| Project/Area Number |
25293247
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
戸田 重誠 金沢大学, 大学病院, 講師 (00323006)
吉原 亨 京都大学, 医学(系)研究科(研究院), 助教 (00401935)
上田 なつ子 (辻野 / 辻野 なつ子 / 上田 なつ子(辻野)) 筑波大学, 国際統合睡眠医学研究機構, 助教 (40432166)
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| Research Collaborator |
Lewis David A University of Pittsburgh, Department of Psychiatry and Neuroscience
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Keywords | ヒト死後脳 / 霊長類モデル / 遺伝子改変マウス / real-time PCR / in situ hybridization / 脳神経疾患 / 死後脳 / モデルマウス / 死後脳研究 |
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| Outline of Final Research Achievements |
In the prefrontal cortex (PFC) of subjects with schizophrenia, alterations in parvalbumin (PV)-containing neurons are thought to contribute to cognitive deficits. Kv9.3 is a potassium channel subunit that is selectively expressed in PV neurons and critical to their roles in cognitive functions. Our cellular level quantification of the mRNA levels for KCNS3, the gene encoding Kv9.3, revealed that the number of KCNS3 mRNA-expressing neurons and the mRNA level per neuron were significantly reduced in the PFC of schizophrenia subjects. KCNS3 mRNA levels were unaffected in the PFC of monkeys exposed to antipsychotics, indicating that the lower KCNS3 expression is not solely due to the antipsychotic treatment. To test the effect of lower Kv9.3 subunit expression on cognitive functions, we inactivated the kcns3 gene in mice and confirmed reduced kcns3 mRNA expression in the PFC of these mice.
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