| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
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| Outline of Final Research Achievements |
We have developed rapid diagnostic technique for biological clock period and clock phase of the patients with circadian rhythm sleep disorders, and gathered clinical and pathophysiological research data to develop strategies for precise diagnosis and optimization of made-to-order chronotherapy. Skin fibroblasts from patients with delayed sleep phase type and non-entrained type, as well as healthy controls were obtained and transfected with the Bmal1-luc reporter construct Bp/527-LUC, and luminescence from the cells was continuously measured to detect the peripheral clock dysfunction of the disorders. As a results, non-entrained type patients showed significantly prolonged period of peripheral clock. Especially, patients with severely prolonged period suffered from rapidly dumped luminescence rhythm amplitude and poor response to chronotherapy, indicating that peripheral clock period properties could be useful surrogates to predict clinical outcomes of the disorder.
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