Elucidation of the action mechanism of combined treatment with X-ray irradiation and 5-aminolevulinic acid by trantcriptome analysis
| Project/Area Number |
25293270
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
Takahashi Junko 国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (80415702)
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| Co-Investigator(Kenkyū-buntansha) |
森 崇 岐阜大学, 応用生物科学部, 教授 (40402218)
岩橋 均 岐阜大学, 応用生物科学部, 教授 (60356540)
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| Project Period (FY) |
2013-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2017: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
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| Keywords | 放射線治療 / X線 / 放射線増感剤 / 5-アミノレブリン酸 / ポルフィリン / がん治療 / 放射線力学療法 / 光線力学療法 / 増感剤 / マイクロアレイ / アミノレブリン酸 / 放射線 / 放射線療法 |
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| Outline of Final Research Achievements |
Combined treatment with 5-aminolevulinic acid (ALA) and X-rays improves tumor suppression in vivo. This is because the accumulated protoporphyrin IX from ALA enhances the generation of ROS by the X-ray irradiation. In the present study, a high-energy medical linear accelerator was used instead of a non-medical low energy X-ray irradiator, which had been previously used. Tumor-bearing mice implanted with B16-BL6 melanoma cells were treated with fractionated doses of irradiation (in total, 20 or 30 Gy), using two types of X-ray irradiator after ALA administration. Suppression of tumor growth was enhanced with X-ray irradiation in combination with ALA treatment compared with X-ray treatment alone, using both medical and non-medical X-ray irradiators. “Radiodynamic therapy” using radiation from medical linacs as a physical driving force, rather than the light used in photodynamic therapy, may have potential clinical applications.
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Report
(5 results)
Research Products
(9 results)
