Analysis of the interaction between the folate receptor beta expression macrophages and cancer stem cells in pancreatic cancer invasion
Project/Area Number |
25293288
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Digestive surgery
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Research Institution | Kagoshima University |
Principal Investigator |
TAKAO SONSHIN 鹿児島大学, 医用ミニブタ・先端医療開発研究センター, 特任教授 (80171411)
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Co-Investigator(Kenkyū-buntansha) |
MATSUYAMA TAKAMI 鹿児島大学, 医用ミニブタ先端医療開発研究センター, 客員教授 (30145479)
Matsubara Shyichiro 鹿児島大学, 医用ミニブタ先端医療開発研究センター, 准教授 (60199841)
NAGAI TAKU 鹿児島大学, 医歯学域医学系, 講師 (90363647)
DING QIANG 聖ミカエル病院李嘉誠知識研究所, キーナン研究センター (80457647)
YOSHIMITSU MAKOTO 鹿児島大学, 医歯学域医学系, 准教授 (70404530)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2013: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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Keywords | pancreatic cancer / cancer stem cell / CD133 / macrophage / FRb-receptor / tumor microenvironment / miR-30 / FRbeta / microRNA / invasion / migration / FRb receptor / fibrotic stroma / nude mouse / EMT / N-cadherin / miRNA / caerulein誘導慢性膵炎 / FRβマクロファージ / CD68マクロファージ / ヒト化抗FRαβ抗体 |
Outline of Final Research Achievements |
In this study, we examine the relevance to the invasion of pancreatic fibrosis and cancer stem cells. CD133 expression pancreatic cancer cells showing the cancer stem cell-like characteristics showed fibrosis enhancement and high expression of active macrophages interstitial in the pancreas within the transplantation of immunocompromised mice. Many of the active macrophages were present in the invasion site express FRβ. To establish a blood soluble FRβ measurement system, a result of the study the correlation between the clinical data of pancreatic cancer 20 cases, serum soluble FRβ high-value group has a high possibility of lymph node metastasis, clinical application has been suggested. In addition, CD133 expression pancreatic cancer cells were significantly enhanced the hematogenous metastases through the expression of EMT-related genes. It was further found that the miR-30 family plays an important role in the invasion of CD133 expression pancreatic cancer cells.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Macrophage uptake and accumulation of folates are polarization-dependent in vitro and in vivo and are regulated by activin A.2014
Author(s)
Samaniego R, Palacios BS, Domiguez-Soto A, Vidal C, Salas A, Matsuyama T, Sánchez-Torres C, de la Torre I, Miranda-Carús ME, Sánchez-Mateos P, Puig-Kröger A.
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Journal Title
Journal of Leukocyte Biology
Volume: 95
Issue: 5
Pages: 797-808
DOI
Related Report
Peer Reviewed
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[Presentation] The roles of novel tumor-associated macrophages (TAMs) in invasion and metastasis of pancreatic cancer
Author(s)
Sonshin Takao, Qiang Ding, Taku Nagai, Hiroshi Kurahara, Makoto Yoshimitsu, Taisaku Kuwahata, Yumi Miyazaki, Ryoko Imakiire, Shoko Ueno, Toru Obara, Takami Matsuyama
Organizer
American Association for Cancer Research (AACR) Annual Meeting 2013
Place of Presentation
Washington DC, USA
Related Report
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