| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Outline of Final Research Achievements |
GJB2 encodes connexin (Cx) 26, a component in cochlear gap junction. We recently demonstrated that the drastic disruption of gap junction plaque (GJP) macromolecular complex composed of Cx26 and Cx30 are critical pathogenesis starting before hearing onset. To develop the effective therapy for GJB2 associated hearing loss, restoration of gap junction plaque (GJP) macromolecular complex using virus vectors or multipotent stem cells such as induced pluripotent stem (iPS) cells and mescenchymal stem cell (MSC) are expected to rescue the hearing function of GJB2 related hearing loss. Mouse induced pluripotent stem cells (iPS) were used for generation of Cx26-expressing cells with proper gap junction plaque between the cells. Adeno associate virus (AAV) were used for the GJB2 gene transfer and restoration of GJP. By differentiation of iPS cells, we generated the Cx26-expressiong cells with large gap junction plaque as cochlear cells.
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