Outline of Final Research Achievements |
Background During a 7-year period, we experienced four patients with slowly progressive encephalomyelitis in the same geographical area. Brain biopsy showed histological changes characteristic of non-viral encephalitis. We reported the clinical features of this encephalitis and the morphology of the microbe identified in brain biopsies with its molecular analysis.
Methods We extensively investigated four patients with unknown subacute/chronic dementia. Affected brain tissues were analyzed histopathologically, and DNA sequences of the responsible pathogen were detected using brain biopsy tissues.
Findings Histopathology of biopsied brain tissue revealed a microorganism was observed around the vessels in all patients. Ultrastructural analysis of this microorganism revealed the lack of a cell wall. Based on the morphology of the pathogen, we hypothesized that it belonged to Archaea. Next-generation sequencing (NGS) identified sequences were homologous to Halobacteriaceae.
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