|Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
|Outline of Final Research Achievements
We analyzed that (1) the expression pattern of two isoforms (co-operative p53 β and competitive Δ 133p53) of p53, a major regulator for cellular senescence, in senescence of human mesenchymal stem cells (MSCs) and their ability of differentiation into chondrocytes, and (2) whether overexpression of p53 β in young MSCs induces senescence or overexpression of Δ 133p53 in old (pre-senescent) MSCs inhibits senescence and extends cellular lifespan, and molecular mechanism of their differentiation into chondrocytes. Expression of Δ 133p53, similar results with our previous reports in normal human fibroblasts and normal human CD8 positive T lymphocytes, extended MSC’s lifespan and up regulated the ability of differentiation into chondrocytes. We also found the unique molecular mechanism of Δ 133p53 by metabolome analysis and RNA-sequence, and have studied the detail mechanism.