Chromatin Dynamics and Epigenetic Regulation of Brown-Fat-Specific Transcription
| Project/Area Number |
25293209
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
WAKI HIRONORI 東京大学, 医学部附属病院, 特任准教授 (00466765)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2013: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Keywords | 褐色脂肪細胞 / 脂肪細胞 / エピゲノム / クロマチン / 遺伝子 / 転写制御 / 分化 / 肥満 / 褐色脂肪 / エンハンサー / 転写因子 |
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| Outline of Final Research Achievements |
To gain insight into the underlying mechanism of brown fat development, we performed formaldehyde-assisted isolation of regulatory elements analysis coupled with high-throughput sequencing (FAIRE-seq) of murine brown adipose tissue (BAT) and white adipose tissue (WAT). Through motif analysis of BAT-specific open chromatin regions and expression analysis, we identified NFIA as a novel regulator of brown adipocyte differentiation. Using cell culture, NFIA knockout mice, and chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq), we demonstrated that NFIA controls the BAT-specific gene program by activating the cell-type-specific enhancers and facilitating the binding of the adipogenic master regulator, PPARγ.
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Report
(4 results)
Research Products
(27 results)
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[Presentation] 褐色脂肪特異的な転写制御・エピゲノム制御におけるNFIAの役割の解明2015
Author(s)
脇裕典, 平池勇雄, 于静, 中村正裕, 孫威, 三宅加奈, 鈴木顕, 青山倫久, 冨岡恵, 廣田雄輔, 植木浩二郎, 堤修一, 油谷浩幸, 山内敏正, 門脇孝
Organizer
日本糖尿病・肥満動物学会
Place of Presentation
京都市
Year and Date
2015-02-14
Related Report
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[Presentation] Genome-wide Profiling of Brown Fat-Specific Open Regulatory Regions Identifies NFIA as a Transcriptional Regulator of Brown Fat Differentiation2014
Author(s)
Hironori Waki, Yuta Hiraike Jing Yu, Kana Miyake, Masahiro Nakamura, Ken Suzuki, Wei Sun, Megumi Tomioka, Yusuke Hirota, Tomohisa Aoyama, Masato Iwabu, Miki Okada-Iwabu, Kohjiro Ueki, Shuichi Tsutsumi, Hiroyuki Aburatani, Toshimasa Yamauchi and Takashi Kadowaki
Organizer
International Symposium for the Study of Obesity〝Molecular Basis and Disorders of Control of Appetite and Fat Accumulation″
Place of Presentation
Miyazaki, Japan
Year and Date
2014-10-26
Related Report
Invited
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[Presentation] Genome-wide Profiling of Brown Fat-Specific Open Regulatory Regions Identifies NFIA as a Transcriptional Regulator of Brown Fat Muscle Cell Lineage Specification2014
Author(s)
Hironori Waki, Yuta Hiraike, Jing Yu, Kana Miyake, Masahiro Nakamura, Ken Suzuki, Kohjiro Ueki, Shuichi Tsutsumi, Hiroyuki Aburatani, Toshimasa Yamauchi, Takashi Kadowaki
Organizer
9th Metabolic Syndrome, Type 2 Diabetes and Atherosclerosis Congress
Place of Presentation
Kyoto, Japan
Year and Date
2014-09-13
Related Report
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[Presentation] Genome-wide Profiling of Brown Fat-Specific Open Regulatory Regions Identifies NFIA as a Transcriptional Regulator of Brown Fat Muscle Cell Lineage Specification2014
Author(s)
Yuta Hiraike, Jing Yu, Hironori Waki, Masahiro Nakamura, Wei Sun, Tomohisa Aoyama, Megumi Tomioka, Masato Iwabu, Miki Okada-Iwabu, Kohjiro Ueki, Shuichi Tsutsumi, Hiroyuki Aburatani, Toshimasa Yamauchi, Takashi Kadowaki
Organizer
Exploring the Role of Brown Fat in Humans
Place of Presentation
NIH, Bethesda, MD, 米国
Related Report
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