| Project/Area Number |
25293246
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Kyorin University (2015)
Keio University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MASAYUKI AMAGAI 慶應義塾大学, 医学部, 教授 (90212563)
AKIHARU KUBO 慶應義塾大学, 医学部, 講師 (70335256)
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| Co-Investigator(Renkei-kenkyūsha) |
HIDEYUKI OKANO 慶應義塾大学, 医学部, 教授 (60160694)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Keywords | 毛包再生 / ヒトiPS細胞 / 分化誘導 / 上皮ー間葉系相互作用 / 上皮―間葉系相互作用 / 再生医学 / ヒト毛包再生 / 細胞・組織 |
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| Outline of Final Research Achievements |
The aim of this study is to generate hair follicle structure using human induced pluripotent stem cell (iPSC)-derived epithelial and mesenchymal components optimized for trichogenicity. With regards to epithelial cells, a stable protocol to induce keratinocytes containing stem cell marker expressing cells was established. For trichogenic mesenchymal component preparation, an approach to generate multipotent mesenchymal stem cell-like cells was developed. The resultant cells are further programmed to acquire hair inductive property of dermal papilla cells. When co-grafted with normal human keratinocytes into immunedeficient mice, those cells gave rise to hair follicle-like structures with moderate reconstitution efficiency. However, regeneration of hair follicle structure exclusively consisted of human iPSC-derived cells is still technically very challenging, mainly because of relatively low efficiency and adjustment of the timing in the production of both iPSC-derived subsets.
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