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Use of combination of frozen tumor tissue and dendritic cell immunotherapy

Research Project

Project/Area Number 25293318
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Orthopaedic surgery
Research InstitutionKanazawa University

Principal Investigator

Nishida Hideji  金沢大学, 医学系, 協力研究員 (50569374)

Co-Investigator(Kenkyū-buntansha) Miwa Shinji  金沢大学, 大学病院, 医員 (40753455)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Keywords骨腫瘍 / 軟部腫瘍 / 樹状細胞 / 免疫療法 / 骨軟部肉腫 / TNFα / 骨軟部腫瘍に対する樹状細胞療法
Outline of Final Research Achievements

Thirty seven patients with metastatic or recurrent sarcomas were enrolled in this study. Peripheral blood mononuclear cells obtained from the patients were suspended in media containing IL-4 and GM-CSF. These cells were treated with tumor lysate, TNF-α, and OK-432. One treatment course comprised weekly DC injection for 6 times. Toxicity, clinical response (tumor volume, serum IFN-γ, and serum IL-12), and oncological outcomes were observed. DC therapy were performed in 37 patients. No severe adverse event and death associated with the DC injections were observed in the study patients. Increased serum IFN-γ and IL-12 were observed 1 month after DC injection. 6 cases had stable disease, and 1 case showed partial response 3 months after DC therapy. Of the 37 patients, 13 patients were alive and 1 patient was progression free at the time of the final follow-up. Overall survival rates and progression free survival rates of the patients at 3 years were 42.3% and 2.9%, respectively.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report

Research Products

(24 results)

All 2015 2014 2013

All Journal Article (13 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 12 results,  Open Access: 5 results,  Acknowledgement Compliant: 1 results) Presentation (11 results)

  • [Journal Article] Successful correction of tibial bone deformity through multiple surgical procedures, liquid nitrogen-pretreated bone tumor autograft, three-dimensional external fixation, and internal fixation in a patient with primary osteosarcoma: a case report.2015

    • Author(s)
      Takeuchi A, Yamamoto N, Shirai T, Nishida H, Hayashi K, Watanabe K, Miwa S, Tsuchiya H.
    • Journal Title

      BMC Surgery

      Volume: 15 Pages: 124-124

    • DOI

      10.1186/s12893-015-0112-3

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Frozen Autograft-Prosthesis Composite Reconstruction in Malignant Bone Tumors.2015

    • Author(s)
      Subhadrabandhu S, Takeuchi A, Yamamoto N, Shirai T, Nishida H, Hayashi K, Miwa S, Tsuchiya H.
    • Journal Title

      Orthopedics

      Volume: 38

    • DOI

      10.3928/01477447-20151002-59

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Salvage method for unplanned excision of soft tissue sarcoma: long-term results of second-look surgery following radio-hyperthermo-chemotherapy.2015

    • Author(s)
      Hayashi K, Yamada S, Inatani H, Okamoto H, Takeuchi A, Nishida H, Yamamoto N, Tsuchiya H, Otsuka T
    • Journal Title

      Anticancer Research

      Volume: 35 Pages: 493-8

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Heterogeneous cell-cycle behavior in response to UVB irradiation by a population of single cancer cells visualized by time-lapse FUCCI imaging.2015

    • Author(s)
      Miwa S, Yano S, Kimura H, Yamamoto M, Toneri M, Murakami T, Hayashi K, Yamamoto N, Fujiwara T, Tsuchiya H, Hoffman RM.
    • Journal Title

      Cell Cycle

      Volume: 14 Pages: 1932-1937

    • DOI

      10.1080/15384101.2015.1033598

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cell-cycle fate-monitoring distinguishes individual chemosensitive and chemoresistant cancer cells in drug-treated heterogeneous populations demonstrated by real-time FUCCI imaging.2015

    • Author(s)
      Miwa S, Yano S, Kimura H, Yamamoto M, Toneri M, Matsumoto Y, Uehara F, Hiroshima Y, Murakami T, Hayashi K, Yamamoto N, Bouvet M, Fujiwara T, Tsuchiya H, Hoffman RM.
    • Journal Title

      Cell Cycle

      Volume: 14 Pages: 621-629

    • DOI

      10.4161/15384101.2014.991604

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Salvage method for unplanned excision of soft tissue sarcoma: long-term results of second-look surgery following radio-hyperthermo-chemotherapy.2015

    • Author(s)
      Hayashi K, Yamada S, Inatani H, Okamoto H, Takeuchi A, Nishida H, Yamamoto N, Tsuchiya H, Otsuka T.
    • Journal Title

      Anticancer Res

      Volume: 35 Pages: 493-8

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficacy of triplet regimen antiemetic therapy for chemotherapy-induced nausea and vomiting (CINV) in bone and soft tissue sarcoma patients receiving highly emetogenic chemotherapy, and an efficacy comparison of single-shot palonosetron and consecutive-day granisetron for CINV in a randomized, single-blinded crossover study.2015

    • Author(s)
      Kimura H, Yamamoto N, Shirai T, Nishida H, Hayashi K, Tanzawa Y, Takeuchi A, Igarashi K, Inatani H, Shimozaki S, Kato T, Aoki Y, Higuchi T, Tsuchiya H.
    • Journal Title

      Cancer Med

      Volume: 4 Pages: 333-341

    • DOI

      10.1002/cam4.373

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Late recurrence of osteosarcoma: a report of two cases.2014

    • Author(s)
      Igarashi K, Yamamoto N, Shirai T, Nishida H, Hayashi K, Tanzawa Y, Kimura H, Takeuchi A, Miwa S, Inatani H, Shimozaki S, Kato T, Tsuchiya H.
    • Journal Title

      J Orthop Surg (Hong Kong)

      Volume: 22 Pages: 415-9

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Outcomes and complications of reconstruction using tumor-bearing frozen autografts in patients with metastatic bone tumors.2014

    • Author(s)
      Miwa S, Takeuchi A, Shirai T, Yamamoto N, Hayashi K, Nishida H, Kimura H, Igarashi K, Tsuchiya H.
    • Journal Title

      Anticancer Res

      Volume: 34 Pages: 5569-77

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synovial sarcoma in knee joint, mimicking low-grade sarcoma confirmed by molecular detection of SYT gene split.2014

    • Author(s)
      Kimura H, Yamamoto N, Nishida H, Hayashi K, Takeuchi A, Nojima T, Ikeda H, Sawada-Kitamura S, Tsuchiya H.
    • Journal Title

      Anticancer Res

      Volume: 34 Pages: 3105-11

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Antimicrobial megaprostheses supported with iodine.2014

    • Author(s)
      Shirai T, Tsuchiya H, Nishida H, Yamamoto N, Watanabe K, Nakase J, Terauchi R, Arai Y, Fujiwara H, Kubo T.
    • Journal Title

      J Biomater Appl

      Volume: 29 Pages: 617-23

    • DOI

      10.1177/0885328214539365

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pedicle versus free frozen autograft for reconstruction in malignant bone and soft tissue tumors of the lower extremities2014

    • Author(s)
      Shimozaki S, Yamamoto N, Shirai T, Nishida H, Hayashi K, Tanzawa Y, Kimura H, Takeuchi A, Igarashi K, Inatani H, Kato T, Tsuchiya H
    • Journal Title

      J Orthop Sci.

      Volume: 19(1) Pages: 156-163

    • DOI

      10.1007/s00776-013-0487-x

    • NAID

      120005349170

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diagnosis and treatment of low-grade osteosarcoma : experience with nine cases2014

    • Author(s)
      Hayashi K, Tsuchiya H, Yamamoto N, Shirai T, Nishida H, Takeuchi A, Kimura H, Miwa S, Inatani H, Okamoto H, Yamada S, Ikeda H, Sawada-Kitamura S, Nojima T, Ooi A, Otsuka T
    • Journal Title

      Int J Clin Oncol

      Volume: Jul 24 Pages: 731-8

    • DOI

      10.1007/s10147-013-0592-z

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] Clinical outcomes and complications of reconstruction using tumor-bearing frozen autografts in patients with metastatic bone tumors2015

    • Author(s)
      Shinji Miwa, Norio Yamamoto, Katsuhiro Hayashi, Akihiko Takeuchi, Hiroyuki Inatani, Yu Aoki, Takashi Higuchi, Kensaku Abe, Hiroyuki Tsuchiya
    • Organizer
      第42回日本低温医学会
    • Place of Presentation
      金沢
    • Year and Date
      2015-11-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 骨肉腫の術前化学療法後の画像変化と予後の相関についての検討2015

    • Author(s)
      三輪真嗣,山本憲男,林克洋,武内章彦,稲谷弘幸,青木裕,樋口貴史,阿部健作,土屋弘行
    • Organizer
      第48 回 日本整形外科学会骨・軟部腫瘍学術集会
    • Place of Presentation
      香川
    • Year and Date
      2015-07-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] 骨肉腫マウスモデルに対する蛍光ガイド下手術の検討2015

    • Author(s)
      三輪真嗣,上原史成,木村浩明,林克洋,山本憲男,Robert M. Hoffman,土屋弘行
    • Organizer
      第88回日本整形外科学会学術総会
    • Place of Presentation
      神戸
    • Year and Date
      2015-05-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] 悪性骨軟部腫瘍に対する免疫療法(樹状細胞療法)の可能性2014

    • Author(s)
      西田英司、山本憲男、林克洋、武内章彦、下崎真吾、加藤貴士、樋口貴史、青木裕、土屋 弘行
    • Organizer
      第29回日本整形外科学会基礎学術集会
    • Place of Presentation
      城山観光ホテル(鹿児島県鹿児島市)
    • Year and Date
      2014-11-09 – 2014-11-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] 悪性骨軟部腫瘍に対する腫瘍破砕物質を抗原としTNF-αを併用した樹状細胞を用いた免疫療法2014

    • Author(s)
      西田英司、山本憲男、武内章彦、木村浩明、野村一世、土屋弘行
    • Organizer
      第52回日本癌治療学術集会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-08-28 – 2014-08-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] 標準治療抵抗性の悪性骨・軟部腫瘍に対する樹状細胞療法の可能性2014

    • Author(s)
      西田英司、山本憲男、白井寿治、林克洋、武内章彦、丹沢義一、木村浩明、三輪真嗣、五十嵐健太郎、下崎真吾、加藤貴士、青木裕、樋口貴史、土屋弘行
    • Organizer
      第47回日本整形外科学会 骨・軟部腫瘍学術集会
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Year and Date
      2014-07-17 – 2014-07-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Immunotherapy based on dendritic cells is feasible for patients with malignant bone and soft tissue tumours2014

    • Author(s)
      Hideji Nishida, Norio Yamamoto, Toshiharu Shirai, Katsuhiro Hayashi, Akihiko Takeuchi, Yoshikazu Tanzawa, Hiroaki Kimura, Shinji Miwa, Kentaro Igarashi, Hiroyuki Tsuchiya
    • Organizer
      2014 American Society of Clinical Oncology
    • Place of Presentation
      MaCormick Place (Chicago, Ilinois)
    • Year and Date
      2014-05-30 – 2014-06-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] Dendritic cells immunotherapy for patients with malignant bone and soft tissue tumours2014

    • Author(s)
      Hideji Nishida, Norio Yamamoto, Toshiharu Shirai, Katsuhiro Hayashi, Akihiko Takeuchi, Yoshikazu Tanzawa, Hiroaki Kimura, Shinji Miwa, Kentaro Igarashi, Hiroyuki Tsuchiya
    • Organizer
      第86回 日本整形外科学会総会
    • Place of Presentation
      神戸ポートピアホテル(兵庫県神戸市)
    • Year and Date
      2014-05-22 – 2014-05-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] 悪性骨軟部腫瘍に対する腫瘍破砕物質を抗原としTNF-αを併用した樹状細胞を用いた免疫療法2013

    • Author(s)
      西田英司、山本憲男、武内章彦、木村浩明、野村一世、土屋弘行
    • Organizer
      第120回中部日本整形外科災害外科学会
    • Place of Presentation
      和歌山 和歌山県民文化会館・ホテルアバローム紀の国
    • Related Report
      2013 Annual Research Report
  • [Presentation] Immunotherapy based on dendritic cells is feasible for patients with malignant bone and soft tissue tumours2013

    • Author(s)
      Hideji Nishida, Norio Yamamoto, Akihiko Takeuchi, Yoshikazu Tanzawa, Hiroaki Kimura, Munetomo Takata, Isei Nomura, Shinji Miwa, Takashi Kato, Hiroyuki Tsuchiya
    • Organizer
      26th European Musculoskeletal Oncology Society Meeting
    • Place of Presentation
      ヨーテボリ、スウェーデンSwedish Exhibition and Congress Center
    • Related Report
      2013 Annual Research Report
  • [Presentation] Immunotherapy based on dendritic cells is feasible for patients with malignant bone and soft tissue tumours2013

    • Author(s)
      Hideji Nishida, Norio Yamamoto, Toshiharu Shirai, Katsuhiro Hayashi, Akihiko Takeuchi, Yoshikazu Tanzawa, Hiroaki Kimura, Shinji Miwa, Kentaro Igarashi, Hiroyuki Tsuchiya
    • Organizer
      International Society of Limb Salvage 17th General Meeting
    • Place of Presentation
      ボローニャ、イタリア
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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