| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
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| Outline of Final Research Achievements |
CNS-targeted conditional Aspm-knockout (KO) mice were generated to investigate the significance of Aspm in developing brain from the viewpoint of cell kinetics and the formation of cortical structure. We first studied whether the cell number showing S-phase as well as M-phase may be altered in Aspm-KO mice and found that no increase or decrease in the number of cells assigned to either S- or M-phase was demonstrated in the forebrain from embryonic day (E) 12.5 through 16.5. EdU-pulse labelling studies revealed that the labeled cells were decreased in the intermediate zone of Aspm-KO mice, indicating the significant reduction of newly-born neurons after E14.5. MRI imaging studies of postnatal Aspm-KO mice demonstrated the significant alterations in FA value (calculated from the tensor imaging) in the KO cerebral cortex when compared to wild type cerebral cortex, which is likely to correlate with the changes of the neurite direction shown in histo-morphometric studies.
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