Attempt to regenerate hair follicles using human iPS cell-derived epithelial and mesenchymal cells optimized for trichogenicity
Project/Area Number |
25293246
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Dermatology
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Research Institution | Kyorin University (2015) Keio University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
MASAYUKI AMAGAI 慶應義塾大学, 医学部, 教授 (90212563)
AKIHARU KUBO 慶應義塾大学, 医学部, 講師 (70335256)
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Co-Investigator(Renkei-kenkyūsha) |
HIDEYUKI OKANO 慶應義塾大学, 医学部, 教授 (60160694)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
|
Keywords | 毛包再生 / ヒトiPS細胞 / 分化誘導 / 上皮ー間葉系相互作用 / 上皮―間葉系相互作用 / 再生医学 / ヒト毛包再生 / 細胞・組織 |
Outline of Final Research Achievements |
The aim of this study is to generate hair follicle structure using human induced pluripotent stem cell (iPSC)-derived epithelial and mesenchymal components optimized for trichogenicity. With regards to epithelial cells, a stable protocol to induce keratinocytes containing stem cell marker expressing cells was established. For trichogenic mesenchymal component preparation, an approach to generate multipotent mesenchymal stem cell-like cells was developed. The resultant cells are further programmed to acquire hair inductive property of dermal papilla cells. When co-grafted with normal human keratinocytes into immunedeficient mice, those cells gave rise to hair follicle-like structures with moderate reconstitution efficiency. However, regeneration of hair follicle structure exclusively consisted of human iPSC-derived cells is still technically very challenging, mainly because of relatively low efficiency and adjustment of the timing in the production of both iPSC-derived subsets.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] LNGFR+THY-1+ Human Pluripotent Stem Cell-derived Neural Crest-Like Cells Have the Potential to Develop Into Mesenchymal Stem Cells2016
Author(s)
Takehito Ouchi, Satoru Morikawa, Shinsuke Shibata, Kimiko Fukuda, Hironobu Okuno, Takumi Fujimura, Tatsuo Kuroda, Manabu Ohyama, Wado Akamatsu, Taneaki Nakagawa, Hideyuki Okano
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Journal Title
Differentiation
Volume: 印刷中
Related Report
Peer Reviewed / Acknowledgement Compliant
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