Increasing in size of 3-dimentional regenerative tissue by intra-structural perfusion system.
| Project/Area Number |
25293273
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KOYAMA Hiroyuki 東京大学, 医学部附属病院, 特任准教授 (10241994)
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| Co-Investigator(Kenkyū-buntansha) |
MIYATA Tetsuro 東京大学, 医学部附属病院, 登録診療員 (70190791)
HOSHINA Katsuyuki 東京大学, 医学部附属病院, 講師 (90571761)
保坂 晃弘 東京大学, 医学部附属病院, 助教 (90625170)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
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| Keywords | 血管新生 / 足場材料 / 三次元再生組織 / 大型化 / 生体材料 / ハイドロゲル / 人工臓器 / 再生医学 / 外科 / 細胞・組織 |
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| Outline of Final Research Achievements |
To construct a 3-dimensional regenerative tissue with vascular system, we selected an approach to accumulate cell-spheroids as building blocks and simultaneously infill angiogenesis-inducible matrix material among the spheroids for induction of neovascular in-growth. The angiogenesis-inducible matrix material has a function to rapidly induce neovascular growth from recipient bed into the inside of the material. The aim of the present study is to establish novel techniques which enable to increase in size of the regenerative tissue. For this purpose, we developed an intra-structural perfusion system of culture medium to preserve viability of cell-spheroids embedded in the material. Further, we also developed method for preparing vasculature-like structure in the material. The vasculature-like structure was constructed by culturing endothelial cells, and could rapidly become true vascular network after transplantation.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Long-Term Results of Treatment for Critical Limb Ischemia2015
Author(s)
Miyahara T, Suhara M, Nemoto Y, Shirasu T, Haga M, Mochizuki Y, Matsukura M, Akai T, Taniguchi R, Nemoto M, Yamamoto S, Nishiyama A, Hosaka A, Hoshina K, Okamoto H, Shigematsu K, Miyata T, Watanabe T.
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Journal Title
Annals of Vascular Diseases
Volume: 8
Issue: 3
Pages: 192-197
DOI
NAID
ISSN
1881-641X, 1881-6428
Related Report
Peer Reviewed
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[Journal Article] .Biomechanical analysis of an aortic aneurysm model and its clinical application to thoracic aortic aneurysms for defining "saccular" aneurysms.2015
Author(s)
Akai T, Hoshina K, Yamamoto S, Takeuchi H, Nemoto Y, Ohshima M, Shigematsu K, Miyata T, Yamauchi H, Ono M, Watanabe T.
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Journal Title
J Am Heart Assoc.
Volume: published online
Issue: 1
Pages: 1-11
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Bone regeneration by the combined use of tetrapod-shaped calcium phosphate granules with basic fibroblast growth factor-binding ion complex gel in canine segmental radial defects.2014
Author(s)
Honnami M, Choi S, Liu IL, Kamimura W, Taguchi T, Hojo H, Shimohata N, Ohba S, Koyama H, Nishimura R, Chung UI, Sasaki N, Mochizuki M.
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Journal Title
J Vet Med Sci.
Volume: 76
Pages: 955-961
NAID
Related Report
Peer Reviewed / Open Access
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