| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
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| Outline of Final Research Achievements |
We previously showed that multiple cytokines can directly stimulate long-term hematopoietic stem cells (LT-HSC), resulting in their self-renewal. However, whether different cytokines lead to the expansion of different types of HSC was not known. In this study, we focused on thrombopoietin (TPO) and interleukin-12 (IL-12), and compared their self-renewal-inducing effects using single-cell culture combined with transplantation assay. Data showed that the number of short term-HSC was significantly increased after the culture with stem cells factor (SCF)+TPO or SCF+IL-12. Data also suggested that some LT-HSCs were expanded after those cultures. On-going experiments will confirm these findings and also clarify their different effects.
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