Molecular Mechanisms of atherosclerotic remodeling in childhood

• Project/Area Number: 25293236
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Yokohama City University
• Principal Investigator: YOKOYAMA Utako 横浜市立大学, 医学部, 准教授 (70404994)
• Co-Investigator(Kenkyū-buntansha): 市川 泰広 横浜市立大学, 医学部, 助教 (10555121)
• Co-Investigator(Renkei-kenkyūsha): SUGIMOTO Yukihiko 熊本大学, 生命科学研究部, 教授 (80243038) ; NISHIHARA Hiroshi 北海道大学, 医学研究科, 特任准教授 (50322805) ; ARAKAWA Noriaki 横浜市立大学, 生命ナノシステム科学研究科, 助教 (60398394)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2013: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: 動脈硬化 / 小児 / 内膜肥厚 / リモデリング / 冠動脈 / プロスタグランディンE / 循環器・高血圧 / プロスタグランディン / 生理学

Outline of Final Research Achievements

The process of progression of atherosclerosis during childhood and its molecular mechanisms are not fully understood. We aimed to examine the developmental changes of atherosclerosis using vascular tissue samples ranging in age from 0 to 71 years old. In human coronary arterial smooth muscle cells, prostaglandin E receptor EP4 was highly expressed among EP receptors. Physiological intimal thickening was observed from newborn infants. We analyzed ApoE-/- EP4+/+ and ApoE-/- EP4+/- mice and found that EP4 deficiency inhibited atherosclerotic plaque formation. These data suggested that EP4 contributes to progression of atherosclerotic remodeling from childhood.

Research Products

• [Journal Article] Enhanced EP4 Expression in a Pulmonary Artery Aneurysm with Dissection in a Patient with Pulmonary Arterial Hypertension. (2017)
  • Author(s): Akagi S, Nakamura K, Yokoyama U, Kasahara S, Sarashina T1, Ejiri K, Sano S, Ito H.
  • Journal Title: Circ Cardiovasc Imaging Volume: 10 Pages: e005839
  • DOI: 10.1161/CIRCIMAGING.116.005839.
  • Peer Reviewed
• [Journal Article] Arterial graft with elastic layer structure grown from cells. (2017)
  • Author(s): Yokoyama U, Tonooka Y, Koretake R, Akimoto T, Gonda Y, Saito J, Umemura M, Fujita T, Sakuma S, Arai F, Kaneko M, Ishikawa Y.
  • Journal Title: Sci Rep Volume: 1 Pages: 140
  • DOI: 10.1038/s41598-017-00237-1
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Pathology and Molecular Mechanisms of Coarctation of the Aorta and its Association with the Ductus Arteriosus. (2017)
  • Author(s): Yokoyama U, Ichikawa Y, Minamisawa S, Ishikawa Y.
  • Journal Title: J Physiol Sci. Volume: 67 Pages: 259-270
  • DOI: 10.1007/s12576-016-0512-x
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Glutamate promotes contraction of the rat ductus arteriosus. (2016)
  • Author(s): Fujita S, Yokoyama U, Ishiwata R, Aoki R, Nagao K, Masukawa D, Umemura M, Fujita T, Iwasaki S, Nishimaki S, Seki K, Ito S, Goshima Y, Asou T, Masuda M, Ishikawa Y.
  • Journal Title: Circ J Volume: 80 Pages: 2388-2396
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Epac1 deficiency attenuated vascular smooth muscle cell migration and neointimal formation. (2015)
  • Author(s): Kato Y, Yokoyama U, Yanai C, Ishige R, Kurotaki D, Umemura M, Fujita T, Kubota T, Okumura S, Sata M, Tamura T, Ishikawa Y.
  • Journal Title: Arterioscler Thromb Vasc Biol. Volume: 35(12) Pages: 2617-2625
  • DOI: 10.1161/ATVBAHA.115.306534.
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Prostaglandin E-mediated molecular mechanisms driving remodeling of the ductus arteriosus. (2015)
  • Author(s): Yokoyama U.
  • Journal Title: Pediatr Int. Volume: 57(5) Pages: 820-827
  • DOI: 10.1111/ped.12769.
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Norepinephrine-induced adrenergic activation strikingly increased the atrial fibrillation duration through β1- and α1-adrenergic receptor-mediated signaling in mice. (2015)
  • Author(s): Suita K, Fujita T, Hasegawa N, Cai W, Jin H, Hidaka Y, Prajapati R, Umemura M, Yokoyama U, Sato M, Okumura S, Ishikawa Y.
  • Journal Title: PLoS One. Volume: 23;10(7) Pages: e0133664
  • DOI: 10.1371/journal.pone.0133664.
  • Peer Reviewed / Open Access
• [Journal Article] Coupling of beta1-adrenergic receptor to type 5 adenylyl cyclase and its physiological relevance in cardiac myocytes. (2015)
  • Author(s): TsunematsuT, Okumura S, Mototani Y, Ohnuki Y, Jin H, Cai W, Suita K, Sato I, Umemura M, Yokoyama U, Sato M, Fujita T, Ishikawa Y.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 458(3) Pages: 531-535
  • DOI: 10.1016/j.bbrc.2015.01.149.
  • Peer Reviewed / Open Access
• [Journal Article] Oscillation of cAMP and Ca2+ in cardiac myocytes: a systems biology approach. (2015)
  • Author(s): Kamide T, Okumura S, Ghosh S, Shinoda Y, Mototani Y, Ohnuki Y, Jin H, Cai W, Suita K, Sato I, Umemura M, Fujita T, Yokoyama U, Sato M, Furutani K, Kitano H, Ishikawa Y.
  • Journal Title: J Physiol Sci. Volume: 65(2) Pages: 195-200
  • DOI: 10.1007/s12576-014-0354-3.
  • Peer Reviewed
• [Journal Article] Coupling of beta1-adrenergic receptor to type 5 adenylyl cyclase and its physiological relevance in cardiac myocytes. (2015)
  • Author(s): TsunematsuT, Okumura S, Mototani Y, Ohnuki Y, Jin H, Cai W, Suita K, Sato I, Umemura M, Yokoyama U, Sato M, Fujita T, Ishikawa Y.
  • Journal Title: Biochemical and Biophysical Research Communications (BBRC) Volume: - Pages: -
  • DOI: -
  • Peer Reviewed / Open Access
• [Journal Article] Microfluidic perfusion culture system for multilayer artery tissue models. (2014)
  • Author(s): Yamagishi Y, Masuda T, Matsusaki M, Akashi M, Yokoyama U, Arai F.
  • Journal Title: Biomicrofluidics. Volume: VOL.8 Pages: 064113
  • DOI: 10.1063/1.4903210
  • Peer Reviewed / Open Access
• [Journal Article] Exendin-4 ameliorates cardiac ischemia/reperfusion injury via caveolae and caveolins-3. (2014)
  • Author(s): Tsutsumi YM, Tsutsumi R, Hamaguchi E, Sakai Y, Kasai A, Ishikawa Y, Yokoyama U, Tanaka K.
  • Journal Title: Cardiovasc Diabetol. Volume: VOL.13 Pages: 132
  • DOI: 10.1186/PREACCEPT-1916507751134656
  • Peer Reviewed / Open Access
• [Journal Article] Decreased serum osmolality promotes ductus arteriosus constriction. (2014)
  • Author(s): Aoki R, Yokoyama U*, Ichikawa Y, Taguri M, Kumagaya S, Ishiwata R, Yanai C, Fujita S, Umemura M, Fujita T, Okumura S, Sato M, Minamisawa S, Asou T, Masuda M, Iwasaki S, Nishimaki S, Seki K, Yokota S, Ishikawa Y.
  • Journal Title: Cardiovasc Res. Volume: VOL.104 Pages: 326～336
  • DOI: 10.1093/cvr/cvu199
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Epac1-dependent phospholamban phosphorylation mediates the cardiac response to stresses. (2014)
  • Author(s): Okumura S, Fujita T, Cai W, Jin M, Namekata I, Mototani Y, Jin H, Ohnuki Y, Tsuneoka Y, Kurotani R, Suita K, Kawakami Y, Hamaguchi S, Abe T, Kiyonari H, Tsunematsu T, Bai Y, Suzuki S, Hidaka Y, Umemura M, Ichikawa Y, Yokoyama U, Sato M, Ishikawa F, Izumi-Nakaseko H, Adachi-Akahane S, Tanaka H, Ishikawa Y.
  • Journal Title: J Clin Invest. Volume: VOL.124 Pages: 2785～2801
  • DOI: 10.1172/JCI64784
  • Peer Reviewed / Open Access
• [Journal Article] Thromboxane A(2) receptor stimulation promotes closure of the rat ductus arteriosus through enhancing neointima formation. (2014)
  • Author(s): Yokota T, Shiraishi R, Aida T, Iwai K, Liu NM, Yokoyama U, Minamisawa S.
  • Journal Title: PLoS One. Volume: VOL.9 Pages: e94895
  • DOI: 10.1371/journal.pone.0094895
  • Peer Reviewed / Open Access
• [Journal Article] Protection of cardiomyocytes from the hypoxia-mediated injury by a Peptide targeting the activator of g-protein signaling 8. (2014)
  • Author(s): Sato M, Hiraoka M, Suzuki H, Sakima M, Mamun AA, Yamane Y, Fujita T, Yokoyama U, Okumura S, Ishikawa Y.
  • Journal Title: PLoS One. Volume: VOL.9 Pages: e91980
  • DOI: 10.1371/journal.pone.0091980
  • Peer Reviewed / Open Access
• [Journal Article] Hyperthermia generated with ferucarbotran (Resovist®) in an alternating magnetic field enhances cisplatin-induced apoptosis of cultured human oral cancer cells. (2014)
  • Author(s): Sato I, Umemura M, Mitsudo K, Kioi M, Nakashima H, Iwai T, Feng X, Oda K, Miyajima A, Makino A, Iwai M, Fujita T, Yokoyama U, Okumura S, Sato M, Eguchi H, Tohnai I, Ishikawa Y.
  • Journal Title: J Physiol Sci. Volume: VOL.64 Pages: 177～183
  • DOI: 10.1007/s12576-014-0309-8
  • Peer Reviewed / Open Access
• [Journal Article] Store-Operated Ca(2) (+) Entry (SOCE) Regulates Melanoma Proliferation and Cell Migration. (2014)
  • Author(s): Umemura M, Baljinnyam E, Feske S, De Lorenzo MS, Xie LH, Feng X, Oda K, Makino A, Fujita T, Yokoyama U, Iwatsubo M, Chen S, Goydos JS, Ishikawa Y, Iwatsubo K.
  • Journal Title: PLoS One. Volume: VOL.9 Pages: e89292
  • DOI: 10.1371/journal.pone.0089292
  • Peer Reviewed / Open Access
• [Journal Article] Geranylgeranylacetone protects the heart via caveolae and caveolin-3. (2014)
  • Author(s): Tsutsumi YM, Tsutsumi R, Horikawa YT, Sakai Y, Hamaguchi E, Ishikawa Y, Yokoyama U, Kasai A, Kambe N, Tanaka K.
  • Journal Title: Life Sci, Volume: VOL.101 Pages: 43～48
  • DOI: 10.1016/j.lfs.2014.02.019
  • Peer Reviewed / Open Access
• [Journal Article] Three-Dimensional Multilayers of Smooth Muscle Cells as a New Experimental Model for Vascular Elastic Fiber Formation Studies. (2014)
  • Author(s): Ishiwata R, Yokoyama U*, Matsusaki M, Asano Y, Kadowaki K, Ichikawa Y, Umemura M, Fujita T, Minamisawa S, Shimoda H, Akashi M, and Ishikawa Y.
  • Journal Title: Atherosclerosis, Volume: VOL.233 Pages: 590～600
  • DOI: 10.1016/j.atherosclerosis.2014.01.045
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Prostaglandin E2 inhibits elastogenesis in the ductus arteriosus via EP4 signaling. (2014)
  • Author(s): Yokoyama U, Minamisawa S, Shioda A, Ishiwata R, Jin MH, Masuda M, Asou T, Sugimoto Y, Aoki H, Nakamura T, Ishikawa Y.
  • Journal Title: Circulation, Volume: VOL.129 Pages: 487～496
  • DOI: 10.1161/CIRCULATIONAHA.113.004726
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Prostaglandin E2 inhibits elastogenesis in the ductus arteriosus via EP4 signaling (2014)
  • Author(s): Yokoyama U, Minamisawa S, Shioda A, Ishiwata R, Jin MH, Masuda M, Asou T, Sugimoto Y, Aoki H, Nakamura T, Ishikawa Y
  • Journal Title: Circulation Volume: 129 Pages: 487-96
  • DOI: 10.1161/CIRCULATIONAHA.113.004726
  • Peer Reviewed
• [Journal Article] 動脈管閉鎖の分子メカニズムと新たな薬物療法の可能性 (2014)
  • Author(s): 横山詩子
  • Journal Title: 小児科 Volume: 55 Pages: 11-20
• [Journal Article] The prostanoid EP4 receptor and its signaling pathway (2013)
  • Author(s): Yokoyama U, Iwatsubo K, Umemura M, Fujita T, Ishikawa Y
  • Journal Title: Pharmacol Rev Volume: 65 Pages: 1010-52
  • DOI: 10.1124/pr.112.007195
  • Peer Reviewed
• [Journal Article] 動脈硬化・動脈瘤病態形成におけるプロスタグランディンE2の役割（The role of prostaglandin E2 in atherosclerosis and aortic aneurysm） (2013)
  • Author(s): 横山詩子
  • Journal Title: 血栓と循環 Volume: 21 Pages: 29-33
• [Presentation] Oxygenation-induced postnatal remodeling of the ductus arteriosus. (2017)
  • Author(s): Jin MH, Yokoyama U, Ishiwata R, Minamisawa S, Ishikawa Y.
  • Organizer: The 94th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 浜松アクトシティコングレスセンター（静岡県浜松市）
  • Year and Date: 2017-03-28 – 2017-03-30
  • Invited
• [Presentation] Human gene analysis identified tissue plasminogen activator as a mediator of disrupting the internal elastic lamina in the ductus arteriosus. (2017)
  • Author(s): Saito J, Yokoyama U, Ishikawa Y.
  • Organizer: The 94th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 浜松アクトシティコングレスセンター（静岡県浜松市）
  • Year and Date: 2017-03-28 – 2017-03-30
• [Presentation] An EP4 antagonist has inhibitory and therapeutic effects on mouse models of abdominal aortic aneurysm. (2017)
  • Author(s): Al Mamun, Yokoyama U, Ishiwata R, Isono O, Nagabukuro H, Ishikawa Y.
  • Organizer: The 94th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 浜松アクトシティコングレスセンター（静岡県浜松市）
  • Year and Date: 2017-03-28 – 2017-03-30
• [Presentation] Clinical Course of Pulmonary Artery Aneurysm in Pulmonary Hypertension: Insight into Medical Treatment and Histological Characteristics (2017)
  • Author(s): Akagi S, Nakamura K, Yokoyama U, Sarashina T, Ejiri K, Itoh H.
  • Organizer: The 81th Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: Ishikawa Ongakudo, Kanazawa
  • Year and Date: 2017-03-17 – 2017-03-19
• [Presentation] Fibulin-1 は平滑筋細胞遊走を介して動脈管内膜肥厚を誘導する (2016)
  • Author(s): 伊藤智子, 横山詩子, 益田宗孝, 麻生俊英, 石川義弘
  • Organizer: 第６１回日本新生児成育医学会学術集会
  • Place of Presentation: 大阪国際会議場（大阪府大阪市）
  • Year and Date: 2016-12-01 – 2016-12-03
• [Presentation] EP4 signaling in smooth muscle cells attracts inflammatory immune responses in the aorta. (2016)
  • Author(s): Ishiwata R, Yokoyama U, Ichikawa Y, Kurotaki D, Yasuda S, Goda M, Suzuki S, Masuda M, Tamura T, Ishikawa Y.
  • Organizer: The 89th Scientific Session, American Heart Association
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2016-11-12 – 2016-11-16
  • Int'l Joint Research
• [Presentation] Human Gene Profiling Reveals contribution of Tissue Plasminogen Activator to Intimal Thickening of the Ductus Arteriosus. (2016)
  • Author(s): Saito J, Yokoyama U, Masuda M, Asou T, Ishikawa Y.
  • Organizer: The 89th Scientific Session, American Heart Association
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2016-11-12 – 2016-11-16
  • Int'l Joint Research
• [Presentation] Fibulin-1 plays a role in smooth muscle cell migration and intimal thickening in the ductus arteriosus. (2016)
  • Author(s): Ito S, Yokoyama U, Yanai C, Masuda M, Asou T, Ishikawa Y.
  • Organizer: The 89th Scientific Session, American Heart Association
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2016-11-12 – 2016-11-16
  • Int'l Joint Research
• [Presentation] 組織型プラスミノーゲン活性化因子による動脈管内弾性板の分解 (2016)
  • Author(s): 齋藤純一, 横山詩子, 石川義弘
  • Organizer: 第15回日本心臓血管発生研究会
  • Place of Presentation: 国立循環器病研究センター（大阪府吹田市）
  • Year and Date: 2016-10-14 – 2016-10-15
• [Presentation] PGE2-EP4シグナルはfibulin-1を介して動脈管平滑筋遊走を促進する (2016)
  • Author(s): 伊藤智子, 横山詩子, 石川義弘
  • Organizer: 第15回日本心臓血管発生研究会
  • Place of Presentation: 国立循環器病研究センター（大阪府吹田市）
  • Year and Date: 2016-10-14 – 2016-10-15
• [Presentation] 動脈硬化性疾患に対する薬物治療の開発 (2016)
  • Author(s): 横山詩子
  • Organizer: Atherosclerosis and Angiogenesis Research Frontier
  • Place of Presentation: 旭川グランドホテル（北海道旭川市）
  • Year and Date: 2016-08-26 – 2016-08-26
  • Invited
• [Presentation] ヒト動脈管における内膜肥厚部の遺伝子プロファイリング (2016)
  • Author(s): 齋藤純一，横山詩子，麻生俊英，石川義弘
  • Organizer: 第52回日本周産期・新生児医学会学術集会
  • Place of Presentation: 富山国際会議場（富山県富山市）
  • Year and Date: 2016-07-16 – 2016-07-18
• [Presentation] 平滑筋細胞におけるPGE2-EP4シグナルは腹部大動脈瘤の発症を促す (2016)
  • Author(s): 石渡 遼, 横山 詩子, 市川 泰弘, 黒滝 大翼, 田村 智彦, 石川 義弘
  • Organizer: 第３７回日本循環制御医学会総会
  • Place of Presentation: ステーションコンファレンス東京（東京都）
  • Year and Date: 2016-07-08 – 2016-07-09
• [Presentation] The role of Epac1 in the regulatory mechanisms of vascular smooth muscle cell migration and neointimal formation. (2016)
  • Author(s): Kato Y, Yokoyama U, Ishikawa Y.
  • Organizer: The 93th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市白石区）
  • Year and Date: 2016-03-22 – 2016-03-24
• [Presentation] Periodic hydrodynamic pressurization promotes layered structure of human arterial smooth muscle cells. (2016)
  • Author(s): Tonooka Y, Yokoyama U, Koretake R, Sakuma S, Kaneko M, Ishikawa Y.
  • Organizer: The 93th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市白石区）
  • Year and Date: 2016-03-22 – 2016-03-24
• [Presentation] Fabrication of layered elastic fibers in rat arterial constructs by periodic hydrodynamic pressurization. (2016)
  • Author(s): Gonda Y, Yokoyama U, Koretake R, Sakuma S, Kaneko M, Ishikawa Y.
  • Organizer: The 93th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市白石区）
  • Year and Date: 2016-03-22 – 2016-03-24
• [Presentation] 血管平滑筋と細胞外基質制御における新たなホスホリパーゼCとサイクリックAMPの役割The roles of phospholipase C and cyclic AMP in regulation of extracellular matrix in vascular smooth muscle cells. (2016)
  • Author(s): Yokoyama U.
  • Organizer: The 93th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市白石区）
  • Year and Date: 2016-03-22 – 2016-03-24
  • Invited
• [Presentation] Inhibition of PGE2-EP4 Signaling as a PotentialFuture Therapeutic Strategy for Abdominal Aortic Aneurysm. (2016)
  • Author(s): Yokoyama U, Ishiwata R, Ichikawa Y, Yasuda S, Goda M, Suzuki S, Masuda M, Ishikawa Y.
  • Organizer: The 80th Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: 仙台国際センター（宮城県仙台市青葉区）
  • Year and Date: 2016-03-18 – 2016-03-20
  • Invited
• [Presentation] 早産児の動脈管はなぜ閉じないのか (2015)
  • Author(s): 横山詩子
  • Organizer: 第２８回北海道新生児談話会
  • Place of Presentation: 北海道大学病院（北海道札幌市北区）
  • Year and Date: 2015-11-15
  • Invited
• [Presentation] 動脈管閉鎖の分子機序と新たな薬物療法の可能性 (2015)
  • Author(s): 横山詩子
  • Organizer: 第２４回東海新生児研究会
  • Place of Presentation: 名古屋市立大学病院（愛知県名古屋市瑞穂区）
  • Year and Date: 2015-11-14
  • Invited
• [Presentation] プロスタグランディンE受容体―EP4を標的とした大動脈瘤治療の開発 (2015)
  • Author(s): 横山詩子
  • Organizer: 岡山心臓血管研究会
  • Place of Presentation: 岡山大学病院（岡山県岡山市北区）
  • Year and Date: 2015-09-30
  • Invited
• [Presentation] 平滑筋におけるPGE2-EP4シグナルは腹部大動脈瘤の慢性炎症をつかさどる (2015)
  • Author(s): 横山詩子、石渡遼、石川義弘
  • Organizer: 第２４６回生理学東京談話会
  • Place of Presentation: 東邦大学医学部（東京都大田区）
  • Year and Date: 2015-09-26
• [Presentation] 未熟児動脈管開存症治療におけるアミノ酸輸液組成の重要性 The effect of amino acids on patent ductus arteriosus in preterm infants (2015)
  • Author(s): 横山詩子、藤田秀次郎、青木理加、岩崎志穂、麻生俊英、益田宗孝、関和男、石川義弘
  • Organizer: 第51回日本小児循環器学会総会・学術集会
  • Place of Presentation: ホテル日航東京（東京都港区）
  • Year and Date: 2015-07-16 – 2015-07-18
• [Presentation] ヒト動脈管における内膜肥厚部の遺伝子プロファイリングGene profiling of the intimal thickness in the human ductus arteriosus (2015)
  • Author(s): 齋藤純一、横山詩子、市川泰広、益田宗孝、麻生俊英、石川義弘
  • Organizer: 第51回日本小児循環器学会総会・学術集会
  • Place of Presentation: ホテル日航東京（東京都港区）
  • Year and Date: 2015-07-16 – 2015-07-18
• [Presentation] 動脈管分化の分子メカニズム (2015)
  • Author(s): 横山詩子
  • Organizer: 第51回日本小児循環器学会総会・学術集会
  • Place of Presentation: ホテル日航東京（東京都港区）
  • Year and Date: 2015-07-16 – 2015-07-18
  • Invited
• [Presentation] 平滑筋におけるPGE2-EP4シグナルは腹部大動脈瘤の発症を促進する (2015)
  • Author(s): 石渡遼、横山詩子、市川泰弘、石川義弘
  • Organizer: 第132回日本薬理学会関東部会
  • Place of Presentation: 明海大学浦安キャンパス（千葉県浦安市）
  • Year and Date: 2015-07-04
• [Presentation] 動脈管開存症治療における輸液組成の重要性 (2015)
  • Author(s): 横山詩子
  • Organizer: 神奈川県立こども医療センター第２４回心臓血管外科勉強会
  • Place of Presentation: 神奈川県立こども医療センター（神奈川県横浜市南区）
  • Year and Date: 2015-06-12
  • Invited
• [Presentation] Regulation of collagens in the heart. (2015)
  • Author(s): Yokoyama U.
  • Organizer: The 79th Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: 大阪国際会議場（大阪府大阪市北区）
  • Year and Date: 2015-04-25
  • Invited
• [Presentation] Plasminogen activator may promote intimal thickening in the ductus arteriosus. (2015)
  • Author(s): Nicho N, Yokoyama U, Saitoh J, Yanai C, Ichikawa Y, Masuda M, Aso T, Ishikawa Y.
  • Organizer: The 118th Annual Meeting of the Japan Pediatric Society
  • Place of Presentation: 大阪国際会議場（大阪府大阪市北区）
  • Year and Date: 2015-04-17 – 2015-04-19
• [Presentation] 実験的アプローチによる新たな動脈管開存症治療の開発 (2015)
  • Author(s): 横山詩子
  • Organizer: 第一回新生児科指導医養成事業による教育セミナー
  • Place of Presentation: 東北大学病院（宮城県仙台市）
  • Year and Date: 2015-03-28 – 2015-03-28
  • Invited
• [Presentation] Fabrication of tissue-engineered human arterial constructs by cyclic hydrostatic pressure. (2015)
  • Author(s): Tonooka Y, Yokoyama U, Koretake R, Ishiwata R, Sakuma S, Kaneko M, Ishikawa Y.
  • Organizer: The 92th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 神戸国際会議場・展示場（兵庫県神戸市）
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] ナノ薄膜技術を用いた高弾性を有する動脈グラフトの作製 Construction of biological elastic vessels by extracellular matrix nanofilm-based cell accumulation technique. (2015)
  • Author(s): Yokoyama U.
  • Organizer: The 92th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 神戸国際会議場・展示場（兵庫県神戸市）
  • Year and Date: 2015-03-21 – 2015-03-23
  • Invited
• [Presentation] PGE2-EP4 sigaling promotes vascular intimal thickening via fibulin-1. (2015)
  • Author(s): Yokoyama U, Yanai C, Sato A, Ishikawa Y.
  • Organizer: The 6th International Conference on Phospholipase A2 and Lipid Mediators
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Year and Date: 2015-02-10 – 2015-02-12
• [Presentation] Secretome-based identification of PGE2-regulated proteins in human abdominal aortic aneurysm. (2015)
  • Author(s): Ishiwata R, Yokoyama U, Arakawa N, Ishikawa Y.
  • Organizer: he 6th International Conference on Phospholipase A2 and Lipid Mediators
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Year and Date: 2015-02-10 – 2015-02-12
• [Presentation] PGE2-EP4 exerts an anti-fibrotic effect in the heart. (2015)
  • Author(s): Ishiwata R. Yokoyama U, Inoue S, Ichikawa Y, Ishikawa Y.
  • Organizer: The 6th International Conference on Phospholipase A2 and Lipid Mediators
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Year and Date: 2015-02-10 – 2015-02-12
• [Presentation] PGE2-EP4 sigaling in smooth muscle cell plays a role in chronic inflammation of aortic abdominal aneurysm. (2015)
  • Author(s): Yokoyama U, Ichikawa Y, Ishiwata R, Ishikawa Y.
  • Organizer: The 6th International Conference on Phospholipase A2 and Lipid Mediators
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Year and Date: 2015-02-10 – 2015-02-12
  • Invited
• [Presentation] Prostaglandin E2 receptor EP4 signaling in vascular smooth muscle cells decreases aortic elasticity (2014)
  • Author(s): Ichikawa Y, Yokoyama U, Ishikawa Y
  • Organizer: Experimental Biology Annual Meeting
  • Place of Presentation: USA San Diego Convention center
  • Year and Date: 20140426-20140430
• [Presentation] Prostaglandin E2 Receptor EP4 Signaling in Vascular Smooth Muscle Plays a Role in Arterial Elasticity (2014)
  • Author(s): Ichikawa Y, Yokoyama U, Minamisawa S, Ishikawa Y
  • Organizer: The78th Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: 東京国際フォーラム（東京都千代田区）
  • Year and Date: 20140321-20140323
• [Presentation] PGE2-EP4 signaling is a key regulator of cardiovascular stiffness and elasticity (2014)
  • Author(s): Yokoyama U
  • Organizer: The 91th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島県鹿児島市）
  • Year and Date: 20140316-20140318
  • Invited
• [Presentation] Prostaglandin E2-EP4 signaling-induced fibulin-1 may play a role in intimal thickening in the ductus arteriosus (2014)
  • Author(s): Kumagaya S, Yokoyama U, Minamisawa S, Inoue T, Ishikawa Y
  • Organizer: The 91th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島県鹿児島市）
  • Year and Date: 20140316-20140318
• [Presentation] Prostaglandin E2 Receptor EP4 Signaling in Smooth Muscle Cells Regulates Arterial Elasticity (2014)
  • Author(s): Ichikawa Y, Yokoyama U, Ishikawa Y
  • Organizer: The 91th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島県鹿児島市）
  • Year and Date: 20140316-20140318
• [Presentation] プロスタグランディンE－EP4はfibulin-1を介して生理的・病的血管内膜肥厚の形成に関与する Prostaglandin E2-EP4 is involved in physiological and pathological intimal thickening via fibulin-1 (2014)
  • Author(s): 矢内千春、横山詩子、熊谷駿、手島隆之、佐藤綾美、石川義弘
  • Organizer: 第13回日本心臓血管発生研究会
  • Place of Presentation: 磐梯熱海温泉 ホテル 華の湯（福島県郡山市）
  • Year and Date: 2014-10-17 – 2014-10-18
• [Presentation] Prostaglandin E2-EP4 plays a protective role against cardiac fibrosis. (2014)
  • Author(s): Ishiwata R, Yokoyama U, Inoue S, Ichikawa Y, Ishikawa Y.
  • Organizer: European Society of Cardiology
  • Place of Presentation: Spain, Barcelona
  • Year and Date: 2014-08-30 – 2014-09-03
• [Presentation] 動脈管は面白い (2014)
  • Author(s): 横山詩子
  • Organizer: 第24回心研小児科夏季セミナー
  • Place of Presentation: 東京女子医科大学（東京都新宿区）
  • Year and Date: 2014-07-26 – 2014-07-27
  • Invited
• [Presentation] 仕事の多様化について (2014)
  • Author(s): 横山詩子
  • Organizer: 横浜市立大学第２回Joinの会
  • Place of Presentation: 横浜市立大学附属 市民総合医療センター(神奈川県横浜市)
  • Year and Date: 2014-07-19 – 2014-07-19
  • Invited
• [Presentation] プロスタグランディンEによる血管弾性線維の制御メカニズム (2014)
  • Author(s): 横山詩子
  • Organizer: 東京農工大学 材料健康科学「Cell to Body Dynamicsセミナー」
  • Place of Presentation: 東京農工大学（東京都小金井市）
  • Year and Date: 2014-07-18 – 2014-07-18
  • Invited
• [Presentation] グルタミン酸が動脈管閉鎖に及ぼす作用の検討 (2014)
  • Author(s): 藤田秀次郎、横山詩子、青木理加、岩崎志穂、西巻滋、関和男、横田俊平、石川義弘
  • Organizer: 第50回日本周産期・新生児医学会学術集会
  • Place of Presentation: シェラトングランデトーキョーベイ（千葉県浦安市）
  • Year and Date: 2014-07-13 – 2014-07-15
• [Presentation] ヒト大動脈瘤組織におけるPGE2-EP4誘導性分泌タンパク質の網羅的解析 (2014)
  • Author(s): 石渡遼、横山詩子、荒川憲昭、安田章沢、郷田素彦、鈴木伸一、益田宗孝、石川義弘
  • Organizer: 第35回日本循環制御医学会総会
  • Place of Presentation: 九州大学医学部百年講堂（福岡県福岡市）
  • Year and Date: 2014-07-04 – 2014-07-05
• [Presentation] Nuclear Factor kappa B Inhibition Promotes Closure of the Rat Ductus Arteriosus. (2014)
  • Author(s): Kajimura I, Yokoyama U, Ishikawa Y, Minamisawa S.
  • Organizer: Experimental Biology Annual Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2014-04-26 – 2014-04-30
• [Presentation] cAMP reverses the pro-fibrogenic phenotype of cardiac myofibroblasts. (2014)
  • Author(s): Aroonsakool N, Yokoyama U, Lu D, Patel HH, Insel PA.
  • Organizer: Experimental Biology Annual Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2014-04-26 – 2014-04-30
• [Presentation] Prostaglandin E2 receptor EP4 signaling in vascular smooth muscle cells decreases aortic elasticity. (2014)
  • Author(s): Ichikawa Y, Yokoyama U, Ishikawa Y.
  • Organizer: Experimental Biology Annual Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2014-04-26 – 2014-04-30
• [Presentation] Prostaglandin E2 Receptor EP4 Signaling in Vascular Smooth Muscle Decreased Elasticity of the Aorta (2013)
  • Author(s): Ichikawa Y, Yokoyama U, Iwamoto M, Yokota S, Minamisawa S, Ishikawa Y
  • Organizer: The 86th Scientific Session, American Heart Association
  • Place of Presentation: USA Dallas Convention center
  • Year and Date: 20131116-20131120
• [Presentation] 血管弾性線維におけるプロスタグランディンE受容体EP4の役割の検討 (2013)
  • Author(s): 市川泰広、横山詩子、南沢享、石川義弘
  • Organizer: 第２１回 日本血管生物医学会学術集会
  • Place of Presentation: 千里阪急ホテル（大阪府豊中市）
  • Year and Date: 20130926-20130928
• [Presentation] The multiple roles of prostaglandin E and oxygen in the regulation of the ductus arteriosus (2013)
  • Author(s): Yokoyama U, Jin MH, Ishiwata R, Ichikawa Y, Minamisawa S, Ishikawa Y
  • Organizer: The 7th TAKAO International symposium
  • Place of Presentation: 国立オリンピック記念青少年総合センター（東京都渋谷区）
  • Year and Date: 20130713-20130715
  • Invited
• [Presentation] Prostaglandin E2-EP4 signaling promotes secretion of the lipid-relating proteoglycan biglycan in human coronary arteries during development (2013)
  • Author(s): Kumagaya S, Yokoyama U, Sato A, Nishihara H, Inoue T, Minamisawa S, Ishikawa Y
  • Organizer: The 7th TAKAO International symposium
  • Place of Presentation: 国立オリンピック記念青少年総合センター（東京都渋谷区）
  • Year and Date: 20130713-20130715
• [Presentation] Prostaglandin E2-EP4 signaling inhibits vascular elastic fiber formation in the ductus arteriosus (2013)
  • Author(s): Ishiwata R, Yokoyama U, Jin MH, Masuda M, Asou T, Sugimoto Y, Aoki H, Nakamura T, Minamisawa S, Ishikawa Y
  • Organizer: The 7th TAKAO International symposium
  • Place of Presentation: 国立オリンピック記念青少年総合センター（東京都渋谷区）
  • Year and Date: 20130713-20130715
• [Presentation] Mouse Overexpressing Prostaglandin E Receptor EP4 in Vascular Smooth Muscle Cells Decreased Elasticity of the Aorta (2013)
  • Author(s): Ichikawa Y, Yokoyama U, Jin MH, Ishikawa Y
  • Organizer: The 7th TAKAO International symposium
  • Place of Presentation: 国立オリンピック記念青少年総合センター（東京都渋谷区）
  • Year and Date: 20130713-20130715
• [Book] Bioactive Lipid Mediators. Current Reviews and Protocols. (2015)
  • Author(s): Yokoyama U, Ishiwata R, Ishikawa Y.
  • Total Pages: 11
  • Publisher: Springer
• [Book] Etiology and Morphogenesis of Congenital Heart Disease. (2015)
  • Author(s): Yokoyama U, Minamisawa S, Ishikawa Y.
  • Total Pages: -
  • Publisher: Springer
• [Book] Nanomedicine and Nanotoxicology. (2014)
  • Author(s): Yokoyama U*, Ishikawa Y.
  • Total Pages: 271(159-174)
  • Publisher: Springer
• [Book] Etiology and Morphogenesis of Congenital Heart Disease (2014)
  • Author(s): Yokoyama U, Minamisawa S, Ishikawa Y
  • Total Pages: 5
  • Publisher: Springer
• [Remarks] 横浜市立大学医学部循環制御医学
  • URL: http://www-user.yokohama-cu.ac.jp/~seiri1/
• [Remarks] 横浜市立大学医学部循環制御医学
  • URL: http://www-user.yokohama-cu.ac.jp/~seiri1/index.html
• [Remarks] 横浜市立大学医学部循環制御医学
  • URL: http://www-user.yokohama-cu.ac.jp/~seiri1/
• [Patent(Industrial Property Rights)] 動脈硬化を検出するための血中バイオマーカー (2017)
  • Inventor(s): 横山詩子、石川義弘、ほか
  • Industrial Property Rights Holder: 横山詩子、石川義弘、ほか
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: PCT/JP2017/5565
  • Filing Date: 2017-02-16
  • Application Year: 2017
  • Overseas