New surgical strategy for lung cancer based on circulating tumor cells

• Project/Area Number: 25293301
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Respiratory surgery
• Research Institution: Osaka University
• Principal Investigator: Sawabata Noriyoshi 大阪大学, 医学(系)研究科(研究院), 教授 (50403184)
• Co-Investigator(Kenkyū-buntansha): INOUE Masayoshi 京都府立医科大学, 医学系研究科, 教授 (10379232) ; OKUMURA Meinoshin 大阪大学, 医学系研究科, 教授 (40252647) ; FUNAKI SOuichirou 大阪大学, 医学系研究科, 講師 (50465241) ; SHINTANI Yasushi 大阪大学, 医学系研究科, 准教授 (90572983) ; 舟木 壮一郎 大阪大学, 医学(系)研究科(研究院), 助教 (50464251) ; 中桐 伴行 大阪大学, 医学(系)研究科(研究院), 研究員 (70528710)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2013: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
• Keywords: 肺癌 / 循環癌細胞 / 手術 / 再発 / 多施設共同研究 / 遊離癌細胞 / 外科的治療 / 上皮間葉移行 / 再発制御 / 外科治療 / 転移 / 微小環境

Outline of Final Research Achievements

Clinical implication of isolated tumor cells (ITC) in surgical cases of lung cancer were demonstrated using samples of extracted tumor cells at the surgical margin, circulating tumor cells in the blood drained pulmonary vein of the resected lung lobe and peripheral arterial blood. In detail, it is revealed that the morphology of ITC was associated with severity of stromal reaction which is a surrogate marker of epithelial-mesenchymal transition and the degree of tumor malignancy. In addition, cancer cell dislodgement by surgical manipulation is demonstrate and this phenomenon is confirmed in a multicenter prospective study.

Research Products

• [Journal Article] Lung excision of non-small-cell lung cancer leaves cancer cells in residual lobe: cytological detection using pulmonary vein blood. (2016)
  • Author(s): Sawabata N, Funaki S, Shintani Y, Okumura M.
  • Journal Title: Interact Cardiovasc Thorac Surg Volume: 22 Issue: 2 Pages: 131-5
  • DOI: 10.1093/icvts/ivv301
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Tumor Size, Margin Distance Rate, and Margin Cytologic Results Influence Recurrence and Survival After Wedge Resection for Lung Cancer. (2016)
  • Author(s): Sawabata N.
  • Journal Title: Ann Thorac Surg Volume: 101 Issue: 3 Pages: 1242-1242
  • DOI: 10.1016/j.athoracsur.2015.08.020
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Impact of cardiopulmonary complications of lung cancer surgery on long-term outcomes. (2015)
  • Author(s): Nojiri T, Inoue M, Takeuchi Y, Maeda H, Shintani Y, Sawabata N, Hamasaki T, Okumura M.
  • Journal Title: Surg Today Volume: 45 Issue: 6 Pages: 740-745
  • DOI: 10.1007/s00595-014-1032-z
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Prognosis of lung cancer patients in Japan according to data from the Japanese Joint Committee of Lung Cancer Registry. (2014)
  • Author(s): Sawabata N
  • Journal Title: Respir Investig Volume: 52 Issue: 6 Pages: 317-321
  • DOI: 10.1016/j.resinv.2014.04.002
  • Peer Reviewed / Open Access
• [Journal Article] Who should decide margin length in pulmonary resection of lung cancer? (2014)
  • Author(s): Sawabata N
  • Journal Title: J Thorac Cardiovasc Surg Volume: 148 Issue: 1 Pages: 370-371
  • DOI: 10.1016/j.jtcvs.2014.03.032
  • Peer Reviewed
• [Journal Article] Prognostic value of intraoperative pleural lavage cytology for non-small cell lung cancer: the influence of positive pleural lavage cytology results on T classification. (2014)
  • Author(s): Kameyama K, Okumura N, Miyaoka E, Asamura H, Yoshino I, Tada H, Fujii Y, Nakanishi Y, Eguchi K, Mori M, Kobayashi H, Sawabata N, Okumura M, Yokoi K
  • Journal Title: J Thorac Cardiovasc Surg Volume: 148 Issue: 6 Pages: 2659-2665
  • DOI: 10.1016/j.jtcvs.2014.07.090
  • Peer Reviewed
• [Journal Article] Clinicopathological characteristics and surgical results of lung cancer patients aged up to 50 years: the Japanese Lung Cancer Registry Study 2004. (2014)
  • Author(s): Inoue M, Okumura M, Sawabata N, Miyaoka E, Asamura H, Yoshino I, Tada H, Fujii Y, Nakanishi Y, Eguchi K, Mori M, Kobayashi H, Yokoi K.
  • Journal Title: Lung Cancer Volume: 82 Issue: 2 Pages: 246-251
  • DOI: 10.1016/j.lungcan.2013.11.007
  • Peer Reviewed
• [Journal Article] Evaluation of the new IASLC/ATS/ERS proposed classification of adenocarcinoma based on lepidic pattern in patients with pathological stage IA pulmonary adenocarcinoma. (2014)
  • Author(s): Nakagiri T, Sawabata N, Morii E, Inoue M, Shintani Y, Funaki S, Okumura M.
  • Journal Title: Gen Thorac Cardiovasc Surg. Volume: 62 Issue: 11 Pages: 671-7
  • DOI: 10.1007/s11748-014-0429-3
  • Peer Reviewed
• [Journal Article] Significance of tumour vessel invasion in determining the morphology of isolated tumour cells in the pulmonary vein in non-small-cell lung cancer (2013)
  • Author(s): Funaki S, Sawabata N, Abulaiti A, Nakagiri T, Shintani Y, Inoue M, Minami M, Okumura M
  • Journal Title: Eur J Cardiothorac Surg Volume: 43 Issue: 6 Pages: 1126-1130
  • DOI: 10.1093/ejcts/ezs553
  • Peer Reviewed
• [Journal Article] Locoregional recurrence after pulmonary sublobar resection of non-small cell lung cancer: can it be reduced by considering cancer cells at the surgical margin? (2013)
  • Author(s): Sawabata N
  • Journal Title: Gen Thorac Cardiovasc Surg Volume: 61 Issue: 1 Pages: 9-16
  • DOI: 10.1007/s11748-012-0156-6
  • Peer Reviewed
• [Journal Article] Pulmonary fibroblasts induce epithelial mesenchymal transition and some characteristics of stem cells in non-small cell lung cancer. (2013)
  • Author(s): Shintani Y, Abulaiti A, Kimura T, Funaki S, Nakagiri T, Inoue M, Sawabata N, Minami M, Morii E, Okumura M
  • Journal Title: Ann Thorac Surg Volume: 96 Pages: 425-433
  • Peer Reviewed
• [Journal Article] Is segmentectomy suitable for solid-type lung cancer? (2013)
  • Author(s): Sawabata N
  • Journal Title: J Thorac Cardiovasc Surg Volume: 146 Pages: 728-729
  • Peer Reviewed
• [Journal Article] Interaction between non-small-cell lung cancer cells and fibroblasts via enhancement of TGF-β signaling by IL-6. (2013)
  • Author(s): Abulaiti A, Shintani Y, Funaki S, Nakagiri T, Inoue M, Sawabata N, Minami M, Okumura M.
  • Journal Title: Lung Cancer Volume: 82 Issue: 2 Pages: 204-213
  • DOI: 10.1016/j.lungcan.2013.08.008
  • Peer Reviewed
• [Presentation] CirculatingTumorCell ; CTC の臨床的意義： (2015)
  • Author(s): 澤端章好 百武 威
  • Organizer: 第56回 日本肺癌学会学術集会
  • Place of Presentation: パシフィコ横浜 神奈川 横浜
  • Year and Date: 2015-11-26
• [Presentation] 積極的縮小肺切除における遊離癌細胞による再発ポテンシャルから見た術式選択 (2015)
  • Author(s): 澤端章好 舟木壮一郎、百武 威、吉川陽子、奥村名之進
  • Organizer: 第32回 日本呼吸器外科学会総会
  • Place of Presentation: サンポートホール高松 香川 高松
  • Year and Date: 2015-05-14
• [Presentation] 肺癌治療における自家肺移植：コミュニティーホスピタルでの実現の可能性 (2014)
  • Author(s): 澤端 章好
  • Organizer: 第31回日本呼吸器外科学会総会
  • Place of Presentation: ホテル日航東京（東京都港区）
  • Year and Date: 2014-05-10 – 2014-05-11
• [Presentation] In the new adenocarcinoma classification, the histopathological finding ”invasion”reflects the presence of isolated tumour cells (2013)
  • Author(s): Funaki S, Sawabata N, Okumura M
  • Organizer: 27th EACTSAnnual Meeting
  • Place of Presentation: Vienna, Austria
• [Presentation] 肺癌ニッチとしての部分切除後残存肺：摘出肺肺静脈血中 (2013)
  • Author(s): 澤端 章好、舟木 壮一郎、須崎 剛行、中桐 伴行、新谷 康、井上 匡美、南 正人、川村 知裕、野尻 崇、木村 亨、
  • Organizer: 第30回 日本呼吸器外科学会
  • Place of Presentation: 名古屋