| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2013: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Outline of Final Research Achievements |
To gain insight into the underlying mechanism of brown fat development, we performed formaldehyde-assisted isolation of regulatory elements analysis coupled with high-throughput sequencing (FAIRE-seq) of murine brown adipose tissue (BAT) and white adipose tissue (WAT). Through motif analysis of BAT-specific open chromatin regions and expression analysis, we identified NFIA as a novel regulator of brown adipocyte differentiation. Using cell culture, NFIA knockout mice, and chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq), we demonstrated that NFIA controls the BAT-specific gene program by activating the cell-type-specific enhancers and facilitating the binding of the adipogenic master regulator, PPARγ.
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