Elucidating mechanisms of non-cell-autonomous osteoblast activation

• Project/Area Number: 25293327
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Keio University
• Principal Investigator: MATSUO Koichi 慶應義塾大学, 医学部, 教授 (40229422)
• Co-Investigator(Renkei-kenkyūsha): TAKADA Ichiro 慶應義塾大学, 医学部, 特任講師 (50361655)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2015: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: 骨芽細胞 / 骨形成 / 毛細血管 / 骨形成性血管 / 転写因子 / トランスジェニックマウス / 骨代謝 / 遺伝子

Outline of Final Research Achievements

Transgenic mice overexpressing the transcription factor Fra-1 (Fra-1 mice) exhibit activation of bone-forming osteoblasts leading to enhanced bone formation. We therefore analyzed mechanisms by which bone formation is enhanced in Fra-1 mice. We found that "osteogenic capillaries" form bone matrix around the capillaries in the auditory ossicle malleus during endochondral ossification. We propose that Fra-1 overexpression enhances systemic bone formation by employing osteogenic capillaries in a cell-non-autonomous manner.

Research Products

• [Int'l Joint Research] 国立がん研究センター/-/-(スペイン)
• [Journal Article] Osteocyte-directed bone demineralization along canaliculi. (2016)
  • Author(s): Nango N., Kubota S., Hasegawa T., Yashiro W., Momose A., Matsuo K.
  • Journal Title: Bone. Volume: 84 Pages: 279-288
  • DOI: 10.1016/j.bone.2015.12.006
  • Peer Reviewed / Open Access
• [Journal Article] Osteogenic capillaries orchestrate growth plate-independent ossification of the malleus (2015)
  • Author(s): Matsuo K, Kuroda Y, Nango N, Shimoda K, Kubota Y, Ema M, Bakiri L, Wagner EF, Takeda Y, Yashiro W, Momose A
  • Journal Title: Development Volume: 142 Pages: 3912-20
  • DOI: 10.1242/dev.123885
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Talbot-defocus multiscan tomography using the synchrotron X-ray microscope to study the lacuno-canalicular network in mouse bone (2013)
  • Author(s): Nango, N., Kubota, S., Takeuchi, A., Suzuki, Y., Yashiro, W., Momose, A. and Matsuo, K.
  • Journal Title: Opt. Express Volume: ４ Issue: 6 Pages: 917-923
  • DOI: 10.1364/boe.4.000917
  • Peer Reviewed
• [Presentation] 骨形成性毛細血管による耳小骨や長管骨の石灰化 (2016)
  • Author(s): 松尾光一
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: ビッグパレットふくしま（福島県郡山市）
  • Year and Date: 2016-03-28
  • Invited
• [Presentation] Continuous Parathyroid Hormone Injection in Mouse Has Differential Effects on Osteoclast Activation in Primary and Secondary Spongiosa. (2015)
  • Author(s): Nango Nobuhito, Kubota Shogo, Yashiro Wataru, Momose Atsushi, Ichinose Shizuko, Matsuo Koichi.
  • Organizer: Annual Meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: シアトル（米国）
  • Year and Date: 2015-10-09
  • Int'l Joint Research
• [Presentation] Sialylated Glycans of MMP-9 Mark Bone Resorption Lacunae. (2015)
  • Author(s): Kuroda Yukiko, Kuno Atsushi, Narimatsu Hisashi, Matsuo Koichi.
  • Organizer: Annual Meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: シアトル（米国）
  • Year and Date: 2015-10-09
  • Int'l Joint Research
• [Presentation] Osteogenic Capillaries Spatially Orient Pericapillary Osteoblasts to Direct Endochondral Ossification of the Malleus (2014)
  • Author(s): Takada Ichiro, Shimoda Kouji, Kubota Yoshiaki, Ema Masatsugu, Takeda Yoshihiro, Nobuhito Nango, Yashiro Wataru, Momose Atsushi, Bakiri Latifa, Wagner Erwin, Matsuo Koichi
  • Organizer: Annual Meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: Houston, Texas, USA
  • Year and Date: 2014-09-12 – 2014-09-15
• [Presentation] 骨における細胞間相互作用の解明に向けて（学術賞受賞講演） (2014)
  • Author(s): 松尾光一
  • Organizer: 第32回日本骨代謝学会学術集会
  • Place of Presentation: 大坂
  • Year and Date: 2014-07-24 – 2014-07-26
  • Invited
• [Presentation] Pericapillary Bone Formation by Mural Osteoblasts during Endochondral Ossification (2013)
  • Author(s): Ichiro Takada, Nobuhito Nango, Yoshihiro Takeda, Atsushi Momose, Yoshiaki Kubota, Sho Kanzaki, Kouji Shimoda, Yasunari Takada, Latifa Bakiri, Erwin Wagner and Koichi Matsuo.
  • Organizer: 35th Annual Meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: Baltimore, Maryland, USA
• [Remarks] 慶應義塾大学医学部 松尾光一（細胞組織学）研究室
  • URL: http://wp4.matsuo-lab.com/
• [Remarks] 慶應義塾大学医学部 松尾光一（細胞組織学）研究室
  • URL: http://www.matsuo-lab.com/