| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2013: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Outline of Final Research Achievements |
About 200 Tissue samples of the cases treated by JPLT-2 protocol and several hepatoblastoma cell lines were used. SNP array analysis revealed the high frequency of gain and loss at chromosomes 1p. 1q and 2q. Whole genome expression analysis using microarray detected 12 significantly upregulated genes located the aberrant chromosomes in poor prognostic cases. Among them, fluorescent real-time PCR confirmed 3 genes significantly upregulated in these unfavorable cases and these were involved in Wnt and MAPK signals and correlated with activation of mucin and AFP production. These genes inserted into hepatoblastoma cell lines and 2 of them showed upregulated cell growth, suggesting that these genes might be molecular targets of hepatoblastoma.
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