| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Outline of Final Research Achievements |
We demonstrated experimentally that immune responses can be avoided when retinal pigment epithelial (RPE) cells generated from iPSCs derived from a MHC/HLA homozygous donor are matched with MHC/HLA of recipient. Our research team developed an in vivo experimental method in a primate model, in which they transplanted RPE cells derived from iPSCs from a MHC homozygote monkey into a different monkey (MHC-matched), and an in vitro experimental system using RPE cells derived from human iPSCs and co-cultured them with human immune cells. In vivo data, no signs of immune rejection were observed in recipients of MHC-matched grafts, and the sheet grafts survived for at least six months post-transplantation. Similarly, the matching of at least three HLA gene loci (HLA-A, HLA-B, and HLA-DRBI) between iPSCs-derived RPE cells and the donor immune cells was sufficient for preventing the trigger of an immune response in vitro. Our study was published in two separate papers in Stem Cell Reports.
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